Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBAL_RS15550 Genome accession   NC_009052
Coordinates   3547574..3548686 (-) Length   370 a.a.
NCBI ID   WP_011847365.1    Uniprot ID   A3D6Z2
Organism   Shewanella baltica OS155     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3542574..3553686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL_RS15515 (Sbal_3016) - 3543063..3543623 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  SBAL_RS15520 (Sbal_3017) - 3543802..3544119 (+) 318 WP_006082510.1 hypothetical protein -
  SBAL_RS15525 (Sbal_3018) yciH 3544298..3544627 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  SBAL_RS15530 (Sbal_3019) - 3544694..3545257 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  SBAL_RS15535 (Sbal_3020) ruvX 3545287..3545712 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SBAL_RS15540 (Sbal_3021) hemH 3545801..3546814 (-) 1014 WP_006085703.1 ferrochelatase -
  SBAL_RS15545 (Sbal_3022) - 3546847..3547386 (-) 540 WP_041408966.1 glutathione peroxidase -
  SBAL_RS15550 (Sbal_3023) pilU 3547574..3548686 (-) 1113 WP_011847365.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBAL_RS15555 (Sbal_3024) pilT 3548696..3549733 (-) 1038 WP_011847366.1 type IV pilus twitching motility protein PilT Machinery gene
  SBAL_RS15560 (Sbal_3025) - 3549769..3550467 (+) 699 WP_011847367.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBAL_RS15565 (Sbal_3026) proC 3550683..3551501 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SBAL_RS15570 (Sbal_3027) - 3551550..3552098 (+) 549 WP_011847368.1 YggT family protein -
  SBAL_RS15575 (Sbal_3028) yggU 3552098..3552397 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  SBAL_RS15580 (Sbal_3029) - 3552529..3552963 (+) 435 WP_011847369.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41004.16 Da        Isoelectric Point: 6.2352

>NTDB_id=27595 SBAL_RS15550 WP_011847365.1 3547574..3548686(-) (pilU) [Shewanella baltica OS155]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSMDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=27595 SBAL_RS15550 WP_011847365.1 3547574..3548686(-) (pilU) [Shewanella baltica OS155]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTCATCACAGCGGGCTTTCCGCCAAG
TGCCAAGATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCAATGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCAACTCGTGAGTGTAACTTTGCTTTTGCTGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTTTGGCAGCGTGAATCGCCAGGCTGCGTGATGCGTCGTATCGAAACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCACCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATTATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCAACATCTCTGGCGGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCAGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACTCAGCGTGAAGTGGGCATAGATACTGAGTCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAACAGTTATTGTTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGTCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAGTTGCAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3D6Z2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment