Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MPE_RS18995 Genome accession   NC_008825
Coordinates   3981338..3982474 (+) Length   378 a.a.
NCBI ID   WP_011831335.1    Uniprot ID   W7W3I9
Organism   Methylibium petroleiphilum PM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3976338..3987474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPE_RS18970 (Mpe_A3757) - 3976961..3977848 (+) 888 WP_011831330.1 branched-chain amino acid ABC transporter permease -
  MPE_RS18975 (Mpe_A3758) - 3977845..3978828 (+) 984 WP_011831331.1 branched-chain amino acid ABC transporter permease -
  MPE_RS18980 (Mpe_A3759) - 3978835..3979551 (-) 717 WP_011831332.1 YggS family pyridoxal phosphate-dependent enzyme -
  MPE_RS18985 (Mpe_A3760) pilT 3979604..3980647 (+) 1044 WP_011831333.1 type IV pilus twitching motility protein PilT Machinery gene
  MPE_RS18990 (Mpe_A3761) - 3980685..3981293 (+) 609 WP_011831334.1 cyclic nucleotide-binding domain-containing protein -
  MPE_RS18995 (Mpe_A3762) pilU 3981338..3982474 (+) 1137 WP_011831335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MPE_RS19000 (Mpe_A3763) - 3982569..3983462 (+) 894 WP_011831336.1 NAD(P)-dependent oxidoreductase -
  MPE_RS19005 (Mpe_A3764) - 3983500..3984183 (-) 684 WP_011831337.1 BON domain-containing protein -
  MPE_RS19010 (Mpe_A3765) - 3984180..3984776 (-) 597 WP_036233929.1 phosphoheptose isomerase -
  MPE_RS19015 (Mpe_A3766) - 3984849..3985217 (-) 369 WP_011831339.1 YraN family protein -
  MPE_RS19020 (Mpe_A3767) rsmI 3985210..3986142 (+) 933 WP_041930319.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  MPE_RS19025 (Mpe_A3768) - 3986151..3986486 (-) 336 WP_011831341.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41929.09 Da        Isoelectric Point: 6.5038

>NTDB_id=27428 MPE_RS18995 WP_011831335.1 3981338..3982474(+) (pilU) [Methylibium petroleiphilum PM1]
MERDQASKFVNDLLRLLVARNGSDLFLTADFPPAVKVDGRVTKVSPQPLTPQHTMALARSIMNDKQAAEFERTKECNFAI
APTGIGRFRVNAFVQQGCVGLVLRTIPQTLPTIDSLGLPQVLKDVASTKRGLVIFVGATGSGKSTSLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGIDTDDWAPALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLLARQEGRGRVAAIEILLNTPLISDLIFKGEVAEIKEIMKKSRELG
MQTFDQSLFDLYEGQLVTYEDALRNADSVNDLRLQIKLNSNRARNSDLASGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=27428 MPE_RS18995 WP_011831335.1 3981338..3982474(+) (pilU) [Methylibium petroleiphilum PM1]
ATGGAACGCGATCAAGCGTCGAAATTCGTCAACGACCTGCTGCGCCTGCTGGTCGCCCGCAACGGCTCCGATCTGTTCCT
GACGGCCGACTTTCCTCCGGCGGTCAAGGTCGACGGCCGCGTCACCAAGGTATCGCCGCAGCCGCTGACGCCGCAACACA
CGATGGCCCTGGCGCGTTCGATCATGAACGACAAGCAGGCGGCCGAGTTCGAACGCACCAAGGAGTGCAATTTCGCGATC
GCGCCGACCGGCATCGGCCGCTTCCGGGTCAATGCCTTCGTGCAGCAGGGCTGCGTGGGCCTCGTGCTGCGGACGATCCC
GCAGACGCTGCCGACGATCGACTCACTCGGCCTGCCGCAGGTGCTGAAGGACGTCGCCTCGACCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCCGCGATGGTGGACTACCGCAACGAGAACTCCTACGGC
CACATCATCACCATCGAGGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCGTGACGCAGCGTGAGGTCGGCAT
CGATACCGACGACTGGGCCCCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAACACGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGCGCTCGTGTCGCAGCGCCTGCTGGCGCGCCAGGAAGGCCGTGGCCGCGTCGCGGCGATCGAGATCCTGCTGAACA
CGCCGTTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCGAGCTCGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGACCTGTACGAGGGCCAGCTGGTCACCTACGAGGATGCCCTGCGCAACGCCGA
TTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCAACCGGGCGCGCAACTCCGACCTGGCCTCGGGCACCGAGC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7W3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.388

96.825

0.614
  pilU Acinetobacter baylyi ADP1

57.104

96.825

0.553
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

45.768

84.392

0.386
  pilT Acinetobacter baumannii strain A118

45.768

84.392

0.386
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Acinetobacter baylyi ADP1

45.031

85.185

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.283

84.127

0.381
  pilT Vibrio cholerae strain A1552

45.283

84.127

0.381

Multiple sequence alignment