Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C5B80_RS01425 Genome accession   NZ_CP026795
Coordinates   264753..265733 (+) Length   326 a.a.
NCBI ID   WP_012421359.1    Uniprot ID   B2U0X0
Organism   Shigella boydii strain ATCC BAA-1247     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259753..270733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5B80_RS01395 - 260268..261275 (+) 1008 WP_000745217.1 DUF1202 family protein -
  C5B80_RS01400 hemW 261430..262566 (-) 1137 WP_000239922.1 radical SAM family heme chaperone HemW -
  C5B80_RS01405 - 262559..263152 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  C5B80_RS01410 yggU 263160..263450 (-) 291 WP_000994920.1 DUF167 family protein YggU -
  C5B80_RS01415 yggT 263447..264013 (-) 567 WP_001094828.1 osmotic shock tolerance protein YggT -
  C5B80_RS01420 yggS 264031..264735 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C5B80_RS01425 pilT 264753..265733 (+) 981 WP_012421359.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C5B80_RS01430 ruvX 265909..266325 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C5B80_RS01435 - 266325..266888 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  C5B80_RS01440 gshB 266997..267947 (-) 951 WP_000593283.1 glutathione synthase -
  C5B80_RS01445 rsmE 267960..268691 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C5B80_RS01450 endA 268771..269478 (-) 708 WP_000286500.1 deoxyribonuclease I -
  C5B80_RS01455 - 269573..270070 (-) 498 WP_012421484.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35934.19 Da        Isoelectric Point: 5.7654

>NTDB_id=272591 C5B80_RS01425 WP_012421359.1 264753..265733(+) (pilT) [Shigella boydii strain ATCC BAA-1247]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEPIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLLQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272591 C5B80_RS01425 WP_012421359.1 264753..265733(+) (pilT) [Shigella boydii strain ATCC BAA-1247]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGCCAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTTACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2U0X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368
  pilB Legionella pneumophila strain ERS1305867

31.383

100

0.362

Multiple sequence alignment