Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NEUT_RS11865 Genome accession   NC_008344
Coordinates   2465042..2466085 (-) Length   347 a.a.
NCBI ID   WP_011635326.1    Uniprot ID   Q0ADM7
Organism   Nitrosomonas eutropha C91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2460042..2471085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEUT_RS11850 (Neut_2335) - 2460803..2462515 (-) 1713 WP_011634833.1 multiheme c-type cytochrome -
  NEUT_RS11855 (Neut_2336) mnmA 2462807..2463892 (+) 1086 WP_011635324.1 tRNA 2-thiouridine(34) synthase MnmA -
  NEUT_RS11860 (Neut_2337) pilU 2463893..2465029 (-) 1137 WP_011635325.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NEUT_RS11865 (Neut_2338) pilT 2465042..2466085 (-) 1044 WP_011635326.1 type IV pilus twitching motility protein PilT Machinery gene
  NEUT_RS11870 (Neut_2339) - 2466280..2466996 (+) 717 WP_011635327.1 YggS family pyridoxal phosphate-dependent enzyme -
  NEUT_RS11875 (Neut_2340) - 2466965..2467234 (-) 270 WP_011635328.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NEUT_RS11880 (Neut_2341) coaD 2467240..2467734 (-) 495 WP_011635329.1 pantetheine-phosphate adenylyltransferase -
  NEUT_RS11885 (Neut_2342) rsmD 2467727..2468272 (-) 546 WP_309147365.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NEUT_RS11890 (Neut_2343) - 2468296..2469597 (-) 1302 WP_011635331.1 pitrilysin family protein -
  NEUT_RS14250 - 2469763..2470152 (-) 390 Protein_2385 transposase -
  NEUT_RS14255 - 2470232..2470820 (-) 589 Protein_2386 IS3 family transposase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38322.13 Da        Isoelectric Point: 7.3026

>NTDB_id=26330 NEUT_RS11865 WP_011635326.1 2465042..2466085(-) (pilT) [Nitrosomonas eutropha C91]
MNIVELLSFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKAYEEHLECDFSFAISGLAR
FRVNAFNTQRGAASVLRTIPSKVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISIAEARTKAMNKDNFRG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=26330 NEUT_RS11865 WP_011635326.1 2465042..2466085(-) (pilT) [Nitrosomonas eutropha C91]
ATGAACATAGTAGAACTACTCTCATTTGTGGTCAAGAATAGCGCTTCTGATCTGCATTTATCTGCTGGCATGCCACCCAT
GATTCGGGTGCATGGAGAGATTCGGCGTATCAATCTTCCCGCACTGGAGCACAAGGATGTGCATGACATGGTGTACGACA
TCATGAACGATAGCCAGCGTAAAGCTTATGAAGAACATCTGGAATGTGATTTTTCATTTGCTATTTCTGGTCTAGCTCGT
TTCCGAGTCAATGCCTTCAACACCCAACGGGGAGCAGCTTCGGTGCTGCGTACCATTCCATCCAAAGTACTAACGCTTGA
AGAGCTGAAAGCACCTAAGATATTTGCCGAGATCGCGCAACAGCCGCGTGGCGTGGTGTTGGTTACGGGGCCAACCGGAT
CTGGCAAGTCAACTACGCTTGCAGCCATGATCAATGATATTAACGAAAATCAGTATGGTCATATCCTGACTCTGGAAGAT
CCGATCGAATTTGTGCATGAAAGCAAAAAATGTCTGATTAACCAGCGTGAAGTCGGGCGAGATACGCACAGTTTCAGCAA
TGCACTGCGCTCTGCCTTACGGGAAGATCCAGATATTATTCTGGTGGGGGAAATGCGGGATTTGGAGACTATCCGCTTGG
CGATGACTGCTGCGGAAACCGGTCATCTGGTTTTCGGCACGCTACATACCAGTTCGGCTGCCAAGACGATTGACCGGATT
ATTGACGTGTTTCCTGCTGAAGAAAAGGAAATGGTACGTGCGATGTTGTCTGAATCGTTGCGTGCAGTTATTTCCCAGGC
GCTGCTGAAGACAAAGGATGGCAAAGGGCGAGTGGCCGCACACGAGATCATGATCGGTACACCGGCGATCCGTAACCTTA
TCCGGGAAGGGAAAGTGGCACAGATGTATTCAGCCATCCAGACCGGGCAGGGAGTCGGGATGCAGACACTTGATCAGAAT
CTGACAGATCTGGTGAAACGCGGTGTTATTTCGATAGCGGAAGCGCGCACCAAAGCGATGAACAAAGATAATTTCCGGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0ADM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.261

99.424

0.778
  pilT Acinetobacter baumannii D1279779

78.261

99.424

0.778
  pilT Acinetobacter baumannii strain A118

78.261

99.424

0.778
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

74.203

99.424

0.738
  pilT Pseudomonas stutzeri DSM 10701

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

72.464

99.424

0.72
  pilT Legionella pneumophila strain ERS1305867

72.464

99.424

0.72
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.486

99.712

0.424
  pilU Vibrio cholerae strain A1552

42.197

99.712

0.421
  pilU Acinetobacter baylyi ADP1

41.21

100

0.412

Multiple sequence alignment