Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C2U30_RS17510 Genome accession   NZ_CP026122
Coordinates   3720870..3721979 (-) Length   369 a.a.
NCBI ID   WP_041214461.1    Uniprot ID   A0A839D3X0
Organism   Aeromonas sp. ASNIH5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3715870..3726979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U30_RS17485 (C2U30_17490) - 3716003..3716230 (+) 228 WP_103244189.1 hypothetical protein -
  C2U30_RS17490 (C2U30_17495) brnQ 3716320..3717582 (-) 1263 WP_233720955.1 branched-chain amino acid transport system II carrier protein -
  C2U30_RS17495 (C2U30_17500) - 3717818..3718528 (-) 711 WP_043153021.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  C2U30_RS17500 (C2U30_17505) srmB 3718656..3719876 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  C2U30_RS17505 (C2U30_17510) yaaA 3720064..3720837 (-) 774 WP_103244190.1 peroxide stress protein YaaA -
  C2U30_RS17510 (C2U30_17515) pilU 3720870..3721979 (-) 1110 WP_041214461.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C2U30_RS17515 (C2U30_17520) pilT 3722003..3723037 (-) 1035 WP_103244191.1 type IVa pilus ATPase TapT Machinery gene
  C2U30_RS17520 (C2U30_17525) - 3723077..3723778 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U30_RS17525 (C2U30_17530) proC 3723901..3724725 (+) 825 WP_041210938.1 pyrroline-5-carboxylate reductase -
  C2U30_RS17530 (C2U30_17535) - 3724749..3725300 (+) 552 WP_010673053.1 YggT family protein -
  C2U30_RS17535 (C2U30_17540) yggU 3725300..3725599 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  C2U30_RS17540 (C2U30_17545) - 3725618..3726037 (+) 420 WP_029313762.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40856.99 Da        Isoelectric Point: 6.3155

>NTDB_id=267334 C2U30_RS17510 WP_041214461.1 3720870..3721979(-) (pilU) [Aeromonas sp. ASNIH5]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=267334 C2U30_RS17510 WP_041214461.1 3720870..3721979(-) (pilU) [Aeromonas sp. ASNIH5]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCAGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTTTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACATTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839D3X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

91.057

0.393
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment