Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C0099_RS02645 Genome accession   NZ_CP025682
Coordinates   558570..559613 (-) Length   347 a.a.
NCBI ID   WP_102246014.1    Uniprot ID   A0A2I6S3V4
Organism   Pseudazoarcus pumilus strain SY39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 553570..564613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0099_RS02620 (C0099_02620) - 553825..554841 (-) 1017 WP_102246009.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  C0099_RS02625 (C0099_02625) - 554861..555751 (+) 891 WP_102246010.1 SDR family oxidoreductase -
  C0099_RS02630 (C0099_02630) - 555745..556155 (+) 411 WP_102246011.1 CopD family protein -
  C0099_RS02635 (C0099_02635) - 556169..557326 (+) 1158 WP_102246012.1 class I SAM-dependent RNA methyltransferase -
  C0099_RS02640 (C0099_02640) pilU 557357..558490 (-) 1134 WP_102246013.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C0099_RS02645 (C0099_02645) pilT 558570..559613 (-) 1044 WP_102246014.1 type IV pilus twitching motility protein PilT Machinery gene
  C0099_RS02650 (C0099_02650) - 559705..560418 (+) 714 WP_102246015.1 YggS family pyridoxal phosphate-dependent enzyme -
  C0099_RS02655 (C0099_02655) proC 560415..561227 (+) 813 WP_102246016.1 pyrroline-5-carboxylate reductase -
  C0099_RS02660 (C0099_02660) - 561232..561810 (+) 579 WP_102246017.1 YggT family protein -
  C0099_RS02665 (C0099_02665) - 561943..562995 (+) 1053 WP_102245883.1 IS110 family transposase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38735.39 Da        Isoelectric Point: 6.6656

>NTDB_id=263214 C0099_RS02645 WP_102246014.1 558570..559613(-) (pilT) [Pseudazoarcus pumilus strain SY39]
MDITELLAFAVKNKASDLHMSAGLPPMIRVHGDIRRINLPPMEHKDVHAMVYDIMNDSQRKQFEDELECDFSFEIPNLAR
FRVNAFNQNRGASAVFRTIPSKVLTLEELNAPKIFREIADQPRGIVLVTGPTGSGKSTTLAAMVDYVNENQYSHILTIED
PIEFVHTSKRSLINQREVHRDTLGFSNALRSALREDPDVILVGEMRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKDMVRAMLSESLRAVIAQTLLKTKDGTGRVAAHEIMLGTAAIRNLIRENKIAQMYSSIQTGQQLGMQTLDQC
LQDLVRRNIVSVSEARMRAQNKDAFAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=263214 C0099_RS02645 WP_102246014.1 558570..559613(-) (pilT) [Pseudazoarcus pumilus strain SY39]
ATGGACATCACCGAACTGCTCGCGTTTGCGGTCAAGAACAAGGCCTCGGATCTGCACATGTCTGCGGGCCTGCCGCCGAT
GATCCGCGTACACGGCGACATCCGGCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTACACGCGATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGCAGTTCGAGGACGAACTCGAATGCGACTTCTCGTTCGAGATTCCCAACCTCGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCTCGGCCGTGTTCCGCACGATTCCGTCCAAGGTGCTCACGCTCGA
GGAACTGAACGCGCCCAAGATCTTCCGCGAGATCGCCGACCAGCCGCGCGGCATCGTGCTGGTCACCGGGCCCACCGGCT
CGGGCAAATCGACCACGCTCGCGGCCATGGTCGATTACGTCAACGAGAACCAGTACTCGCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACACCTCCAAGCGCAGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCTGGGCTTTTCCAA
CGCACTGCGCTCGGCCCTGCGCGAGGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGGCGCCGAGACCGGCCATCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTTCCGGCCGAGGAAAAGGACATGGTGCGCGCGATGCTGTCCGAATCGCTGCGCGCGGTCATCGCCCAGAC
CCTGCTCAAGACCAAGGACGGCACGGGTCGCGTGGCGGCGCACGAAATCATGCTGGGCACCGCCGCGATCCGCAACCTCA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCCATCCAGACCGGCCAGCAGCTGGGCATGCAGACGCTGGATCAGTGC
CTGCAGGATCTGGTGCGTCGCAATATCGTGTCGGTGTCCGAGGCGCGCATGCGCGCGCAGAACAAGGACGCCTTCGCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I6S3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

71.098

99.712

0.709
  pilT Neisseria gonorrhoeae MS11

70.809

99.712

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

97.983

0.671
  pilT Vibrio cholerae strain A1552

68.529

97.983

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.734

99.712

0.516
  pilU Pseudomonas stutzeri DSM 10701

40.525

98.847

0.401
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

40.179

96.83

0.389

Multiple sequence alignment