Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1N78_RS20890 Genome accession   NZ_CP027300
Coordinates   4354145..4355089 (-) Length   314 a.a.
NCBI ID   WP_110147409.1    Uniprot ID   -
Organism   Serratia marcescens strain SGAir0764     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4349145..4360089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1N78_RS20860 (C1N78_20830) - 4349945..4350457 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  C1N78_RS20865 (C1N78_20835) endA 4350559..4351254 (+) 696 WP_049300546.1 deoxyribonuclease I -
  C1N78_RS20870 (C1N78_20840) rsmE 4351324..4352055 (+) 732 WP_049300547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1N78_RS20875 (C1N78_20845) gshB 4352066..4353016 (+) 951 WP_016930084.1 glutathione synthase -
  C1N78_RS20880 (C1N78_20850) - 4353163..4353726 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  C1N78_RS20885 (C1N78_20855) ruvX 4353726..4354148 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  C1N78_RS20890 (C1N78_20860) pilT 4354145..4355089 (-) 945 WP_110147409.1 type IV pilus twitching motility protein PilT Machinery gene
  C1N78_RS20895 (C1N78_20865) - 4355110..4355817 (+) 708 WP_033639392.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1N78_RS20900 (C1N78_20870) proC 4355837..4356658 (+) 822 WP_110147410.1 pyrroline-5-carboxylate reductase -
  C1N78_RS20905 (C1N78_20875) - 4356690..4357244 (+) 555 WP_110147411.1 YggT family protein -
  C1N78_RS20910 (C1N78_20880) yggU 4357241..4357534 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  C1N78_RS20915 (C1N78_20885) - 4357675..4358268 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  C1N78_RS20920 (C1N78_20890) hemW 4358261..4359403 (+) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  C1N78_RS20925 (C1N78_20895) - 4359442..4359876 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33650.52 Da        Isoelectric Point: 7.5744

>NTDB_id=276218 C1N78_RS20890 WP_110147409.1 4354145..4355089(-) (pilT) [Serratia marcescens strain SGAir0764]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSTGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVIA
QKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQGLQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 945 bp        

>NTDB_id=276218 C1N78_RS20890 WP_110147409.1 4354145..4355089(-) (pilT) [Serratia marcescens strain SGAir0764]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCACGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGACACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGATCGCG
CAAAAGCTCATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCT
GATCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAG
GTTTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

100

0.541
  pilT Vibrio cholerae strain A1552

51.515

100

0.541
  pilT Acinetobacter baylyi ADP1

47.13

100

0.497
  pilT Neisseria meningitidis 8013

45.882

100

0.497
  pilT Acinetobacter baumannii strain A118

46.364

100

0.487
  pilT Acinetobacter nosocomialis M2

46.364

100

0.487
  pilT Acinetobacter baumannii D1279779

46.364

100

0.487
  pilT Pseudomonas aeruginosa PAK

44.868

100

0.487
  pilT Neisseria gonorrhoeae MS11

45.266

100

0.487
  pilT Legionella pneumophila strain ERS1305867

44.412

100

0.481
  pilT Legionella pneumophila strain Lp02

44.412

100

0.481
  pilT Pseudomonas stutzeri DSM 10701

44.248

100

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.625

100

0.455
  pilU Vibrio cholerae strain A1552

38.037

100

0.395
  pilU Acinetobacter baylyi ADP1

38.39

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

36.283

100

0.392
  pilB Haemophilus influenzae 86-028NP

30.894

100

0.363

Multiple sequence alignment