Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1P50_RS14945 Genome accession   NZ_CP026762
Coordinates   2774097..2775077 (-) Length   326 a.a.
NCBI ID   WP_012421359.1    Uniprot ID   B2U0X0
Organism   Shigella boydii strain NCTC 9850     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2769097..2780077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1P50_RS14915 - 2769760..2770257 (+) 498 WP_012421484.1 SprT family zinc-dependent metalloprotease -
  C1P50_RS14920 endA 2770352..2771059 (+) 708 WP_000286500.1 deoxyribonuclease I -
  C1P50_RS14925 rsmE 2771139..2771870 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1P50_RS14930 gshB 2771883..2772833 (+) 951 WP_000593283.1 glutathione synthase -
  C1P50_RS14935 - 2772942..2773505 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  C1P50_RS14940 ruvX 2773505..2773921 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  C1P50_RS14945 pilT 2774097..2775077 (-) 981 WP_012421359.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1P50_RS14950 yggS 2775095..2775799 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C1P50_RS14955 yggT 2775817..2776383 (+) 567 WP_001094828.1 osmotic shock tolerance protein YggT -
  C1P50_RS14960 yggU 2776380..2776670 (+) 291 WP_000994920.1 DUF167 family protein YggU -
  C1P50_RS14965 - 2776678..2777271 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  C1P50_RS14970 hemW 2777264..2778400 (+) 1137 WP_000239922.1 radical SAM family heme chaperone HemW -
  C1P50_RS14975 - 2778555..2779562 (-) 1008 WP_000745217.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35934.19 Da        Isoelectric Point: 5.7654

>NTDB_id=272315 C1P50_RS14945 WP_012421359.1 2774097..2775077(-) (pilT) [Shigella boydii strain NCTC 9850]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEPIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLLQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=272315 C1P50_RS14945 WP_012421359.1 2774097..2775077(-) (pilT) [Shigella boydii strain NCTC 9850]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGCCAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTTACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2U0X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368
  pilB Legionella pneumophila strain ERS1305867

31.383

100

0.362

Multiple sequence alignment