Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VEIS_RS08235 Genome accession   NC_008786
Coordinates   1882425..1883468 (+) Length   347 a.a.
NCBI ID   WP_011809450.1    Uniprot ID   A1WII6
Organism   Verminephrobacter eiseniae EF01-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1877425..1888468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEIS_RS08215 (Veis_1684) - 1878434..1879120 (+) 687 WP_011809446.1 TetR/AcrR family transcriptional regulator -
  VEIS_RS08220 (Veis_1685) - 1879151..1880419 (+) 1269 WP_011809447.1 branched-chain amino acid ABC transporter substrate-binding protein -
  VEIS_RS08225 (Veis_1686) ltaE 1880567..1881637 (+) 1071 WP_011809448.1 low-specificity L-threonine aldolase -
  VEIS_RS08230 (Veis_1687) - 1881638..1882357 (-) 720 WP_011809449.1 YggS family pyridoxal phosphate-dependent enzyme -
  VEIS_RS08235 (Veis_1688) pilT 1882425..1883468 (+) 1044 WP_011809450.1 type IV pilus twitching motility protein PilT Machinery gene
  VEIS_RS08240 (Veis_1689) - 1883557..1884195 (+) 639 WP_011809451.1 Crp/Fnr family transcriptional regulator -
  VEIS_RS08245 (Veis_1691) pilU 1884538..1885674 (+) 1137 WP_011809453.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VEIS_RS08250 (Veis_1692) - 1885678..1886592 (+) 915 WP_011809454.1 NAD(P)-dependent oxidoreductase -
  VEIS_RS08255 (Veis_1693) - 1886792..1887502 (+) 711 WP_011809455.1 NYN domain-containing protein -
  VEIS_RS31545 - 1887586..1887690 (+) 105 Protein_1760 DNA-binding response regulator -
  VEIS_RS08260 (Veis_1694) - 1887666..1888175 (-) 510 WP_011809456.1 PACE efflux transporter -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38157.07 Da        Isoelectric Point: 6.7744

>NTDB_id=27310 VEIS_RS08235 WP_011809450.1 1882425..1883468(+) (pilT) [Verminephrobacter eiseniae EF01-2]
MDITQLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINIDALDHKAVHSMVYDIMSDSQRKIYEEFLEIDFSFEIEGLAR
FRVNAFTQNRGVAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPSEEKEMIRAMLSESLQAVISQTLCKTRDGAGRVAAHEIMLGTSAIRNLIREAKVPQMYSAIQTGNSVGMQTLDQN
LTDLVKSKIITPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=27310 VEIS_RS08235 WP_011809450.1 1882425..1883468(+) (pilT) [Verminephrobacter eiseniae EF01-2]
GTGGACATTACCCAATTGCTCGCATTCGGCGTCAAGAACAAGGCGTCGGATTTGCACCTTTCTGCTGGTCTGCCGCCGAT
GATCCGGGTCCATGGCGACGTGCGGCGCATCAACATCGATGCGCTCGACCACAAGGCGGTGCATTCCATGGTGTACGACA
TCATGAGCGACTCCCAACGCAAAATCTATGAGGAATTTCTGGAGATCGACTTCTCGTTTGAAATCGAAGGCTTGGCGCGC
TTTCGCGTCAACGCCTTCACCCAGAACCGCGGCGTGGCCGCCGTGTTCCGCACGATTCCGAGCAAAATCCTGACGCTCGA
GCAACTCAACGCCCCGAGGATCTTCGCCGACCTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCAATTTCCTGAACGAAACCGAGTACGGCCATATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTAGGCCCGATGACGCTGTCGTTCTCCGC
CGCGCTCAAATCGGCCTTGCGCGAAGACCCCGACGCCATCCTGGTCGGCGAAATGCGTGATCTGGAAACCATCCGCCTGG
CGATGACGGCCGCTGAAACCGGCCACTTGGTATTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATAGACCGGATC
ATCGACGTGTTCCCGTCCGAAGAAAAAGAAATGATCCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAAAC
CCTGTGCAAGACGCGCGACGGCGCGGGCCGCGTGGCGGCGCATGAAATCATGCTCGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGCCGCAGATGTACTCGGCGATACAGACCGGCAACAGCGTGGGCATGCAAACCCTGGATCAGAAC
CTGACCGACCTGGTAAAGAGCAAAATCATCACCCCAGCCGAAGCGCGCAGCAAAGCCAAGATTCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1WII6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Acinetobacter nosocomialis M2

74.203

99.424

0.738
  pilT Acinetobacter baumannii D1279779

74.203

99.424

0.738
  pilT Acinetobacter baumannii strain A118

74.203

99.424

0.738
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Neisseria meningitidis 8013

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.982

98.271

0.648
  pilT Vibrio cholerae strain A1552

65.982

98.271

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392

Multiple sequence alignment