Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NEUT_RS11860 Genome accession   NC_008344
Coordinates   2463893..2465029 (-) Length   378 a.a.
NCBI ID   WP_011635325.1    Uniprot ID   Q0ADM8
Organism   Nitrosomonas eutropha C91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458893..2470029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEUT_RS11840 (Neut_2333) cycA 2458916..2459623 (-) 708 WP_011634831.1 cytochrome c-550 CycA -
  NEUT_RS11845 (Neut_2334) haoB 2459685..2460806 (-) 1122 WP_011635323.1 hydroxylamine oxidation protein HaoB -
  NEUT_RS11850 (Neut_2335) - 2460803..2462515 (-) 1713 WP_011634833.1 multiheme c-type cytochrome -
  NEUT_RS11855 (Neut_2336) mnmA 2462807..2463892 (+) 1086 WP_011635324.1 tRNA 2-thiouridine(34) synthase MnmA -
  NEUT_RS11860 (Neut_2337) pilU 2463893..2465029 (-) 1137 WP_011635325.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NEUT_RS11865 (Neut_2338) pilT 2465042..2466085 (-) 1044 WP_011635326.1 type IV pilus twitching motility protein PilT Machinery gene
  NEUT_RS11870 (Neut_2339) - 2466280..2466996 (+) 717 WP_011635327.1 YggS family pyridoxal phosphate-dependent enzyme -
  NEUT_RS11875 (Neut_2340) - 2466965..2467234 (-) 270 WP_011635328.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NEUT_RS11880 (Neut_2341) coaD 2467240..2467734 (-) 495 WP_011635329.1 pantetheine-phosphate adenylyltransferase -
  NEUT_RS11885 (Neut_2342) rsmD 2467727..2468272 (-) 546 WP_309147365.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NEUT_RS11890 (Neut_2343) - 2468296..2469597 (-) 1302 WP_011635331.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42135.71 Da        Isoelectric Point: 6.6649

>NTDB_id=26329 NEUT_RS11860 WP_011635325.1 2463893..2465029(-) (pilU) [Nitrosomonas eutropha C91]
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQALSPIHTEALARVIMNNKQAAEFETSKECNFAI
NPEGIGRFRVNTFVQQQRTGIVLRTITTKIPDFDDLGLPPVLKEVVMSKRGLVVFVGGTGSGKSTSMAALIGYRNKSSYG
HIITIEDPVEFVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRVAAIEIMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYESGVISYEDALRNADSVNELRLQIKLNGAEAKRIHTETNIENLTIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=26329 NEUT_RS11860 WP_011635325.1 2463893..2465029(-) (pilU) [Nitrosomonas eutropha C91]
ATGGAAAAAGAACAGGCACTTAAATTTATGCATGATTTATTGCGACTGATGCTAAGCAAGAAGGCTTCAGACTTGTTCAT
CACAGCAGGCTATCCTCCGGCGATGAAAATAGATGGAAAAATGACACCGGTCACGCAACAGGCGCTCTCTCCTATACATA
CCGAAGCTCTGGCACGGGTCATCATGAATAATAAGCAGGCGGCAGAGTTTGAGACCAGTAAGGAATGCAATTTTGCGATT
AATCCTGAGGGGATCGGGCGTTTTCGTGTTAATACCTTTGTCCAGCAACAGCGGACGGGCATCGTTTTGCGGACAATTAC
CACAAAGATCCCGGATTTCGATGATCTCGGGTTGCCACCGGTTCTGAAAGAGGTTGTCATGAGCAAACGCGGTCTGGTGG
TTTTTGTCGGTGGAACCGGTTCGGGAAAATCTACCTCGATGGCGGCGTTGATCGGCTATCGCAACAAAAGCAGCTATGGA
CACATCATTACCATTGAAGATCCGGTTGAATTTGTGCATGAGCATATCAACTGCGTCATTACTCAGCGGGAAGTCGGGGT
TGATACGGAATCCTGGGAAGCTGCGCTGAAGAATACGTTACGGCAGGCGCCTGATGTTATTTTGATTGGTGAAATCCGGG
ATCGGGAAACAATGGAACATGCCATTGCCTTTGCCGAGACCGGGCATTTATGCATGGGTACCTTGCACGCAAACAGTGCT
AATCAGGCGTTGGATCGCATTATCAACTTCTTTCCGGAAGAGCGGCGTGCGCAGTTGCTGATGGATTTATCACTGAATAT
GCGGGCATTGGTTGCACAAAGGTTAATTCCCAAAAAATCAGGCAGCGGGCGGGTTGCGGCAATCGAGATAATGCTCAATT
CTCCGTTGATTTCCGATCTGATATTCAAGGGAAATGTGCATGAAATCAAGGAAATCATGAAAAAATCAAGTGAATTGGGG
ATGCAGACCTTTGATATGGCGCTATTTGAACTTTACGAGAGTGGTGTGATTTCCTACGAAGATGCATTACGTAATGCTGA
TTCCGTGAATGAACTGCGACTGCAAATCAAACTGAACGGAGCAGAAGCAAAACGTATACATACAGAGACAAACATCGAAA
ATTTAACCATTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(18-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0ADM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.192

96.561

0.601
  pilU Acinetobacter baylyi ADP1

60.172

92.328

0.556
  pilU Vibrio cholerae strain A1552

56.012

90.212

0.505
  pilT Pseudomonas aeruginosa PAK

44.179

88.624

0.392
  pilT Legionella pneumophila strain Lp02

43.713

88.36

0.386
  pilT Legionella pneumophila strain ERS1305867

43.713

88.36

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

88.624

0.381
  pilT Pseudomonas stutzeri DSM 10701

42.687

88.624

0.378
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

87.302

0.368
  pilT Vibrio cholerae strain A1552

42.121

87.302

0.368
  pilT Acinetobacter baylyi ADP1

41.692

87.566

0.365

Multiple sequence alignment