Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VEIS_RS08245 Genome accession   NC_008786
Coordinates   1884538..1885674 (+) Length   378 a.a.
NCBI ID   WP_011809453.1    Uniprot ID   A1WII9
Organism   Verminephrobacter eiseniae EF01-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1879538..1890674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEIS_RS08225 (Veis_1686) ltaE 1880567..1881637 (+) 1071 WP_011809448.1 low-specificity L-threonine aldolase -
  VEIS_RS08230 (Veis_1687) - 1881638..1882357 (-) 720 WP_011809449.1 YggS family pyridoxal phosphate-dependent enzyme -
  VEIS_RS08235 (Veis_1688) pilT 1882425..1883468 (+) 1044 WP_011809450.1 type IV pilus twitching motility protein PilT Machinery gene
  VEIS_RS08240 (Veis_1689) - 1883557..1884195 (+) 639 WP_011809451.1 Crp/Fnr family transcriptional regulator -
  VEIS_RS08245 (Veis_1691) pilU 1884538..1885674 (+) 1137 WP_011809453.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VEIS_RS08250 (Veis_1692) - 1885678..1886592 (+) 915 WP_011809454.1 NAD(P)-dependent oxidoreductase -
  VEIS_RS08255 (Veis_1693) - 1886792..1887502 (+) 711 WP_011809455.1 NYN domain-containing protein -
  VEIS_RS31545 - 1887586..1887690 (+) 105 Protein_1760 DNA-binding response regulator -
  VEIS_RS08260 (Veis_1694) - 1887666..1888175 (-) 510 WP_011809456.1 PACE efflux transporter -
  VEIS_RS08265 (Veis_1695) - 1888547..1889515 (-) 969 WP_011809457.1 GMP reductase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41923.23 Da        Isoelectric Point: 7.2382

>NTDB_id=27311 VEIS_RS08245 WP_011809453.1 1884538..1885674(+) (pilU) [Verminephrobacter eiseniae EF01-2]
MDRDQASKFINDLLRLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLSPSHTQTLARAIMSDKQVSDFERTKECNFAI
SPAGIGRFRVNTFIQQGKVGMVLRTIPLTPPTIDSLKLPQVLKEVSMHKRGLCILVGATGSGKTTTLAALVDWRNENSFG
HIVTVEDPVEFVHSHKNCVVTQREVGLDTDSWELALKNALRQAPDVVLMGEIRDRETMAHAITFSETGHLCLATLHANSA
NQALDRIIHFFPEDSRIQMLMDLSLNLRALISQRLIPKQDGRGRIAAVEVLLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDAYDAYAISYEDALRNADSVNDLRLQIKLNSQRAKSPDLAAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=27311 VEIS_RS08245 WP_011809453.1 1884538..1885674(+) (pilU) [Verminephrobacter eiseniae EF01-2]
ATGGACCGCGACCAGGCCAGCAAATTCATCAATGACCTGTTGCGGCTGATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCAGAATTTCCGCCGGCCATCAAGGTCGATGGCAAGGTGACCAAGGTCTCGCCCCAGCCGCTGTCCCCGAGCCACA
CGCAGACGCTGGCCCGCGCCATCATGAGCGACAAACAGGTGTCCGACTTCGAGCGCACCAAAGAATGCAACTTCGCCATC
TCGCCCGCCGGCATCGGCCGCTTTCGGGTCAACACCTTCATACAGCAGGGCAAGGTCGGCATGGTGCTGCGCACGATTCC
GCTGACGCCGCCGACCATCGACAGCCTGAAACTGCCGCAGGTGCTCAAGGAAGTGTCCATGCACAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGACCACTACGCTGGCAGCGCTCGTCGACTGGCGCAACGAAAACTCGTTCGGC
CACATCGTCACGGTGGAAGACCCGGTCGAATTCGTGCACTCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGTCT
GGACACCGACAGTTGGGAACTTGCGCTCAAAAACGCGCTGCGCCAAGCCCCCGACGTGGTCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGCGCACGCGATCACCTTCTCCGAAACCGGCCATCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGGATCATCCACTTCTTCCCCGAAGATTCCCGCATCCAGATGCTGATGGACCTGTCACTGAACCT
GCGCGCCCTGATATCGCAGCGCCTGATCCCGAAACAGGACGGCAGGGGCCGCATCGCTGCGGTCGAGGTGCTGCTCAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCTCGGAGATCAAAGAAATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACGCCTACGATGCCTACGCGATCAGCTACGAAGACGCGCTGCGCAACGCCGA
TTCGGTCAATGACCTGCGCCTGCAAATCAAACTCAACAGCCAACGCGCCAAGTCGCCCGACCTGGCCGCTGGCACCGAGC
ATTTAGGGATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1WII9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.891

92.328

0.571
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

53.161

92.063

0.489
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

89.153

0.381
  pilT Legionella pneumophila strain ERS1305867

41.742

88.095

0.368
  pilT Legionella pneumophila strain Lp02

41.742

88.095

0.368
  pilT Acinetobacter baumannii strain A118

43.26

84.392

0.365
  pilT Acinetobacter nosocomialis M2

43.26

84.392

0.365
  pilT Acinetobacter baumannii D1279779

43.26

84.392

0.365

Multiple sequence alignment