Detailed information NTG0273620

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: C4962_RS04425
• Genome accession: NZ_CP026929
• Coordinates: 911427..912407 (+)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli strain CFS3246
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 906427..917407

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4962_RS04395 (C4962_04770)	yggM	906942..907949 (+)	1008	WP_000745210.1	DUF1202 family protein	-
C4962_RS04400 (C4962_04775)	hemW	908104..909240 (-)	1137	WP_000239943.1	radical SAM family heme chaperone HemW	-
C4962_RS04405 (C4962_04780)	rdgB	909233..909826 (-)	594	WP_001174778.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
C4962_RS04410 (C4962_04785)	yggU	909834..910124 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
C4962_RS04415 (C4962_04790)	yggT	910121..910687 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
C4962_RS04420 (C4962_04795)	yggS	910705..911409 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
C4962_RS04425 (C4962_04800)	pilT	911427..912407 (+)	981	WP_001326494.1	type IV pilus twitching motility protein PilT	Machinery gene
C4962_RS04430 (C4962_04805)	ruvX	912591..913007 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
C4962_RS04435 (C4962_04810)	yqgE	913007..913570 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
C4962_RS04440 (C4962_04815)	gshB	913679..914629 (-)	951	WP_000593273.1	glutathione synthase	-
C4962_RS04445 (C4962_04820)	rsmE	914642..915373 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
C4962_RS04450 (C4962_04825)	endA	915453..916160 (-)	708	WP_000286500.1	deoxyribonuclease I	-
C4962_RS04455 (C4962_04830)	yggI	916255..916752 (-)	498	WP_001333456.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=273620 C4962_RS04425 WP_001326494.1 911427..912407(+) (pilT) [Escherichia coli strain CFS3246]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=273620 C4962_RS04425 WP_001326494.1 911427..912407(+) (pilT) [Escherichia coli strain CFS3246]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362