Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C1S74_RS13450 Genome accession   NZ_CP025794
Coordinates   2713219..2714325 (+) Length   368 a.a.
NCBI ID   WP_038873178.1    Uniprot ID   A0A2N7N1P0
Organism   Vibrio hyugaensis strain 090810a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708219..2719325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S74_RS13420 - 2709063..2709494 (-) 432 WP_045397705.1 DUF4426 domain-containing protein -
  C1S74_RS13425 yggU 2709558..2709848 (-) 291 WP_038873189.1 DUF167 family protein YggU -
  C1S74_RS13430 - 2709848..2710405 (-) 558 WP_038873187.1 YggT family protein -
  C1S74_RS13435 proC 2710460..2711278 (-) 819 WP_045397706.1 pyrroline-5-carboxylate reductase -
  C1S74_RS13440 - 2711409..2712119 (-) 711 WP_045397709.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1S74_RS13445 pilT 2712148..2713188 (+) 1041 WP_038873181.1 type IV pilus twitching motility protein PilT Machinery gene
  C1S74_RS13450 pilU 2713219..2714325 (+) 1107 WP_038873178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1S74_RS13455 ruvX 2714386..2714811 (-) 426 WP_038868128.1 Holliday junction resolvase RuvX -
  C1S74_RS13460 - 2714865..2715428 (-) 564 WP_038868129.1 YqgE/AlgH family protein -
  C1S74_RS13465 gshB 2715540..2716490 (-) 951 WP_045397711.1 glutathione synthase -
  C1S74_RS13470 rsmE 2716505..2717236 (-) 732 WP_038868134.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C1S74_RS13475 endA 2717384..2718079 (-) 696 WP_038881140.1 deoxyribonuclease I -
  C1S74_RS13480 - 2718230..2718727 (-) 498 WP_045397713.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41312.46 Da        Isoelectric Point: 6.7730

>NTDB_id=263846 C1S74_RS13450 WP_038873178.1 2713219..2714325(+) (pilU) [Vibrio hyugaensis strain 090810a]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVAMLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKTTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=263846 C1S74_RS13450 WP_038873178.1 2713219..2714325(+) (pilU) [Vibrio hyugaensis strain 090810a]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTTTATATCACGGTTGGTGCGCCGATCTT
ATTTCGTGTGGATGGCGAACTGCGTCCGCAAGGGGATAAGTTGAGTGAAAATGACGTAGCCATGCTGCTCGATAGTGCAA
TGGATCCAGAGCGACGTCAGGAGTTTCGTAAAAGTCGAGAATCAAACTTTGCGATTGTCCGAGATTGTGGCCGCTTCCGT
GTTAGTGCTTTCTTTCAGCGAGAATTACCTGGTGCGGTGATTCGTCGTATCGAAACCAACATTCCTACGTTTGAACAGCT
TAAGTTGCCTTTAGTGTTGCAAGATCTTGCGATAGCGAAACGTGGCTTGGTGCTGGTGGTTGGTGCTACAGGTTCCGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTACCGTAACAGCAATAAAACAGGTCACATTCTGACAGTTGAAGACCCAATC
GAATTTGTGCACGAGCACAAGCGTTGTATCGTGACTCAGCGTGAAGTGGGATTGGATACCGACAGCTATGAAGTAGCGTT
AAAGAACTCATTACGCCAAGCGCCTGACATGATTTTGATTGGTGAGATCCGTAGCCGCGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCTACATTGCACGCGAACAACGCAAACCAAGCACTTGAGCGTATTTTGCAC
CTAGTGCCAAAAGATCAGAAAGATCAGTTCCTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAACAGCTGAT
CCGTGATAAAAACGGTCAAGGACGTCATGGAGTGTTTGAGATTCTGCTAAATAGTCCACGAGTTTCTGATCTGATCCGTC
GCGGTGATCTGCATGAGTTAAAAACCACCATGGCACGCTCTAATGAGTTCGGTATGCTGACGTTTGATCAGTCGCTCTAT
AAATTGGTGATGCAAGGCAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGATTCAGCTAACGATCTGCGTTTGATGTT
GAAGACGCAACGCGGTGAAGCCTTCTCTACAGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7N1P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.793

100

0.818
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.279

93.478

0.386
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment