Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MPE_RS18985 Genome accession   NC_008825
Coordinates   3979604..3980647 (+) Length   347 a.a.
NCBI ID   WP_011831333.1    Uniprot ID   W7WBH1
Organism   Methylibium petroleiphilum PM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3974604..3985647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPE_RS18960 (Mpe_A3755) - 3974938..3975651 (+) 714 WP_011831328.1 ABC transporter ATP-binding protein -
  MPE_RS18965 (Mpe_A3756) - 3975700..3976923 (+) 1224 WP_011831329.1 ABC transporter substrate-binding protein -
  MPE_RS18970 (Mpe_A3757) - 3976961..3977848 (+) 888 WP_011831330.1 branched-chain amino acid ABC transporter permease -
  MPE_RS18975 (Mpe_A3758) - 3977845..3978828 (+) 984 WP_011831331.1 branched-chain amino acid ABC transporter permease -
  MPE_RS18980 (Mpe_A3759) - 3978835..3979551 (-) 717 WP_011831332.1 YggS family pyridoxal phosphate-dependent enzyme -
  MPE_RS18985 (Mpe_A3760) pilT 3979604..3980647 (+) 1044 WP_011831333.1 type IV pilus twitching motility protein PilT Machinery gene
  MPE_RS18990 (Mpe_A3761) - 3980685..3981293 (+) 609 WP_011831334.1 cyclic nucleotide-binding domain-containing protein -
  MPE_RS18995 (Mpe_A3762) pilU 3981338..3982474 (+) 1137 WP_011831335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MPE_RS19000 (Mpe_A3763) - 3982569..3983462 (+) 894 WP_011831336.1 NAD(P)-dependent oxidoreductase -
  MPE_RS19005 (Mpe_A3764) - 3983500..3984183 (-) 684 WP_011831337.1 BON domain-containing protein -
  MPE_RS19010 (Mpe_A3765) - 3984180..3984776 (-) 597 WP_036233929.1 phosphoheptose isomerase -
  MPE_RS19015 (Mpe_A3766) - 3984849..3985217 (-) 369 WP_011831339.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38156.83 Da        Isoelectric Point: 7.0668

>NTDB_id=27427 MPE_RS18985 WP_011831333.1 3979604..3980647(+) (pilT) [Methylibium petroleiphilum PM1]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDPLDHKNVHDMVYDIMNDAQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNENEYGHILTVED
PIEFVHESKRCLINQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAAEKDMVRAMLSESLIGVISQTLCKLKDGSGRVAAHEIMLGTPAIRNLIREAKVAQMYSSIQTGNSMGMQTLDQN
LTDLVRRNLITPAEARGKAKLPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=27427 MPE_RS18985 WP_011831333.1 3979604..3980647(+) (pilT) [Methylibium petroleiphilum PM1]
ATGGACATCACCCAACTGCTGGCGTTCTCGGTCAAGAACAAGGCCTCGGACCTGCACTTGTCCTCCGGACTGCCGCCGAT
GATCCGGGTGCACGGCGACGTGCGGCGCATCAACGTGGACCCTCTGGACCACAAGAACGTGCACGACATGGTGTACGACA
TCATGAACGATGCGCAGCGCAAGGCCTACGAGGAGACGCTGGAATGCGACTTCTCGTTCGAGATCCAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAATCAGAACCGCGGCGCGGGCGCGGTATTCCGCACCATCCCGAGCAAGATCCTGACGCTCGA
GCAGTTGAACGCGCCGAAGATCTTCGCCGATCTGGCCCTCAAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACCACGCTCGCCGGCATGGTGAACCACCTCAACGAGAACGAGTACGGCCATATCCTGACCGTCGAGGAT
CCGATCGAGTTCGTGCACGAGTCCAAGCGCTGCCTGATCAACCAGCGCGAGGTCGGACCGCACACGCTGTCCTTCAGCAA
CGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACGCGATCCTGGTCGGCGAAATGCGCGACCTCGAGACCATCCGCCTCG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCAGCAGCGCGGCCAAGACCATCGACCGCGTG
ATCGACGTGTTCCCCGCCGCCGAGAAGGACATGGTGCGCGCGATGCTGTCGGAGTCGCTGATCGGCGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCGCAGATGTACAGCTCGATCCAGACCGGCAACAGCATGGGCATGCAGACACTGGACCAGAAC
CTGACGGACCTGGTGCGCCGCAACCTCATCACGCCGGCAGAGGCACGCGGCAAGGCCAAGCTGCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7WBH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment