Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SAMA_RS12920 Genome accession   NC_008700
Coordinates   2978070..2979182 (-) Length   370 a.a.
NCBI ID   WP_011760585.1    Uniprot ID   A1S8H2
Organism   Shewanella amazonensis SB2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973070..2984182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAMA_RS12880 (Sama_2467) - 2973368..2973877 (-) 510 WP_011760578.1 DUF924 family protein -
  SAMA_RS12890 (Sama_2469) - 2974744..2975304 (-) 561 WP_011760579.1 DUF4136 domain-containing protein -
  SAMA_RS12895 (Sama_2470) - 2975496..2975825 (+) 330 WP_011760580.1 hypothetical protein -
  SAMA_RS12900 (Sama_2471) yciH 2975939..2976268 (+) 330 WP_011760581.1 stress response translation initiation inhibitor YciH -
  SAMA_RS12905 (Sama_2472) - 2976277..2976840 (+) 564 WP_041410422.1 YqgE/AlgH family protein -
  SAMA_RS12910 (Sama_2473) ruvX 2976840..2977259 (+) 420 WP_011760583.1 Holliday junction resolvase RuvX -
  SAMA_RS12915 (Sama_2474) - 2977327..2977869 (-) 543 WP_011760584.1 glutathione peroxidase -
  SAMA_RS12920 (Sama_2475) pilU 2978070..2979182 (-) 1113 WP_011760585.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SAMA_RS12925 (Sama_2476) pilT 2979192..2980229 (-) 1038 WP_011760586.1 type IV pilus twitching motility protein PilT Machinery gene
  SAMA_RS12930 (Sama_2477) - 2980265..2980972 (+) 708 WP_011760587.1 YggS family pyridoxal phosphate-dependent enzyme -
  SAMA_RS12935 (Sama_2478) proC 2981064..2981879 (+) 816 WP_011760588.1 pyrroline-5-carboxylate reductase -
  SAMA_RS12940 (Sama_2479) - 2981925..2982473 (+) 549 WP_011760589.1 YggT family protein -
  SAMA_RS12945 (Sama_2480) yggU 2982473..2982760 (+) 288 WP_011760590.1 DUF167 family protein YggU -
  SAMA_RS12950 (Sama_2481) - 2982843..2983277 (+) 435 WP_011760591.1 DUF4426 domain-containing protein -
  SAMA_RS12955 (Sama_2482) rdgB 2983409..2984005 (+) 597 WP_011760592.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41117.13 Da        Isoelectric Point: 6.0935

>NTDB_id=27071 SAMA_RS12920 WP_011760585.1 2978070..2979182(-) (pilU) [Shewanella amazonensis SB2B]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFSPAQSLDFVESLMSDAQKKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEMEDLKLPPVLKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHERGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFKLYQEGEITYADALHHADSPNDLRLMIKLAGSESTGSGFLEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=27071 SAMA_RS12920 WP_011760585.1 2978070..2979182(-) (pilU) [Shewanella amazonensis SB2B]
ATGGATGTACGTCCCTTTCTGAAGGTGATGGTCGAGCGCAAAGCCTCGGATTTGTTCATCACCGCGGGTTTTCCTCCCAG
CGCCAAAATCGACGGTGAGCTGCGGCCACTGGCGGAAAATGCCTTCAGCCCTGCCCAATCACTGGACTTTGTTGAGTCCC
TGATGTCAGATGCGCAAAAGAAAGAGTTCCACGAGAGCCGTGAGTGTAACTTCGCCTTTGCCGCCAAGGACCTGGGACGT
TTCCGGGTCAGTGCTTTCTGGCAGCGTGAGTCTCCCGGTTGCGTAATGCGTCGTATCGAGACCAAAATTCCTGAGATGGA
AGACTTGAAACTGCCACCGGTGCTTAAAGATCTGGTGATGAGTAAACGCGGTCTTATCATTATGGTGGGTGGTACAGGTA
CGGGTAAATCGACGTCGCTGGCCGCCATGGTTGGGTATCGCAATGGCCATGAACGTGGTCATATCCTGACCATCGAAGAC
CCTGTGGAATTTGTGCACGATCACCGCAAGAGCATCATCACCCAGCGGGAAGTGGGGATCGATACCGAGTCCTTCGATGC
GGCGCTGAAAAGCTCGCTGCGTCAGGCCCCGGACGTGATCCTTATCGGTGAGATCCGCAGCCAGGAAACCATGGAATTTG
CGCTGTCATTTGCTGAGACAGGTCACCTGTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATGCACCTGGTGCCCGAAAGTAAGCACCAGCAGCTGTTGTTTGACCTGTCACTCAACCTGCGCGGTATTGTGGCACAGCA
ATTGGTACCCAAAGCCGATGGCACGGGGCGTCGTGCGGCCATTGAAATTCTGATTAACACCCCCCGTGTGTCGAGCATTA
TTCAGAAAAACGAGCTGCATTTGCTTAAAGACACCATGGCCAAGTCCAATGAGCAGGGGATGCAGACCTTTGACCAGGCG
CTGTTCAAGCTTTATCAGGAAGGTGAAATCACCTACGCCGATGCCTTGCACCATGCTGACTCGCCGAACGATCTGCGTTT
GATGATCAAACTGGCGGGCTCCGAAAGTACAGGTTCAGGCTTCCTGGAAGGCGTGACCCTGGACATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1S8H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.022

100

0.614
  pilU Vibrio cholerae strain A1552

59.189

100

0.592
  pilU Acinetobacter baylyi ADP1

60.057

95.405

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

97.297

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Acinetobacter nosocomialis M2

40.058

92.432

0.37
  pilT Acinetobacter baumannii D1279779

40.058

92.432

0.37
  pilT Acinetobacter baumannii strain A118

40.058

92.432

0.37
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.13

93.243

0.365
  pilT Pseudomonas aeruginosa PAK

39.13

93.243

0.365

Multiple sequence alignment