Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SBAL_RS15555 Genome accession   NC_009052
Coordinates   3548696..3549733 (-) Length   345 a.a.
NCBI ID   WP_011847366.1    Uniprot ID   A3D6Z3
Organism   Shewanella baltica OS155     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3543696..3554733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL_RS15520 (Sbal_3017) - 3543802..3544119 (+) 318 WP_006082510.1 hypothetical protein -
  SBAL_RS15525 (Sbal_3018) yciH 3544298..3544627 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  SBAL_RS15530 (Sbal_3019) - 3544694..3545257 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  SBAL_RS15535 (Sbal_3020) ruvX 3545287..3545712 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SBAL_RS15540 (Sbal_3021) hemH 3545801..3546814 (-) 1014 WP_006085703.1 ferrochelatase -
  SBAL_RS15545 (Sbal_3022) - 3546847..3547386 (-) 540 WP_041408966.1 glutathione peroxidase -
  SBAL_RS15550 (Sbal_3023) pilU 3547574..3548686 (-) 1113 WP_011847365.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBAL_RS15555 (Sbal_3024) pilT 3548696..3549733 (-) 1038 WP_011847366.1 type IV pilus twitching motility protein PilT Machinery gene
  SBAL_RS15560 (Sbal_3025) - 3549769..3550467 (+) 699 WP_011847367.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBAL_RS15565 (Sbal_3026) proC 3550683..3551501 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SBAL_RS15570 (Sbal_3027) - 3551550..3552098 (+) 549 WP_011847368.1 YggT family protein -
  SBAL_RS15575 (Sbal_3028) yggU 3552098..3552397 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  SBAL_RS15580 (Sbal_3029) - 3552529..3552963 (+) 435 WP_011847369.1 DUF4426 domain-containing protein -
  SBAL_RS15585 (Sbal_3030) rdgB 3553103..3553720 (+) 618 WP_011847370.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38327.97 Da        Isoelectric Point: 6.9095

>NTDB_id=27596 SBAL_RS15555 WP_011847366.1 3548696..3549733(-) (pilT) [Shewanella baltica OS155]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTSMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=27596 SBAL_RS15555 WP_011847366.1 3548696..3549733(-) (pilT) [Shewanella baltica OS155]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACCTGCCCGCGCTCGATCACCAAGGCGTACACAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCCGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTGGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGGCCAACGGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTACACCAAAATAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGATCCGGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCTGCAGAAACGGGCCACTTAGTGTTTGGTACCTTGCATACCACCTCCGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCGGAATCACTGCAAGCGGTTATTTCACAAAC
CCTGATTAAAAAGGTAGGCGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACTCCCGCTATTCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCAGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3D6Z3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter baylyi ADP1

77.551

99.42

0.771
  pilT Pseudomonas aeruginosa PAK

78.402

97.971

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.193

99.13

0.765
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.263

98.261

0.484
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment