Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1P57_RS21060 Genome accession   NZ_CP026846
Coordinates   4177756..4178736 (-) Length   326 a.a.
NCBI ID   WP_001055629.1    Uniprot ID   A0A398TTF2
Organism   Shigella boydii strain 59-2708     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4172756..4183736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1P57_RS21030 - 4173419..4173916 (+) 498 WP_078167026.1 SprT family zinc-dependent metalloprotease -
  C1P57_RS21035 endA 4174011..4174718 (+) 708 WP_000286500.1 deoxyribonuclease I -
  C1P57_RS21040 rsmE 4174798..4175529 (+) 732 WP_078167025.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1P57_RS21045 gshB 4175542..4176492 (+) 951 WP_000593264.1 glutathione synthase -
  C1P57_RS21050 - 4176601..4177164 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  C1P57_RS21055 ruvX 4177164..4177580 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C1P57_RS21060 pilT 4177756..4178736 (-) 981 WP_001055629.1 type IV pilus twitching motility protein PilT Machinery gene
  C1P57_RS21065 yggS 4178754..4179458 (+) 705 WP_078167024.1 pyridoxal phosphate homeostasis protein -
  C1P57_RS21070 yggT 4179476..4180042 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  C1P57_RS21075 yggU 4180039..4180329 (+) 291 WP_078167023.1 DUF167 family protein YggU -
  C1P57_RS21080 - 4180337..4180930 (+) 594 WP_134795883.1 XTP/dITP diphosphatase -
  C1P57_RS21085 hemW 4180923..4182059 (+) 1137 WP_078167022.1 radical SAM family heme chaperone HemW -
  C1P57_RS21090 - 4182367..4183352 (+) 986 Protein_4071 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35884.09 Da        Isoelectric Point: 5.9959

>NTDB_id=272959 C1P57_RS21060 WP_001055629.1 4177756..4178736(-) (pilT) [Shigella boydii strain 59-2708]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVGKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272959 C1P57_RS21060 WP_001055629.1 4177756..4178736(-) (pilT) [Shigella boydii strain 59-2708]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGGTAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A398TTF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment