Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1P56_RS22335 Genome accession   NZ_CP026836
Coordinates   4362659..4363639 (+) Length   326 a.a.
NCBI ID   WP_001055629.1    Uniprot ID   A0A398TTF2
Organism   Shigella boydii strain ATCC 49812     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4357659..4368639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1P56_RS22305 - 4358085..4359105 (-) 1021 Protein_4346 IS630 family transposase -
  C1P56_RS22310 hemW 4359336..4360472 (-) 1137 WP_000239925.1 radical SAM family heme chaperone HemW -
  C1P56_RS22315 - 4360465..4361058 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  C1P56_RS22320 yggU 4361066..4361356 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  C1P56_RS22325 yggT 4361353..4361919 (-) 567 WP_096843180.1 osmotic shock tolerance protein YggT -
  C1P56_RS22330 yggS 4361937..4362641 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C1P56_RS22335 pilT 4362659..4363639 (+) 981 WP_001055629.1 type IV pilus twitching motility protein PilT Machinery gene
  C1P56_RS22340 ruvX 4363815..4364231 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C1P56_RS22345 - 4364231..4364794 (-) 564 WP_119172541.1 YqgE/AlgH family protein -
  C1P56_RS22350 gshB 4364903..4365853 (-) 951 WP_134796636.1 glutathione synthase -
  C1P56_RS22355 rsmE 4365866..4366597 (-) 732 WP_004972872.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1P56_RS22360 endA 4366677..4367384 (-) 708 WP_134796638.1 deoxyribonuclease I -
  C1P56_RS22365 - 4367479..4367976 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35884.09 Da        Isoelectric Point: 5.9959

>NTDB_id=272911 C1P56_RS22335 WP_001055629.1 4362659..4363639(+) (pilT) [Shigella boydii strain ATCC 49812]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVGKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272911 C1P56_RS22335 WP_001055629.1 4362659..4363639(+) (pilT) [Shigella boydii strain ATCC 49812]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGGTAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A398TTF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment