Detailed information NTG0273061

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: C5F71_RS10880
• Genome accession: NZ_CP026881
• Coordinates: 2255214..2256248 (-)
• Length: 344 a.a.
• NCBI ID: WP_104911126.1
• Uniprot ID: -
• Organism: Pseudomonas sp. LG1D9
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2250214..2261248

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C5F71_RS10845	ruvX	2250304..2250741 (+)	438	WP_005792259.1	Holliday junction resolvase RuvX	-
C5F71_RS10850	pyrR	2250824..2251330 (+)	507	WP_029300467.1	bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR	-
C5F71_RS10855	-	2251355..2252359 (+)	1005	WP_029300469.1	aspartate carbamoyltransferase catalytic subunit	-
C5F71_RS10860	-	2252356..2253627 (+)	1272	WP_104911123.1	dihydroorotase	-
C5F71_RS10865	-	2253772..2254200 (-)	429	WP_104911124.1	TM2 domain-containing protein	-
C5F71_RS10870	-	2254450..2255085 (+)	636	WP_029300475.1	C40 family peptidase	-
C5F71_RS10880	pilT	2255214..2256248 (-)	1035	WP_104911126.1	type IV pilus twitching motility protein PilT	Machinery gene
C5F71_RS10885	-	2256306..2256992 (+)	687	WP_029300479.1	YggS family pyridoxal phosphate-dependent enzyme	-
C5F71_RS10890	proC	2257026..2257844 (+)	819	WP_029300480.1	pyrroline-5-carboxylate reductase	-
C5F71_RS10895	-	2257854..2258444 (+)	591	WP_029300481.1	YggT family protein	-
C5F71_RS10900	-	2258607..2259746 (+)	1140	WP_092231136.1	homoserine O-acetyltransferase	-
C5F71_RS10905	metW	2259754..2260374 (+)	621	WP_029300485.1	methionine biosynthesis protein MetW	-
C5F71_RS10910	-	2260396..2260830 (+)	435	WP_029300487.1	DUF4426 domain-containing protein	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 37428.03 Da
• Isoelectric Point: 6.8391

>NTDB_id=273061 C5F71_RS10880 WP_104911126.1 2255214..2256248(-) (pilT) [Pseudomonas sp. LG1D9]
MDITELLTACVSRGASDLHLSAGLAPRMRVDGEVVPLDWPPLSATQVADLLSPIMNKHQQKDFETSLETDFSFELPGVAR
FRANVFQQHRGMGAVFRAIPSRVQSLESLGLGDVFRRIAELPRGLVLVTGATGSGKSTTLAAMIDYLNNTRQQHILTLED
PIEFVHEPRTALISQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPSGEKAMVRSMLSESLQAVVSQMLLKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQLYSAIQTGGALGMKTLDMSL
KGLVSQGLVSREEAREQARVPAEL

Nucleotide

• Length: 1035 bp

>NTDB_id=273061 C5F71_RS10880 WP_104911126.1 2255214..2256248(-) (pilT) [Pseudomonas sp. LG1D9]
ATGGACATCACTGAACTGCTGACCGCCTGCGTCAGCCGGGGCGCCTCCGACCTGCATCTGTCGGCAGGGTTGGCGCCGAG
GATGCGAGTCGATGGCGAGGTTGTGCCACTCGACTGGCCCCCATTGAGTGCTACGCAAGTAGCGGACTTACTCAGCCCGA
TCATGAATAAACACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTTCCTTCGAGTTGCCCGGTGTGGCGCGC
TTTCGCGCCAATGTGTTCCAGCAGCACCGAGGGATGGGCGCGGTGTTTCGCGCGATTCCCTCCCGGGTACAGAGCCTGGA
AAGCCTGGGGCTGGGCGACGTGTTCCGGCGCATCGCCGAACTGCCTCGGGGGCTGGTGCTGGTGACCGGGGCCACGGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTCAACAACACCCGCCAGCAGCACATCCTGACCCTTGAAGAC
CCTATCGAATTTGTCCATGAACCGCGCACGGCGCTGATCAGCCAGCGCCAGGTGCATCGCGATACCCACAGTTTCTCCGC
CGCCCTGCGTTCTGCGCTGCGCGAAGACCCGGATGTGATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACGGCGGCTGAAACCGGGCACCTGGTGTTTGGCACCCTGCACACCAGTTCGGCGGCAAAGACCGTGGACCGGCTG
GTGGATGTGTTCCCCTCGGGGGAAAAGGCCATGGTCCGCTCGATGCTCTCGGAGTCGCTGCAGGCGGTGGTTTCCCAGAT
GTTGCTGAAAAAAGTCGGCGGCGGGCGCGTGGCGGCCCATGAAATCATGCTCGGTACACCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGTTGTACTCGGCGATCCAGACGGGAGGGGCGTTGGGGATGAAGACGTTGGATATGAGCTTG
AAGGGGCTGGTGAGTCAGGGGCTGGTCAGCCGCGAAGAGGCCCGGGAGCAGGCACGGGTGCCCGCAGAGTTATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas aeruginosa PAK	74.487	99.128	0.738
pilT	Pseudomonas stutzeri DSM 10701	73.021	99.128	0.724
pilT	Acinetobacter nosocomialis M2	70.088	99.128	0.695
pilT	Acinetobacter baumannii D1279779	69.795	99.128	0.692
pilT	Acinetobacter baumannii strain A118	69.795	99.128	0.692
pilT	Acinetobacter baylyi ADP1	69.501	99.128	0.689
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	64.688	97.965	0.634
pilT	Vibrio cholerae strain A1552	64.688	97.965	0.634
pilT	Legionella pneumophila strain Lp02	64.201	98.256	0.631
pilT	Legionella pneumophila strain ERS1305867	64.201	98.256	0.631
pilT	Neisseria meningitidis 8013	62.941	98.837	0.622
pilT	Neisseria gonorrhoeae MS11	62.647	98.837	0.619
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	50.725	100	0.509
pilU	Pseudomonas stutzeri DSM 10701	40.299	97.384	0.392
pilU	Vibrio cholerae strain A1552	40	97.384	0.39
pilU	Acinetobacter baylyi ADP1	37.572	100	0.378
pilB	Acinetobacter baumannii D1279779	32.552	100	0.363