Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SAMA_RS12925 Genome accession   NC_008700
Coordinates   2979192..2980229 (-) Length   345 a.a.
NCBI ID   WP_011760586.1    Uniprot ID   A1S8H3
Organism   Shewanella amazonensis SB2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2974192..2985229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAMA_RS12890 (Sama_2469) - 2974744..2975304 (-) 561 WP_011760579.1 DUF4136 domain-containing protein -
  SAMA_RS12895 (Sama_2470) - 2975496..2975825 (+) 330 WP_011760580.1 hypothetical protein -
  SAMA_RS12900 (Sama_2471) yciH 2975939..2976268 (+) 330 WP_011760581.1 stress response translation initiation inhibitor YciH -
  SAMA_RS12905 (Sama_2472) - 2976277..2976840 (+) 564 WP_041410422.1 YqgE/AlgH family protein -
  SAMA_RS12910 (Sama_2473) ruvX 2976840..2977259 (+) 420 WP_011760583.1 Holliday junction resolvase RuvX -
  SAMA_RS12915 (Sama_2474) - 2977327..2977869 (-) 543 WP_011760584.1 glutathione peroxidase -
  SAMA_RS12920 (Sama_2475) pilU 2978070..2979182 (-) 1113 WP_011760585.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SAMA_RS12925 (Sama_2476) pilT 2979192..2980229 (-) 1038 WP_011760586.1 type IV pilus twitching motility protein PilT Machinery gene
  SAMA_RS12930 (Sama_2477) - 2980265..2980972 (+) 708 WP_011760587.1 YggS family pyridoxal phosphate-dependent enzyme -
  SAMA_RS12935 (Sama_2478) proC 2981064..2981879 (+) 816 WP_011760588.1 pyrroline-5-carboxylate reductase -
  SAMA_RS12940 (Sama_2479) - 2981925..2982473 (+) 549 WP_011760589.1 YggT family protein -
  SAMA_RS12945 (Sama_2480) yggU 2982473..2982760 (+) 288 WP_011760590.1 DUF167 family protein YggU -
  SAMA_RS12950 (Sama_2481) - 2982843..2983277 (+) 435 WP_011760591.1 DUF4426 domain-containing protein -
  SAMA_RS12955 (Sama_2482) rdgB 2983409..2984005 (+) 597 WP_011760592.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SAMA_RS12960 (Sama_2483) hemW 2984015..2985154 (+) 1140 WP_011760593.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38277.95 Da        Isoelectric Point: 6.9095

>NTDB_id=27072 SAMA_RS12925 WP_011760586.1 2979192..2980229(-) (pilT) [Shewanella amazonensis SB2B]
MEITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHAGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=27072 SAMA_RS12925 WP_011760586.1 2979192..2980229(-) (pilT) [Shewanella amazonensis SB2B]
ATGGAAATCACAGAGTTACTGGCGTTTAGTGTAAAGCACAAGGCCTCGGATCTACACCTGTCTGCCGGTGTTCCCCCCAT
GATCCGGGTGGACGGTGAAGTGCGTAAAATCAATCTGCCTGCACTCGATCATGCCGGCGTACACTCCTTGGTGTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCACCTGGAAATCGACTTTTCCTTTGAAGTGCCCAATCTTGCCCGT
TTTCGTGTAAACGCCTTTAACCAGTCCCGCGGCGCTGCTGCGGTGTTTCGTACCATTCCCAGCGAAATTCTGTCGCTGGA
GCAACTGGGCGCCCCGGACATCTTCAAAAAAATCGCCAGTTTTCCCCGTGGTCTGGTGCTGGTGACCGGCCCAACCGGCT
CAGGTAAAAGTACCACCCTGGCGGCCATGATTGACTATGTGAATGAAAACCGCCACGACCATATTCTGACCATTGAAGAC
CCTATTGAATTCGTGCACCAGAATAAGCAATGTCTTATTAACCAGCGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
TGCCCTTCGCAGCGCGCTGCGTGAAGACCCCGACGTTATTCTGGTGGGTGAGATGCGTGACCTTGAAACTATCCGACTGG
CGATGACCGCGGCAGAAACCGGCCACCTGGTTTTCGGCACCCTGCACACCACCTCGGCAGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCCGCCGGTGAAAAGGACATGGTGCGTACCATGCTGTCTGAATCTTTGCAGGCAGTAATTTCCCAGAC
GCTGATTAAAAAGATTGGCGGCGGCCGGGTTGCGGCCCACGAAATTATGATGGGTACCCCTGCGATTCGTAACCTTATCC
GTGAGGACAAGGTGGCGCAGATGTACTCTGCCATTCAAACCGGTATGGCCCACGGCATGCAAACCCTCGAGCAGTGTCTG
CAGAATCTGGTGAACCGTGGCCTCATCACTCGTGAGGACGCCATGGCCAAGAGCTCCAACAAACAGGCAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1S8H3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.702

99.13

0.8
  pilT Acinetobacter baumannii D1279779

80.702

99.13

0.8
  pilT Acinetobacter baumannii strain A118

80.702

99.13

0.8
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Legionella pneumophila strain Lp02

75.953

98.841

0.751
  pilT Legionella pneumophila strain ERS1305867

75.953

98.841

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

43.149

99.42

0.429
  pilU Vibrio cholerae strain A1552

41.691

99.42

0.414
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394

Multiple sequence alignment