Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WM46_RS15075 Genome accession   NZ_CP025757
Coordinates   3093526..3094506 (-) Length   326 a.a.
NCBI ID   WP_102602686.1    Uniprot ID   A0A2K9PDQ4
Organism   Citrobacter freundii complex sp. CFNIH2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3088526..3099506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM46_RS15045 (WM46_14925) - 3089288..3089788 (+) 501 WP_061076333.1 SprT family zinc-dependent metalloprotease -
  WM46_RS15050 (WM46_14930) endA 3089880..3090587 (+) 708 WP_102602682.1 deoxyribonuclease I -
  WM46_RS15055 (WM46_14935) rsmE 3090667..3091398 (+) 732 WP_102602683.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WM46_RS15060 (WM46_14940) gshB 3091411..3092358 (+) 948 WP_102602684.1 glutathione synthase -
  WM46_RS15065 (WM46_14945) - 3092533..3093096 (+) 564 WP_061076329.1 YqgE/AlgH family protein -
  WM46_RS15070 (WM46_14950) ruvX 3093096..3093512 (+) 417 WP_102602685.1 Holliday junction resolvase RuvX -
  WM46_RS15075 (WM46_14955) pilT 3093526..3094506 (-) 981 WP_102602686.1 type IV pilus twitching motility protein PilT Machinery gene
  WM46_RS15080 (WM46_14960) - 3094524..3095228 (+) 705 WP_102602687.1 YggS family pyridoxal phosphate-dependent enzyme -
  WM46_RS15085 (WM46_14965) - 3095247..3095813 (+) 567 WP_042998061.1 YggT family protein -
  WM46_RS15090 (WM46_14970) yggU 3095810..3096100 (+) 291 WP_044255664.1 DUF167 family protein YggU -
  WM46_RS15095 (WM46_14975) - 3096108..3096701 (+) 594 WP_061076326.1 XTP/dITP diphosphatase -
  WM46_RS15100 (WM46_14980) hemW 3096694..3097830 (+) 1137 WP_102602688.1 radical SAM family heme chaperone HemW -
  WM46_RS15105 (WM46_14985) - 3097863..3098870 (-) 1008 WP_102602689.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35800.87 Da        Isoelectric Point: 7.0663

>NTDB_id=263701 WM46_RS15075 WP_102602686.1 3093526..3094506(-) (pilT) [Citrobacter freundii complex sp. CFNIH2]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTVADITGLLADWLDEGQQAALRQNGQLDFAVSLGDNQRLRA
SAFHQRQGTSLALRLLPTHCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YVYASQRCLIQQREVGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLESDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=263701 WM46_RS15075 WP_102602686.1 3093526..3094506(-) (pilT) [Citrobacter freundii complex sp. CFNIH2]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGGCGCATGGAGCGTGCGCCCTTCACCGTAGCCGACATCACCGGACTGCTGGCGGACTGGCTGGATG
AGGGGCAGCAGGCGGCGTTACGGCAAAACGGGCAACTGGATTTCGCCGTTTCGCTTGGGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTGCGACTGCTGCCGACGCATTGCCCGACGCTGGAAACGCTGGG
GACGCCATCCGTACTGCCCGAACTACTCCGCAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGCAGCGGAAAAT
CAACCACCCTGGCGGCGATGGTCACGTGGCTGAATCAGCATATTGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATGTCTACGCCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGTGGGACTGCACTGCGCCTCATTTGCCACCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGTTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCAATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTGCTGTCGCAGAAACTGGAGTC
CGACAAGCAGGAGGGGCGCGTTGCTCTGTATGAACTGCTGATCAACACCCCGGCGGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCGCAAGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9PDQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.396

97.546

0.423
  pilU Vibrio cholerae strain A1552

43.871

95.092

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383

Multiple sequence alignment