Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C2869_RS14030 Genome accession   NZ_CP026604
Coordinates   3894965..3896008 (+) Length   347 a.a.
NCBI ID   WP_108603535.1    Uniprot ID   A0A2S0VTE0
Organism   Saccharobesus litoralis strain CCB-QB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3889965..3901008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2869_RS14005 (C2869_14005) fdx 3891328..3891666 (+) 339 WP_108603531.1 ISC system 2Fe-2S type ferredoxin -
  C2869_RS14010 (C2869_14010) - 3891979..3892341 (-) 363 WP_228710670.1 DUF4426 domain-containing protein -
  C2869_RS14015 (C2869_14015) - 3892686..3893228 (-) 543 WP_108603532.1 YggT family protein -
  C2869_RS14020 (C2869_14020) proC 3893291..3894127 (-) 837 WP_108603533.1 pyrroline-5-carboxylate reductase -
  C2869_RS14025 (C2869_14025) - 3894147..3894854 (-) 708 WP_108603534.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2869_RS14030 (C2869_14030) pilT 3894965..3896008 (+) 1044 WP_108603535.1 type IV pilus twitching motility protein PilT Machinery gene
  C2869_RS14035 (C2869_14035) pilU 3896011..3897135 (+) 1125 WP_108603536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C2869_RS14040 (C2869_14040) def 3897285..3897836 (+) 552 WP_108603537.1 peptide deformylase -
  C2869_RS14045 (C2869_14045) asd 3897929..3899041 (+) 1113 WP_108603538.1 aspartate-semialdehyde dehydrogenase -
  C2869_RS22500 - 3899151..3899288 (+) 138 WP_159084178.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38469.15 Da        Isoelectric Point: 6.8923

>NTDB_id=270609 C2869_RS14030 WP_108603535.1 3894965..3896008(+) (pilT) [Saccharobesus litoralis strain CCB-QB4]
MDITELLAFSVEHKASDLHLSAGVSPMIRVDGEVRKINIPALDHKQVNQLIYDIMNDKQRKDYEENLESDFSFEVPNLAR
FRVNAFVQNRGAGAVFRTIPSEVLSLETLNAPDIFRDLADKPRGLVLVTGPTGSGKSTTLAAMINYINESKHHHILTIED
PIEFVHQNKLSLINQREVHRDTKSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTVDRI
VDVFPAAEKPMVRSMLSESLQAVISQTLLKKSGGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGIQQGMQTMDQCL
MNLVNSGQVSRQAAMQKAVNKQMFGGM

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=270609 C2869_RS14030 WP_108603535.1 3894965..3896008(+) (pilT) [Saccharobesus litoralis strain CCB-QB4]
ATGGATATTACAGAATTACTGGCCTTTAGCGTAGAACATAAAGCATCTGATTTACATTTATCAGCGGGTGTATCACCCAT
GATCCGTGTTGATGGTGAAGTACGCAAAATTAACATACCTGCCTTAGATCATAAACAAGTGAATCAGCTTATTTATGACA
TTATGAATGATAAGCAGCGCAAAGATTACGAAGAAAACTTAGAATCCGATTTCTCGTTTGAAGTTCCTAATCTTGCTCGA
TTCAGGGTTAATGCTTTTGTACAAAACCGCGGCGCTGGCGCTGTGTTTCGTACCATCCCAAGTGAAGTTTTATCTTTAGA
AACCCTTAACGCGCCCGACATTTTCCGTGATTTGGCCGACAAACCTCGCGGTTTGGTATTAGTTACAGGGCCAACGGGTT
CGGGTAAGTCAACCACTTTGGCGGCCATGATTAACTATATCAATGAGTCTAAGCATCACCATATTTTAACCATTGAGGAC
CCAATTGAATTTGTTCATCAAAATAAATTGAGTTTGATTAACCAAAGGGAAGTGCATCGCGATACTAAGTCGTTTAATAA
CGCATTACGTTCTGCTTTACGTGAAGATCCTGATGTTATCCTAGTGGGTGAGATGCGTGACCTTGAAACTATCCGCTTAG
CTATGACAGCTGCCGAAACCGGTCACTTAGTTTTTGGAACCCTGCATACTACGTCAGCGCCGAAAACGGTTGACCGTATC
GTTGACGTGTTCCCTGCAGCAGAAAAACCTATGGTCCGTTCAATGCTATCGGAATCCTTGCAAGCGGTTATTTCCCAAAC
CTTATTGAAAAAGTCGGGTGGTGGCCGAGTTGCCGCTCACGAAATCATGGTGGGTACTCCGGCTATTCGTAACTTGATCC
GTGAAGATAAAGTCGCTCAAATGTATTCTGCTATTCAAACGGGTATACAGCAAGGTATGCAGACCATGGATCAATGTTTA
ATGAACTTGGTTAACTCTGGTCAAGTATCACGCCAGGCCGCGATGCAAAAAGCGGTTAATAAACAAATGTTTGGAGGCAT
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0VTE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.135

0.767
  pilT Acinetobacter nosocomialis M2

77.326

99.135

0.767
  pilT Acinetobacter baumannii D1279779

77.326

99.135

0.767
  pilT Acinetobacter baumannii strain A118

77.326

99.135

0.767
  pilT Pseudomonas aeruginosa PAK

78.107

97.406

0.761
  pilT Acinetobacter baylyi ADP1

76.744

99.135

0.761
  pilT Legionella pneumophila strain Lp02

73.256

99.135

0.726
  pilT Legionella pneumophila strain ERS1305867

73.256

99.135

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.118

0.692
  pilT Vibrio cholerae strain A1552

71.217

97.118

0.692
  pilT Neisseria gonorrhoeae MS11

69.388

98.847

0.686
  pilT Neisseria meningitidis 8013

69.388

98.847

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.593

97.695

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.298

97.695

0.403
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401

Multiple sequence alignment