Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FORC77_RS02440 Genome accession   NZ_CP027030
Coordinates   500282..501388 (+) Length   368 a.a.
NCBI ID   WP_011079463.1    Uniprot ID   A0A3Q0L3V4
Organism   Vibrio vulnificus strain FORC_077     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495282..506388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS02405 (FORC77_0425) - 495662..496264 (-) 603 WP_011079456.1 XTP/dITP diphosphatase -
  FORC77_RS02410 (FORC77_0426) - 496275..496706 (-) 432 WP_011079457.1 DUF4426 domain-containing protein -
  FORC77_RS02415 (FORC77_0427) yggU 496749..497039 (-) 291 WP_011151199.1 DUF167 family protein YggU -
  FORC77_RS02420 (FORC77_0428) - 497039..497596 (-) 558 WP_013571095.1 YggT family protein -
  FORC77_RS02425 (FORC77_0429) proC 497638..498456 (-) 819 WP_045618603.1 pyrroline-5-carboxylate reductase -
  FORC77_RS02430 (FORC77_0430) - 498491..499201 (-) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC77_RS02435 (FORC77_0431) pilT 499226..500263 (+) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC77_RS02440 (FORC77_0432) pilU 500282..501388 (+) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC77_RS02445 (FORC77_0433) ruvX 501481..501903 (-) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  FORC77_RS02450 (FORC77_0434) - 501925..502488 (-) 564 WP_011079465.1 YqgE/AlgH family protein -
  FORC77_RS02455 (FORC77_0435) gshB 502543..503496 (-) 954 WP_013571098.1 glutathione synthase -
  FORC77_RS02460 (FORC77_0436) rsmE 503511..504242 (-) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FORC77_RS02465 (FORC77_0437) - 504480..505814 (+) 1335 WP_045618605.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41245.35 Da        Isoelectric Point: 6.7296

>NTDB_id=274583 FORC77_RS02440 WP_011079463.1 500282..501388(+) (pilU) [Vibrio vulnificus strain FORC_077]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEERRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=274583 FORC77_RS02440 WP_011079463.1 500282..501388(+) (pilU) [Vibrio vulnificus strain FORC_077]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGCGAGCTGCGCCCGCAAGGCGAAAAATTATCGCAAATTGAAGTCGCTCAACTGCTGGATGGGGCGA
TGGACGAAGAGCGGCGCGCAGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGACGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTACCTGGCGCGGTGATTCGCCGCATTGAAACCAACATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAACGTGGATTGGTGCTGGTGGTGGGCGCGACGGGCTCTGGTA
AATCGACCACCATGGCTGCGATGACAGGATATCGAAATACCCACCGTAGTGGACACATTTTGACGGTGGAAGATCCGATT
GAATTTGTCCACGAACATAAACGCTGTATTGTCACCCAGCGTGAAGTCGGTTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTAATTGGTGAGATTCGCAGTCGGGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCAATGAACTTAAAAGGGGTAGTTGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTAGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCCGATTCGGCCAATGATTTACGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361

Multiple sequence alignment