Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A5904_RS12150 Genome accession   NZ_CP026328
Coordinates   2447946..2448995 (-) Length   349 a.a.
NCBI ID   WP_004869054.1    Uniprot ID   A0A1E7YRY7
Organism   Acidithiobacillus caldus strain MTH-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442946..2453995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5904_RS12125 (A5904_12545) - 2443098..2443805 (+) 708 WP_014003505.1 MBL fold metallo-hydrolase -
  A5904_RS12130 (A5904_12550) - 2443798..2444307 (+) 510 WP_014003506.1 hypothetical protein -
  A5904_RS12135 (A5904_12555) - 2444441..2445574 (+) 1134 WP_004869047.1 NAD(P)/FAD-dependent oxidoreductase -
  A5904_RS12140 (A5904_12560) - 2445644..2446825 (-) 1182 WP_064306315.1 glycosyltransferase -
  A5904_RS12145 (A5904_12565) pilU 2446807..2447937 (-) 1131 WP_014003509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A5904_RS12150 (A5904_12570) pilT 2447946..2448995 (-) 1050 WP_004869054.1 type IV pilus twitching motility protein PilT Machinery gene
  A5904_RS12155 (A5904_12575) - 2449066..2449758 (+) 693 WP_226047714.1 YggS family pyridoxal phosphate-dependent enzyme -
  A5904_RS12160 (A5904_12580) proC 2449755..2450585 (+) 831 WP_014003511.1 pyrroline-5-carboxylate reductase -
  A5904_RS12165 (A5904_12585) - 2450582..2450863 (+) 282 WP_014003512.1 YggT family protein -
  A5904_RS12170 (A5904_12590) - 2450883..2451230 (+) 348 WP_004873263.1 DUF167 domain-containing protein -
  A5904_RS12175 (A5904_12595) bioB 2451202..2452149 (+) 948 WP_004873262.1 biotin synthase BioB -
  A5904_RS12180 (A5904_12600) - 2452194..2452748 (-) 555 WP_004873261.1 acyloxyacyl hydrolase -
  A5904_RS12185 (A5904_12605) - 2452925..2453938 (+) 1014 WP_014003513.1 HlyD family secretion protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38578.33 Da        Isoelectric Point: 6.9775

>NTDB_id=268254 A5904_RS12150 WP_004869054.1 2447946..2448995(-) (pilT) [Acidithiobacillus caldus strain MTH-04]
MDITELLTFAVKNNASDTHMSAGLPPMLRIHGDIRHLNVEPLDAKTVHAMIYDIMNDAQRKWFEENLEIDFAIDLAGVAR
FRVNAFNQDRGPAAAFRTIPAKVLSLEDLNAPKVFKDIINVPRGLVLVTGPTGSGKSTTLAAMVDHINQQRADHIITIED
PIEFLHTPKKCLINQREVGPHTHSFANALRSALREDPDVILVGELRDLETMRLALTAAETGHVVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVAAHEIMIANPAIRNLIRENKIAQMYSVIQTGQNQGMQTLDQC
LADLVRAHRITREEALRRVQNKDSFAAVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=268254 A5904_RS12150 WP_004869054.1 2447946..2448995(-) (pilT) [Acidithiobacillus caldus strain MTH-04]
GTGGATATCACCGAACTGCTGACTTTTGCCGTCAAGAACAATGCCTCCGACACCCACATGTCCGCCGGCTTGCCGCCCAT
GTTGCGCATCCATGGCGACATCCGCCATCTCAATGTCGAGCCCCTGGACGCCAAGACGGTACACGCCATGATCTACGACA
TCATGAACGATGCCCAGCGCAAATGGTTTGAAGAAAATCTCGAGATCGACTTCGCCATCGATCTGGCCGGGGTGGCGCGC
TTCCGTGTCAATGCCTTCAATCAGGACCGTGGGCCGGCGGCGGCCTTCCGCACCATTCCCGCCAAGGTGCTGAGCCTCGA
GGATCTGAATGCGCCCAAGGTCTTCAAGGATATCATCAACGTTCCACGCGGTCTGGTCCTGGTGACGGGGCCCACGGGAT
CGGGCAAATCCACGACCCTGGCCGCCATGGTCGATCACATCAACCAGCAGCGTGCCGACCACATCATCACCATCGAGGAT
CCCATCGAATTCCTCCACACCCCCAAAAAATGCCTCATCAACCAGCGCGAGGTGGGGCCACATACCCACTCCTTTGCCAA
TGCCCTGCGCTCGGCCCTGCGTGAGGATCCGGACGTCATTCTCGTGGGCGAGTTGCGCGATCTGGAGACCATGCGCCTGG
CCCTTACCGCTGCGGAAACCGGCCACGTCGTCTTCGCGACCCTGCACACGAGCTCGGCGCCGAAAACCATCGATCGTATC
GTCGACTCCTTCCCCGGCGGCGAGAAGGACATGGTGCGGGCCATGCTGTCGGAGTCCCTGCGCGCGGTCATCTCCCAGAC
CCTCCTCAAAACGGCGGATGGCAAGGGCCGAGTGGCCGCCCACGAAATCATGATTGCCAACCCGGCCATCCGCAACCTCA
TCCGCGAAAACAAGATTGCGCAGATGTACTCGGTGATTCAAACCGGTCAGAATCAGGGCATGCAGACCCTGGATCAGTGT
CTTGCGGACCTCGTCCGCGCCCACCGTATCACCCGCGAGGAGGCGCTTCGGCGGGTGCAGAACAAAGACAGCTTCGCTGC
CGTCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E7YRY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

68.406

98.854

0.676
  pilT Acinetobacter baumannii D1279779

68.406

98.854

0.676
  pilT Acinetobacter baumannii strain A118

68.406

98.854

0.676
  pilT Pseudomonas aeruginosa PAK

68.116

98.854

0.673
  pilT Pseudomonas stutzeri DSM 10701

67.826

98.854

0.67
  pilT Neisseria meningitidis 8013

66.763

99.14

0.662
  pilT Acinetobacter baylyi ADP1

66.667

98.854

0.659
  pilT Neisseria gonorrhoeae MS11

66.474

99.14

0.659
  pilT Legionella pneumophila strain Lp02

65.507

98.854

0.648
  pilT Legionella pneumophila strain ERS1305867

65.507

98.854

0.648
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65

97.421

0.633
  pilT Vibrio cholerae strain A1552

65

97.421

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.009

100

0.53
  pilU Acinetobacter baylyi ADP1

42.899

96.848

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.262

96.275

0.407
  pilU Vibrio cholerae strain A1552

40.476

96.275

0.39

Multiple sequence alignment