Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1S74_RS13445 Genome accession   NZ_CP025794
Coordinates   2712148..2713188 (+) Length   346 a.a.
NCBI ID   WP_038873181.1    Uniprot ID   A0A2N7N1P7
Organism   Vibrio hyugaensis strain 090810a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707148..2718188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S74_RS13410 - 2708089..2708691 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  C1S74_RS13420 - 2709063..2709494 (-) 432 WP_045397705.1 DUF4426 domain-containing protein -
  C1S74_RS13425 yggU 2709558..2709848 (-) 291 WP_038873189.1 DUF167 family protein YggU -
  C1S74_RS13430 - 2709848..2710405 (-) 558 WP_038873187.1 YggT family protein -
  C1S74_RS13435 proC 2710460..2711278 (-) 819 WP_045397706.1 pyrroline-5-carboxylate reductase -
  C1S74_RS13440 - 2711409..2712119 (-) 711 WP_045397709.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1S74_RS13445 pilT 2712148..2713188 (+) 1041 WP_038873181.1 type IV pilus twitching motility protein PilT Machinery gene
  C1S74_RS13450 pilU 2713219..2714325 (+) 1107 WP_038873178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1S74_RS13455 ruvX 2714386..2714811 (-) 426 WP_038868128.1 Holliday junction resolvase RuvX -
  C1S74_RS13460 - 2714865..2715428 (-) 564 WP_038868129.1 YqgE/AlgH family protein -
  C1S74_RS13465 gshB 2715540..2716490 (-) 951 WP_045397711.1 glutathione synthase -
  C1S74_RS13470 rsmE 2716505..2717236 (-) 732 WP_038868134.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1S74_RS13475 endA 2717384..2718079 (-) 696 WP_038881140.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38633.13 Da        Isoelectric Point: 6.5043

>NTDB_id=263845 C1S74_RS13445 WP_038873181.1 2712148..2713188(+) (pilT) [Vibrio hyugaensis strain 090810a]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=263845 C1S74_RS13445 WP_038873181.1 2712148..2713188(+) (pilT) [Vibrio hyugaensis strain 090810a]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAATTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCACAATGTTGGCCGT
TTTCGTGTCAACGCCTTCAACCAATCTCGTGGCTGTGCGGCGGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAGATTGCTAACGCAGAAAAAGGTTTAGTACTGGTTACCGGTCCAACAGGTT
CGGGTAAATCGACAACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTTACAATTGAAGAT
CCTATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAAGTACACCGTGATACGCATAGCTTCCAAAA
TGCGCTTCGCAGCGCGCTACGTGAAGACCCGGATGTCATTCTAGTTGGTGAGATGCGTGACAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCATACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCAGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCGGAGTCGTTGCGTTCGGTTATTGCGCAAAA
GCTGCTTAAGCGAAATGGTGGAGGCCGTATTGCTTGTCATGAGATCATGATGGCAACGCCAGCAATCCGTAACTTGATCC
GCGAAGATAAAGTGGCGCAGATGTACTCGATCATTCAAACCGGTGCGGCACATGGCATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCTCAAGGTATGGTATCGCGTGAAGAAGTCGACAAGAAAGTCGAAATCGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7N1P7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.727

95.376

0.694
  pilT Acinetobacter baumannii strain A118

72.727

95.376

0.694
  pilT Acinetobacter nosocomialis M2

72.424

95.376

0.691
  pilT Pseudomonas aeruginosa PAK

69.412

98.266

0.682
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

68.278

95.665

0.653
  pilT Legionella pneumophila strain ERS1305867

68.278

95.665

0.653
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364

Multiple sequence alignment