Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SHEW_RS05870 Genome accession   NC_009092
Coordinates   1313320..1314432 (+) Length   370 a.a.
NCBI ID   WP_011864943.1    Uniprot ID   A3QC12
Organism   Shewanella loihica PV-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1308320..1319432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEW_RS05835 (Shew_1132) rdgB 1308471..1309070 (-) 600 WP_041407003.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHEW_RS05840 (Shew_1133) - 1309212..1309646 (-) 435 WP_011864937.1 DUF4426 domain-containing protein -
  SHEW_RS05845 (Shew_1134) yggU 1309733..1310023 (-) 291 WP_011864938.1 DUF167 family protein YggU -
  SHEW_RS05850 (Shew_1135) - 1310024..1310572 (-) 549 WP_011864939.1 YggT family protein -
  SHEW_RS05855 (Shew_1136) proC 1310633..1311451 (-) 819 WP_011864940.1 pyrroline-5-carboxylate reductase -
  SHEW_RS05860 (Shew_1137) - 1311533..1312234 (-) 702 WP_011864941.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEW_RS05865 (Shew_1138) pilT 1312270..1313307 (+) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEW_RS05870 (Shew_1139) pilU 1313320..1314432 (+) 1113 WP_011864943.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEW_RS05875 (Shew_1140) hemH 1314613..1315626 (+) 1014 WP_041406501.1 ferrochelatase -
  SHEW_RS05880 (Shew_1141) - 1316043..1317056 (-) 1014 WP_011864733.1 IS110-like element ISSlo1 family transposase -
  SHEW_RS05885 (Shew_1142) - 1317630..1319018 (+) 1389 WP_011864945.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41143.26 Da        Isoelectric Point: 6.3733

>NTDB_id=27710 SHEW_RS05870 WP_011864943.1 1313320..1314432(+) (pilU) [Shewanella loihica PV-4]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKGLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEVLINTPRVASIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=27710 SHEW_RS05870 WP_011864943.1 1313320..1314432(+) (pilU) [Shewanella loihica PV-4]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTGGAGCGCAAGGCGTCGGATCTCTTCATCACCGCCGGCTTTCCCCCCAG
CGCTAAGGTTGATGGCGAGGTGCGCCCCTTAGCCGAGAACCCTTTTACCCCGGCCCAGTCGCTGGAGTTTGTCGAGGCCC
TGATGACAGAGGCCCAGCGCAAAGAGTTTCATGAGACCCGCGAATGTAACTTTGCCTATGGCGAGAAGGGCCTGGGACGT
TTTCGTGTCAGCGCCTTCTGGCAGCGCGAGTCACCAGGTTGCGTGATGCGTCGCATCGAGACCAAGATCCCGCTGGTGGA
AGATCTTAAGCTGCCACCGATCCTGAAAGACCTAGTGATGAGTAAGCGTGGCCTTATCATCATGGTGGGTGGTACGGGTA
CAGGTAAGTCGACCTCGCTGGCGGCGCTTGTGGGTTACCGCAACTCCCACGCCCGTGGTCATATCCTCACCATCGAAGAT
CCGGTGGAATTTGTCCACGACCATCGCAAGAGCATCATCACCCAGCGTGAGGTGGGCATAGATACCGACTCGTTCGATGC
CGCGCTCAAGAGTTCACTGCGTCAGGCGCCGGATGTGATCCTGATCGGTGAGATCCGTACCCAGGAGACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGTCACCTCTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCCGAGAGTAAGCACCAGCAGCTGCTGTTCGACTTGTCGCTGAACCTTCGCGGCATCGTAGCGCAGCA
ACTTGTGCCTAAGGCAGATGGCACGGGTCGCCGGGCGGCCATCGAGGTGCTGATCAACACCCCAAGGGTTGCCAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCCAAATCCAACGAGCAGGGGATGCAGACCTTCGACCAAGCG
CTGCTCAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCGCTGCACCACGCCGACTCGCCAAACGATCTGCGTCT
GATGATCAAGCTGCAAAGCTCAGAATCGGCAAGCTCAGGCTTCATGGAAGGTGTGACGCTGGATCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3QC12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Acinetobacter baylyi ADP1

59.889

97.027

0.581
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment