Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4Q28_RS25390 Genome accession   NZ_CP026676
Coordinates   5457499..5458497 (-) Length   332 a.a.
NCBI ID   WP_104882286.1    Uniprot ID   -
Organism   Pseudomonas sp. SWI6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5452499..5463497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4Q28_RS25370 (C4Q28_25370) argS 5453731..5455467 (+) 1737 WP_023378217.1 arginine--tRNA ligase -
  C4Q28_RS25375 (C4Q28_25375) - 5455469..5456167 (+) 699 WP_023378216.1 SPOR domain-containing protein -
  C4Q28_RS25380 (C4Q28_25380) - 5456209..5456628 (-) 420 WP_023378215.1 TM2 domain-containing protein -
  C4Q28_RS25385 (C4Q28_25385) - 5456797..5457423 (+) 627 WP_104882285.1 C40 family peptidase -
  C4Q28_RS25390 (C4Q28_25390) pilT 5457499..5458497 (-) 999 WP_104882286.1 type IV pilus twitching motility protein PilT Machinery gene
  C4Q28_RS25395 (C4Q28_25395) - 5458563..5459249 (+) 687 WP_023378212.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4Q28_RS25400 (C4Q28_25400) proC 5459288..5460106 (+) 819 WP_023378211.1 pyrroline-5-carboxylate reductase -
  C4Q28_RS25405 (C4Q28_25405) - 5460117..5460707 (+) 591 WP_023378210.1 YggT family protein -
  C4Q28_RS25410 (C4Q28_25410) - 5460797..5461936 (+) 1140 WP_027907907.1 homoserine O-acetyltransferase -
  C4Q28_RS25415 (C4Q28_25415) metW 5461944..5462564 (+) 621 WP_023378208.1 methionine biosynthesis protein MetW -
  C4Q28_RS25420 (C4Q28_25420) - 5462586..5463020 (+) 435 WP_023378207.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35727.33 Da        Isoelectric Point: 6.6988

>NTDB_id=271633 C4Q28_RS25390 WP_104882286.1 5457499..5458497(-) (pilT) [Pseudomonas sp. SWI6]
MDVTDLLARAMAAGASDLHLAAGDVPLIRLDGELQRLDLPPLSATALHDGLAPLLAEAQCRQWAQGDELDLALDLPSLGR
IRLNLFRQSNGPAVAMRIIAQRITTLEALGLTEVFRAFAQHTDGLVLVGGPTGSGKSSTLAALVDQLNRERSLHIITLED
PIEVIHSCQRSLVNQREIGRHCRDFAQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAINSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRRGGGRVAAREVLVATPAVRNLIREGRAAQLQSVMQSGAAAGMKTMEGAM
EALRAEGLIDAL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=271633 C4Q28_RS25390 WP_104882286.1 5457499..5458497(-) (pilT) [Pseudomonas sp. SWI6]
ATGGACGTGACTGACCTCTTGGCCCGGGCGATGGCTGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGACGTACCGCT
GATTCGCCTGGATGGCGAGCTGCAACGCCTGGATCTGCCGCCGTTGTCGGCGACGGCGCTGCACGACGGGCTGGCGCCTT
TGCTGGCCGAAGCGCAGTGTCGCCAGTGGGCGCAAGGTGATGAGCTGGACCTGGCGCTCGATCTCCCCTCGCTTGGGCGT
ATCCGCTTGAATCTGTTTCGCCAGTCGAATGGGCCTGCGGTGGCCATGCGGATCATTGCGCAACGAATCACCACACTCGA
AGCGTTAGGCCTGACAGAAGTGTTTCGAGCTTTTGCCCAACATACCGATGGCCTCGTCCTGGTAGGTGGGCCGACCGGTA
GCGGGAAGTCCAGCACGCTGGCCGCGCTGGTCGACCAGCTCAATCGCGAGCGCTCGCTGCACATCATCACATTGGAAGAC
CCTATCGAAGTTATCCACAGTTGCCAGCGCAGCCTGGTCAACCAGCGCGAGATCGGACGCCACTGTCGTGACTTCGCCCA
GGGGTTGCGCAGTGCACTGCGCCAAGACCCGGATGTCATCGTGATCGGCGAACTGAGGGATCTCGAAACGATTCGTCTGG
CGCTCCGGGCTGCCGAGACGGGGCATCTGGTGCTGGCGACCGTCCATACCCGGTCGGCGATCAACAGCATCGACCGTCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAGCCGTTGGTCAGGGCCATGCTGGCCGAATCGCTGCGCTTGGTGGTAGCGCAGGT
GCTGGTGAAACGAAGAGGTGGCGGCCGGGTGGCGGCGCGGGAAGTGTTGGTGGCGACGCCCGCTGTGCGTAACCTGATCC
GAGAGGGGCGAGCGGCGCAGTTGCAGTCCGTGATGCAGTCGGGGGCGGCGGCGGGGATGAAGACGATGGAAGGAGCGATG
GAGGCGTTGAGGGCAGAAGGGCTGATCGACGCGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.755

99.699

0.506
  pilT Vibrio cholerae strain A1552

50.755

99.699

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter nosocomialis M2

49.848

99.096

0.494
  pilT Neisseria meningitidis 8013

48.485

99.398

0.482
  pilT Legionella pneumophila strain Lp02

48.632

99.096

0.482
  pilT Legionella pneumophila strain ERS1305867

48.632

99.096

0.482
  pilT Neisseria gonorrhoeae MS11

48.182

99.398

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.061

99.398

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377
  pilU Vibrio cholerae strain A1552

37.346

97.59

0.364
  pilF Thermus thermophilus HB27

31.25

100

0.361

Multiple sequence alignment