Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1P58_RS09075 Genome accession   NZ_CP026766
Coordinates   1743908..1744888 (+) Length   326 a.a.
NCBI ID   WP_073691825.1    Uniprot ID   -
Organism   Shigella boydii strain 59-248     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1738908..1749888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1P58_RS09045 - 1739423..1740430 (+) 1008 WP_073691823.1 DUF1202 family protein -
  C1P58_RS09050 hemW 1740585..1741721 (-) 1137 WP_134802327.1 radical SAM family heme chaperone HemW -
  C1P58_RS09055 - 1741714..1742307 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  C1P58_RS09060 yggU 1742315..1742605 (-) 291 WP_000994920.1 DUF167 family protein YggU -
  C1P58_RS09065 yggT 1742602..1743168 (-) 567 WP_001094829.1 osmotic shock tolerance protein YggT -
  C1P58_RS09070 yggS 1743186..1743890 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C1P58_RS09075 pilT 1743908..1744888 (+) 981 WP_073691825.1 type IV pilus twitching motility protein PilT Machinery gene
  C1P58_RS09080 ruvX 1745064..1745480 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C1P58_RS09085 - 1745480..1746043 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  C1P58_RS09090 gshB 1746152..1747102 (-) 951 WP_000593283.1 glutathione synthase -
  C1P58_RS09095 rsmE 1747115..1747846 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1P58_RS09100 endA 1747926..1748633 (-) 708 WP_000286500.1 deoxyribonuclease I -
  C1P58_RS09105 - 1748728..1749225 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35924.15 Da        Isoelectric Point: 5.7654

>NTDB_id=272351 C1P58_RS09075 WP_073691825.1 1743908..1744888(+) (pilT) [Shigella boydii strain 59-248]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKPHQLLHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272351 C1P58_RS09075 WP_073691825.1 1743908..1744888(+) (pilT) [Shigella boydii strain 59-248]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AACCCCACCAGTTACTGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365

Multiple sequence alignment