Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C2U30_RS17515 Genome accession   NZ_CP026122
Coordinates   3722003..3723037 (-) Length   344 a.a.
NCBI ID   WP_103244191.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH5     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3717003..3728037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U30_RS17495 (C2U30_17500) - 3717818..3718528 (-) 711 WP_043153021.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  C2U30_RS17500 (C2U30_17505) srmB 3718656..3719876 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  C2U30_RS17505 (C2U30_17510) yaaA 3720064..3720837 (-) 774 WP_103244190.1 peroxide stress protein YaaA -
  C2U30_RS17510 (C2U30_17515) pilU 3720870..3721979 (-) 1110 WP_041214461.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C2U30_RS17515 (C2U30_17520) pilT 3722003..3723037 (-) 1035 WP_103244191.1 type IVa pilus ATPase TapT Machinery gene
  C2U30_RS17520 (C2U30_17525) - 3723077..3723778 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U30_RS17525 (C2U30_17530) proC 3723901..3724725 (+) 825 WP_041210938.1 pyrroline-5-carboxylate reductase -
  C2U30_RS17530 (C2U30_17535) - 3724749..3725300 (+) 552 WP_010673053.1 YggT family protein -
  C2U30_RS17535 (C2U30_17540) yggU 3725300..3725599 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  C2U30_RS17540 (C2U30_17545) - 3725618..3726037 (+) 420 WP_029313762.1 DUF4426 domain-containing protein -
  C2U30_RS17545 (C2U30_17550) - 3726188..3727390 (+) 1203 WP_076611394.1 ISL3-like element ISAeme19 family transposase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38146.87 Da        Isoelectric Point: 7.0205

>NTDB_id=267335 C2U30_RS17515 WP_103244191.1 3722003..3723037(-) (pilT) [Aeromonas sp. ASNIH5]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSKSLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGGVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=267335 C2U30_RS17515 WP_103244191.1 3722003..3723037(-) (pilT) [Aeromonas sp. ASNIH5]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCATCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGACCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGCCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCAAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGGCGTCGCCTCCCTCGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Pseudomonas stutzeri DSM 10701

78.006

99.128

0.773
  pilT Acinetobacter baumannii D1279779

76.453

100

0.765
  pilT Acinetobacter baumannii strain A118

76.453

100

0.765
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Pseudomonas aeruginosa PAK

76.54

99.128

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

71.176

98.837

0.703
  pilT Legionella pneumophila strain ERS1305867

71.176

98.837

0.703
  pilT Neisseria meningitidis 8013

68.732

98.547

0.677
  pilT Neisseria gonorrhoeae MS11

68.437

98.547

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment