Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A5904_RS12145 Genome accession   NZ_CP026328
Coordinates   2446807..2447937 (-) Length   376 a.a.
NCBI ID   WP_014003509.1    Uniprot ID   A0A1E7YS40
Organism   Acidithiobacillus caldus strain MTH-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2441807..2452937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5904_RS12120 (A5904_12540) - 2442268..2442963 (+) 696 WP_233444821.1 Crp/Fnr family transcriptional regulator -
  A5904_RS12125 (A5904_12545) - 2443098..2443805 (+) 708 WP_014003505.1 MBL fold metallo-hydrolase -
  A5904_RS12130 (A5904_12550) - 2443798..2444307 (+) 510 WP_014003506.1 hypothetical protein -
  A5904_RS12135 (A5904_12555) - 2444441..2445574 (+) 1134 WP_004869047.1 NAD(P)/FAD-dependent oxidoreductase -
  A5904_RS12140 (A5904_12560) - 2445644..2446825 (-) 1182 WP_064306315.1 glycosyltransferase -
  A5904_RS12145 (A5904_12565) pilU 2446807..2447937 (-) 1131 WP_014003509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A5904_RS12150 (A5904_12570) pilT 2447946..2448995 (-) 1050 WP_004869054.1 type IV pilus twitching motility protein PilT Machinery gene
  A5904_RS12155 (A5904_12575) - 2449066..2449758 (+) 693 WP_226047714.1 YggS family pyridoxal phosphate-dependent enzyme -
  A5904_RS12160 (A5904_12580) proC 2449755..2450585 (+) 831 WP_014003511.1 pyrroline-5-carboxylate reductase -
  A5904_RS12165 (A5904_12585) - 2450582..2450863 (+) 282 WP_014003512.1 YggT family protein -
  A5904_RS12170 (A5904_12590) - 2450883..2451230 (+) 348 WP_004873263.1 DUF167 domain-containing protein -
  A5904_RS12175 (A5904_12595) bioB 2451202..2452149 (+) 948 WP_004873262.1 biotin synthase BioB -
  A5904_RS12180 (A5904_12600) - 2452194..2452748 (-) 555 WP_004873261.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41805.15 Da        Isoelectric Point: 6.0023

>NTDB_id=268253 A5904_RS12145 WP_014003509.1 2446807..2447937(-) (pilU) [Acidithiobacillus caldus strain MTH-04]
MAALEQLLQLMVQKNASDLYLTVGAPPTLKIDGRAVPIGQEALRPGQTLALAKEILGLERLQEFQQEKEVNMAISASGIG
RFRVNGFFQRGELGFVLRAIKTDIPTLEQLKMPDVLKSLAMSARGLVLFVGATGSGKSTSLASMIQYRNQNSPGHILTIE
DPIEFLHKNYQSIVNQREVGIDTLSYENALENALREAPDVILIGEIRSRDTMDHAIAYAETGHLCLSTLHANNANQAIER
IINFFPEDRKRQVLMDLSLNLRAVISQRLLPLKDRPGRMAAMEILINTPAIADLIYKGEVGLLKDAMARTNDVGMQTFDQ
SLLKLYMDGLIHYEDALRGADSQNDLRLAIKMECLRRGLEDPGAQSDGERQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=268253 A5904_RS12145 WP_014003509.1 2446807..2447937(-) (pilU) [Acidithiobacillus caldus strain MTH-04]
ATGGCCGCCCTCGAACAGTTGCTGCAATTGATGGTGCAGAAGAACGCCTCGGACCTGTATCTCACCGTCGGTGCTCCACC
CACCCTGAAGATCGACGGTCGCGCGGTTCCCATCGGTCAGGAGGCCCTGCGGCCCGGCCAAACCTTGGCCCTGGCCAAGG
AGATCCTGGGTCTGGAGCGTCTGCAGGAGTTCCAGCAGGAAAAGGAGGTCAACATGGCGATCTCGGCCAGTGGTATCGGC
CGTTTTCGCGTCAATGGCTTCTTCCAGCGTGGCGAGCTGGGTTTCGTGCTGCGCGCCATCAAGACCGACATCCCCACCCT
TGAGCAGCTGAAGATGCCCGACGTGCTCAAGAGTCTCGCTATGTCGGCGCGGGGACTGGTGCTTTTCGTGGGGGCAACCG
GTTCGGGCAAGAGTACCTCCCTTGCGTCCATGATCCAGTATCGTAACCAGAACTCCCCCGGGCACATCCTCACCATCGAG
GACCCCATCGAGTTCCTGCATAAGAATTACCAATCCATCGTCAATCAGCGGGAAGTGGGCATCGACACCCTGAGCTACGA
AAATGCCCTGGAAAATGCCCTGCGCGAGGCCCCGGACGTCATCCTCATCGGCGAAATCCGCAGCCGCGACACCATGGACC
ACGCCATTGCCTATGCTGAGACGGGCCACCTCTGTCTGTCGACCCTGCACGCCAACAACGCCAACCAGGCCATCGAGCGC
ATCATCAATTTCTTCCCCGAGGACCGCAAGCGCCAGGTGCTGATGGATCTCTCCCTCAACCTGCGCGCCGTGATATCCCA
GCGACTCCTGCCCCTCAAGGATCGGCCGGGACGGATGGCGGCCATGGAGATTCTGATCAATACCCCAGCCATCGCCGATC
TGATCTACAAGGGCGAGGTAGGCCTGCTCAAGGACGCCATGGCACGCACCAACGATGTCGGCATGCAGACCTTTGACCAG
TCCCTCCTGAAGCTCTACATGGACGGTCTGATCCATTATGAGGACGCTCTGCGCGGGGCGGACTCGCAGAATGACCTGCG
CCTTGCCATCAAGATGGAATGCCTGCGTCGTGGCCTCGAGGATCCCGGCGCGCAGAGCGATGGCGAACGGCAGTGGCGTA
TCCAGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E7YS40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

97.606

0.585
  pilU Vibrio cholerae strain A1552

55.372

96.543

0.535
  pilU Acinetobacter baylyi ADP1

54.348

97.872

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.516

92.287

0.402
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.512

87.234

0.388
  pilT Vibrio cholerae strain A1552

44.512

87.234

0.388
  pilT Pseudomonas aeruginosa PAK

43.243

88.564

0.383
  pilT Pseudomonas stutzeri DSM 10701

42.943

88.564

0.38
  pilT Neisseria meningitidis 8013

41.691

91.223

0.38
  pilT Neisseria gonorrhoeae MS11

41.228

90.957

0.375
  pilT Acinetobacter baylyi ADP1

42.042

88.564

0.372
  pilT Acinetobacter baumannii D1279779

41.246

89.628

0.37
  pilT Acinetobacter baumannii strain A118

41.246

89.628

0.37
  pilT Acinetobacter nosocomialis M2

41.246

89.628

0.37
  pilT Legionella pneumophila strain Lp02

40.356

89.628

0.362
  pilT Legionella pneumophila strain ERS1305867

40.356

89.628

0.362

Multiple sequence alignment