Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C1H71_RS04960 Genome accession   NZ_CP025781
Coordinates   1142286..1143326 (-) Length   346 a.a.
NCBI ID   WP_130105582.1    Uniprot ID   A0A7G3G6S3
Organism   Iodobacter fluviatilis strain PCH194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1137286..1148326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1H71_RS04930 (C1H71_04980) - 1138090..1138695 (-) 606 WP_130105576.1 peroxiredoxin -
  C1H71_RS04935 - 1139040..1139222 (-) 183 WP_130105577.1 hypothetical protein -
  C1H71_RS04940 (C1H71_04985) - 1139410..1140084 (+) 675 WP_130105578.1 methyltransferase -
  C1H71_RS04950 (C1H71_04990) - 1140627..1140878 (-) 252 WP_188053595.1 FimV family protein -
  C1H71_RS04955 (C1H71_04995) pilU 1140886..1142022 (-) 1137 WP_130105581.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1H71_RS04960 (C1H71_05000) pilT 1142286..1143326 (-) 1041 WP_130105582.1 type IV pilus twitching motility protein PilT Machinery gene
  C1H71_RS04965 (C1H71_05005) - 1143505..1144218 (+) 714 WP_188053598.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1H71_RS04970 (C1H71_05010) proC 1144258..1145058 (+) 801 WP_130105584.1 pyrroline-5-carboxylate reductase -
  C1H71_RS04975 (C1H71_05015) - 1145058..1145651 (+) 594 WP_130105585.1 YggT family protein -
  C1H71_RS04980 (C1H71_05020) - 1145804..1146229 (-) 426 WP_130105586.1 FxsA family protein -
  C1H71_RS04985 (C1H71_05025) cutA 1146228..1146572 (+) 345 WP_130105587.1 divalent-cation tolerance protein CutA -
  C1H71_RS04990 (C1H71_05030) dsbD 1146569..1148104 (+) 1536 WP_130105588.1 protein-disulfide reductase DsbD -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38469.36 Da        Isoelectric Point: 6.7679

>NTDB_id=263737 C1H71_RS04960 WP_130105582.1 1142286..1143326(-) (pilT) [Iodobacter fluviatilis strain PCH194]
MEISELLAFTVKNKASDLHLSSGLPPMIRVDGDIRRINTPALSHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPRLAR
FRVNAFVQNRGASAVFRVIPSDIKTLEELGCPRIFQEIAETPRGLVLVTGPTGSGKSTTLAAMINYINANEYGHILTVED
PIEFVHTSQKCLINQREVGPHTMSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKKKEGGRVAAHEVMIGVPAIRNLIRENKIAQMYSAIQTGQQFGMQTLDQCL
QELLKRNIISLPEARMKAAIPDNFRG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=263737 C1H71_RS04960 WP_130105582.1 1142286..1143326(-) (pilT) [Iodobacter fluviatilis strain PCH194]
ATGGAAATCTCCGAACTATTAGCTTTCACCGTGAAAAACAAGGCATCCGATTTACATCTATCGTCTGGATTGCCTCCCAT
GATTCGCGTTGACGGAGATATTCGTCGCATTAATACGCCGGCACTGTCGCATAAAGACGTACACGATATGGTGTACGACA
TCATGAATGACGGCCAGCGCAAGATTTATGAAGATACGCTGGAATGCGATTTTTCTTTCGAAATACCACGCTTAGCCCGC
TTTCGGGTGAATGCCTTTGTGCAAAACCGTGGGGCAAGTGCGGTATTTCGGGTGATTCCATCTGATATTAAAACGCTGGA
AGAACTGGGTTGCCCCCGTATTTTCCAAGAGATTGCCGAAACACCACGTGGCTTGGTGCTGGTAACTGGCCCTACGGGAT
CGGGTAAATCCACCACGCTGGCGGCAATGATTAACTATATCAATGCCAATGAATACGGGCATATCTTAACCGTGGAAGAC
CCGATTGAGTTTGTCCATACCTCGCAAAAATGCCTGATTAACCAGCGGGAAGTTGGGCCACATACCATGAGCTTTTCTAA
CGCTTTGCGCTCAGCATTGCGTGAAGATCCTGACGTAATTCTGGTGGGGGAAATGCGTGATCTAGAAACGATTCGTCTGG
CACTGACTGCCGCTGAAACCGGTCACTTGGTGTTTGGGACGCTACATACCTCCTCAGCTGCTAAAACGATTGATCGGGTG
GTCGATGTGTTTCCAGCAGCAGAAAAAGAAATGGTTCGCTCTATGCTCTCTGAATCATTAAGGGCGGTTATTTCGCAGAC
ATTATTAAAAAAGAAAGAAGGCGGGCGTGTAGCTGCGCATGAAGTCATGATTGGTGTGCCAGCGATTCGAAATTTGATTC
GTGAAAATAAAATTGCTCAGATGTATTCGGCAATTCAAACCGGCCAGCAGTTTGGCATGCAGACTTTAGATCAGTGCTTA
CAAGAGTTATTGAAGCGCAATATTATTTCTTTGCCTGAGGCACGAATGAAAGCGGCCATTCCAGATAATTTTAGAGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G3G6S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.581

99.422

0.751
  pilT Acinetobacter baumannii D1279779

75.581

99.422

0.751
  pilT Acinetobacter baumannii strain A118

75.581

99.422

0.751
  pilT Pseudomonas stutzeri DSM 10701

74.709

99.422

0.743
  pilT Pseudomonas aeruginosa PAK

74.419

99.422

0.74
  pilT Acinetobacter baylyi ADP1

74.128

99.422

0.737
  pilT Legionella pneumophila strain Lp02

73.939

95.376

0.705
  pilT Legionella pneumophila strain ERS1305867

73.939

95.376

0.705
  pilT Neisseria meningitidis 8013

71.091

97.977

0.697
  pilT Neisseria gonorrhoeae MS11

70.796

97.977

0.694
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.399

0.662
  pilT Vibrio cholerae strain A1552

67.953

97.399

0.662
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.711

0.503
  pilU Vibrio cholerae strain A1552

42.486

100

0.425
  pilU Pseudomonas stutzeri DSM 10701

41.04

100

0.41
  pilU Acinetobacter baylyi ADP1

39.769

100

0.399

Multiple sequence alignment