Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C1H71_RS04955 Genome accession   NZ_CP025781
Coordinates   1140886..1142022 (-) Length   378 a.a.
NCBI ID   WP_130105581.1    Uniprot ID   -
Organism   Iodobacter fluviatilis strain PCH194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1135886..1147022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1H71_RS21765 (C1H71_04975) - 1136870..1137997 (+) 1128 WP_130105575.1 methyl-accepting chemotaxis protein -
  C1H71_RS04930 (C1H71_04980) - 1138090..1138695 (-) 606 WP_130105576.1 peroxiredoxin -
  C1H71_RS04935 - 1139040..1139222 (-) 183 WP_130105577.1 hypothetical protein -
  C1H71_RS04940 (C1H71_04985) - 1139410..1140084 (+) 675 WP_130105578.1 methyltransferase -
  C1H71_RS04950 (C1H71_04990) - 1140627..1140878 (-) 252 WP_188053595.1 FimV family protein -
  C1H71_RS04955 (C1H71_04995) pilU 1140886..1142022 (-) 1137 WP_130105581.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1H71_RS04960 (C1H71_05000) pilT 1142286..1143326 (-) 1041 WP_130105582.1 type IV pilus twitching motility protein PilT Machinery gene
  C1H71_RS04965 (C1H71_05005) - 1143505..1144218 (+) 714 WP_188053598.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1H71_RS04970 (C1H71_05010) proC 1144258..1145058 (+) 801 WP_130105584.1 pyrroline-5-carboxylate reductase -
  C1H71_RS04975 (C1H71_05015) - 1145058..1145651 (+) 594 WP_130105585.1 YggT family protein -
  C1H71_RS04980 (C1H71_05020) - 1145804..1146229 (-) 426 WP_130105586.1 FxsA family protein -
  C1H71_RS04985 (C1H71_05025) cutA 1146228..1146572 (+) 345 WP_130105587.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42061.18 Da        Isoelectric Point: 6.5031

>NTDB_id=263736 C1H71_RS04955 WP_130105581.1 1140886..1142022(-) (pilU) [Iodobacter fluviatilis strain PCH194]
MEKEQAAKFMHDLLRHMRGKGASDLFITADFPPAMKIDGKVTPVSNQILSAQHSKELARAIMNDRQAEDFEASKECNFAI
SPGQLGRFRVNAFMQQGHVGMVLRTINSEIPSLGELGLPPVLCDIAMAKRGLVIFVGGTGSGKSTSLAAMIGHRNEHGYD
HIITIEDPIEYVHAHKNCIVTQREVGVDTDSWQAALKNTLRQAPDVILIGEIRDRETMDYAIAFSETGHLCMATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLRSFVSQRLVPHRTGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQALFDHYEAGKITYEDALRNADSVNDLRLSIKLQGAEAKHTDVLSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=263736 C1H71_RS04955 WP_130105581.1 1140886..1142022(-) (pilU) [Iodobacter fluviatilis strain PCH194]
ATGGAAAAAGAACAAGCTGCAAAATTTATGCACGACTTATTGCGGCATATGCGTGGTAAGGGGGCGTCGGATTTATTTAT
CACGGCTGATTTCCCACCAGCTATGAAAATTGATGGCAAAGTGACGCCGGTTTCAAATCAAATTCTTTCTGCACAACACA
GTAAAGAATTAGCGCGGGCGATTATGAATGATCGCCAAGCAGAAGATTTTGAAGCATCCAAAGAGTGTAATTTTGCGATT
AGCCCTGGCCAGCTTGGGCGCTTTCGGGTGAATGCTTTTATGCAGCAAGGCCATGTAGGCATGGTGCTGCGTACGATTAA
CTCTGAAATTCCATCCTTGGGCGAGTTAGGTCTGCCGCCAGTTTTATGCGATATCGCTATGGCCAAACGGGGTTTGGTGA
TTTTTGTGGGCGGTACGGGCTCGGGTAAATCCACGTCGCTAGCGGCGATGATTGGGCATCGTAATGAGCATGGTTACGAT
CACATCATTACCATTGAAGACCCGATTGAATACGTACACGCACATAAAAATTGCATTGTGACTCAGCGTGAAGTAGGTGT
CGATACGGACTCATGGCAGGCCGCGCTGAAAAATACCTTACGCCAAGCACCAGATGTGATTTTAATCGGTGAAATTCGCG
ACCGTGAAACGATGGATTACGCGATTGCTTTCTCTGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAATTCGGCC
AATCAAGCGTTGGATCGGATTATCAATTTCTTTCCAGAAGAGCGCCGCCAGCAGTTATTGATGGATCTATCGCTCAATCT
ACGCTCCTTTGTTTCGCAACGCTTGGTGCCGCATCGCACGGGTAAAGGGCGCGTGGCTGCGGTGGAAGTGATGCTGAATA
GTCCTTTGATTTCTGAGTTGATTTTTAAAGGTGAAGTGCACGAAATCAAAGAAATTATGAAAAAATCGCGTGAGTTAGGC
ATGCAAACTTTTGATCAGGCTTTATTTGATCACTATGAGGCGGGCAAAATTACTTACGAAGATGCTTTGCGCAATGCCGA
TTCGGTGAATGATTTACGCTTGAGCATTAAACTCCAGGGGGCCGAGGCTAAGCATACAGATGTACTTTCTGGCCTTGATC
ACCTCGATATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.533

92.857

0.59
  pilU Acinetobacter baylyi ADP1

58.217

94.974

0.553
  pilU Vibrio cholerae strain A1552

55.952

88.889

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Legionella pneumophila strain ERS1305867

43.849

83.862

0.368
  pilT Legionella pneumophila strain Lp02

43.849

83.862

0.368
  pilT Acinetobacter nosocomialis M2

41.994

87.566

0.368
  pilT Acinetobacter baumannii D1279779

41.994

87.566

0.368
  pilT Acinetobacter baumannii strain A118

41.994

87.566

0.368
  pilT Acinetobacter baylyi ADP1

44.625

81.217

0.362

Multiple sequence alignment