Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MC63_RS05760 Genome accession   NZ_CP026098
Coordinates   1163812..1164792 (-) Length   326 a.a.
NCBI ID   WP_094099480.1    Uniprot ID   -
Organism   Shigella flexneri strain FDAARGOS_74     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1158812..1169792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MC63_RS05730 (MC63_006055) - 1159475..1159972 (+) 498 WP_005051770.1 SprT family zinc-dependent metalloprotease -
  MC63_RS05735 (MC63_006060) endA 1160067..1160774 (+) 708 WP_040235105.1 deoxyribonuclease I -
  MC63_RS05740 (MC63_006065) rsmE 1160854..1161585 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MC63_RS05745 (MC63_006070) gshB 1161598..1162548 (+) 951 WP_000593273.1 glutathione synthase -
  MC63_RS05750 (MC63_006075) - 1162657..1163220 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  MC63_RS05755 (MC63_006080) ruvX 1163220..1163636 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  MC63_RS05760 (MC63_006085) pilT 1163812..1164792 (-) 981 WP_094099480.1 type IV pilus twitching motility protein PilT Machinery gene
  MC63_RS05765 (MC63_006090) yggS 1164810..1165514 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  MC63_RS05770 (MC63_006095) yggT 1165532..1166098 (+) 567 WP_001094819.1 osmotic shock tolerance protein YggT -
  MC63_RS05775 (MC63_006100) yggU 1166095..1166385 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  MC63_RS05780 (MC63_006105) - 1166393..1166986 (+) 594 WP_025759617.1 XTP/dITP diphosphatase -
  MC63_RS05785 (MC63_006110) hemW 1166979..1168115 (+) 1137 WP_025759619.1 radical SAM family heme chaperone HemW -
  MC63_RS05790 (MC63_006115) - 1168429..1169392 (+) 964 Protein_1110 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35898.11 Da        Isoelectric Point: 5.9959

>NTDB_id=267136 MC63_RS05760 WP_094099480.1 1163812..1164792(-) (pilT) [Shigella flexneri strain FDAARGOS_74]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKAPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=267136 MC63_RS05760 WP_094099480.1 1163812..1164792(-) (pilT) [Shigella flexneri strain FDAARGOS_74]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGCTCCCGTACGTAATCAACTTGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment