Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C2869_RS14035 Genome accession   NZ_CP026604
Coordinates   3896011..3897135 (+) Length   374 a.a.
NCBI ID   WP_108603536.1    Uniprot ID   A0A2S0VTF2
Organism   Saccharobesus litoralis strain CCB-QB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3891011..3902135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2869_RS14005 (C2869_14005) fdx 3891328..3891666 (+) 339 WP_108603531.1 ISC system 2Fe-2S type ferredoxin -
  C2869_RS14010 (C2869_14010) - 3891979..3892341 (-) 363 WP_228710670.1 DUF4426 domain-containing protein -
  C2869_RS14015 (C2869_14015) - 3892686..3893228 (-) 543 WP_108603532.1 YggT family protein -
  C2869_RS14020 (C2869_14020) proC 3893291..3894127 (-) 837 WP_108603533.1 pyrroline-5-carboxylate reductase -
  C2869_RS14025 (C2869_14025) - 3894147..3894854 (-) 708 WP_108603534.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2869_RS14030 (C2869_14030) pilT 3894965..3896008 (+) 1044 WP_108603535.1 type IV pilus twitching motility protein PilT Machinery gene
  C2869_RS14035 (C2869_14035) pilU 3896011..3897135 (+) 1125 WP_108603536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C2869_RS14040 (C2869_14040) def 3897285..3897836 (+) 552 WP_108603537.1 peptide deformylase -
  C2869_RS14045 (C2869_14045) asd 3897929..3899041 (+) 1113 WP_108603538.1 aspartate-semialdehyde dehydrogenase -
  C2869_RS22500 - 3899151..3899288 (+) 138 WP_159084178.1 hypothetical protein -
  C2869_RS14050 (C2869_14050) - 3899575..3901065 (+) 1491 WP_108603539.1 AraC family transcriptional regulator -
  C2869_RS14055 (C2869_14055) - 3901666..3901884 (+) 219 WP_108603540.1 RebB family R body protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41389.57 Da        Isoelectric Point: 5.6678

>NTDB_id=270610 C2869_RS14035 WP_108603536.1 3896011..3897135(+) (pilU) [Saccharobesus litoralis strain CCB-QB4]
MLLAPYLKDMCDKNGSDLFIAVGRPVCAKINGEMTPLDEEVVVEEHARELALDSMTDKQKDEFLTTSECNYAVAIDGVGR
FRCSAFVQREKVGMVIRRINTEIPDADKLGLPNVLKEVIMSKRGLFLYVGATGTGKSTALAALMGYRNRNSKGHILTIED
PIEFVHKHEKCIVNQREVGTDTESFDAALKSSLRQAPDVIMIGEIRSKEIMEYALSFAETGHLCVATLHANNANQAIDRI
MHLVPKDMKEKLLFDLSLNLRAICAQQLVPTKDGNGRVAAIEIMLNSPLISDMIARGDIGAIKEVMDKSKEQGMQTFDGA
LFELYNQGKINFSDALHHADSPNDLRLKIKLRTNETRSSGFLDGVTVDFDDGPK

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=270610 C2869_RS14035 WP_108603536.1 3896011..3897135(+) (pilU) [Saccharobesus litoralis strain CCB-QB4]
ATGTTACTCGCTCCTTATTTAAAAGACATGTGCGATAAAAATGGCTCTGACTTGTTTATCGCTGTTGGCCGTCCAGTTTG
CGCCAAAATTAATGGCGAAATGACCCCGTTAGATGAAGAAGTTGTCGTAGAAGAGCATGCTCGAGAGCTAGCACTAGACT
CGATGACGGATAAACAAAAGGATGAGTTTTTAACCACTAGTGAGTGTAACTACGCGGTGGCTATTGACGGTGTTGGACGT
TTTCGTTGTAGTGCGTTTGTCCAGCGCGAAAAAGTGGGTATGGTTATTCGCCGTATTAATACCGAGATCCCAGATGCGGA
CAAATTAGGTTTACCTAACGTATTAAAAGAAGTGATCATGTCTAAGCGTGGTTTATTTTTGTACGTGGGTGCGACAGGTA
CTGGTAAATCAACCGCACTCGCGGCATTAATGGGCTATCGTAATCGTAATTCAAAAGGCCATATTTTAACCATTGAAGAC
CCGATTGAATTTGTGCACAAGCATGAAAAATGCATTGTTAACCAACGTGAAGTGGGGACAGACACAGAATCATTCGATGC
CGCGCTGAAAAGCTCGTTACGTCAGGCGCCAGACGTCATTATGATTGGTGAAATTCGCTCTAAAGAAATTATGGAGTATG
CGTTAAGCTTTGCGGAAACAGGGCATTTGTGTGTTGCGACACTGCATGCTAATAACGCCAACCAAGCTATCGATCGTATC
ATGCACTTAGTACCAAAAGATATGAAAGAAAAGCTGTTGTTTGATTTGTCACTGAATTTACGCGCCATCTGTGCTCAGCA
ATTAGTGCCAACAAAAGACGGTAACGGTCGGGTTGCGGCAATCGAAATTATGCTCAATTCTCCACTTATCTCAGATATGA
TCGCCCGTGGTGATATTGGCGCAATTAAAGAGGTGATGGACAAATCAAAAGAGCAGGGGATGCAAACGTTTGATGGCGCC
TTATTTGAACTTTACAATCAAGGTAAAATTAACTTTTCAGATGCATTACATCACGCCGATTCGCCGAATGATTTACGCCT
TAAAATTAAATTGCGCACTAACGAAACTCGAAGTTCAGGTTTCTTAGATGGTGTTACGGTTGATTTTGATGACGGCCCTA
AATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0VTF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.676

98.93

0.551
  pilU Vibrio cholerae strain A1552

55.135

98.93

0.545
  pilU Acinetobacter baylyi ADP1

57.143

91.711

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

90.642

0.369

Multiple sequence alignment