Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FORC77_RS02435 Genome accession   NZ_CP027030
Coordinates   499226..500263 (+) Length   345 a.a.
NCBI ID   WP_026050359.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_077     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494226..505263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS02400 (FORC77_0424) hemW 494483..495652 (-) 1170 WP_013571092.1 radical SAM family heme chaperone HemW -
  FORC77_RS02405 (FORC77_0425) - 495662..496264 (-) 603 WP_011079456.1 XTP/dITP diphosphatase -
  FORC77_RS02410 (FORC77_0426) - 496275..496706 (-) 432 WP_011079457.1 DUF4426 domain-containing protein -
  FORC77_RS02415 (FORC77_0427) yggU 496749..497039 (-) 291 WP_011151199.1 DUF167 family protein YggU -
  FORC77_RS02420 (FORC77_0428) - 497039..497596 (-) 558 WP_013571095.1 YggT family protein -
  FORC77_RS02425 (FORC77_0429) proC 497638..498456 (-) 819 WP_045618603.1 pyrroline-5-carboxylate reductase -
  FORC77_RS02430 (FORC77_0430) - 498491..499201 (-) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC77_RS02435 (FORC77_0431) pilT 499226..500263 (+) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC77_RS02440 (FORC77_0432) pilU 500282..501388 (+) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC77_RS02445 (FORC77_0433) ruvX 501481..501903 (-) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  FORC77_RS02450 (FORC77_0434) - 501925..502488 (-) 564 WP_011079465.1 YqgE/AlgH family protein -
  FORC77_RS02455 (FORC77_0435) gshB 502543..503496 (-) 954 WP_013571098.1 glutathione synthase -
  FORC77_RS02460 (FORC77_0436) rsmE 503511..504242 (-) 732 WP_011079467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38533.19 Da        Isoelectric Point: 6.3696

>NTDB_id=274582 FORC77_RS02435 WP_026050359.1 499226..500263(+) (pilT) [Vibrio vulnificus strain FORC_077]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=274582 FORC77_RS02435 WP_026050359.1 499226..500263(+) (pilT) [Vibrio vulnificus strain FORC_077]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGTTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGT
TTTCGTGTTAACGCCTTCAACCAATCTCGCGGCTGCTCTGCGGTGTTTCGTACTATTCCAACCAATATCCCAACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAAGGCTTGGTGTTGGTCACTGGGCCAACAGGTT
CGGGTAAATCGACCACGTTAGCGGCGATGGTGGATTACATTAACCGCAATTACAACAAACACATCCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACCCACAGTTTTAAAAA
TGCCCTGCGCAGCGCATTGCGTGAGGACCCAGACGTTATCTTGGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTTACCGCGGCAGAAACGGGCCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCATTGCGCGCCGTTATCGCACAGAA
ATTGCTGAAACGCATTGGTGGTGGGCGAGTGGCGTGTCATGAGATCATGATGGCAACGCCCGCCATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAAATGTACTCCATTATCCAAACGGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATGGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.116

100

0.881
  pilT Vibrio cholerae strain A1552

88.116

100

0.881
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.088

92.174

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment