Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C0099_RS02640 Genome accession   NZ_CP025682
Coordinates   557357..558490 (-) Length   377 a.a.
NCBI ID   WP_102246013.1    Uniprot ID   A0A2I6S3U8
Organism   Pseudazoarcus pumilus strain SY39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552357..563490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0099_RS02615 (C0099_02615) - 552684..553820 (-) 1137 WP_102246008.1 EAL and HDOD domain-containing protein -
  C0099_RS02620 (C0099_02620) - 553825..554841 (-) 1017 WP_102246009.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  C0099_RS02625 (C0099_02625) - 554861..555751 (+) 891 WP_102246010.1 SDR family oxidoreductase -
  C0099_RS02630 (C0099_02630) - 555745..556155 (+) 411 WP_102246011.1 CopD family protein -
  C0099_RS02635 (C0099_02635) - 556169..557326 (+) 1158 WP_102246012.1 class I SAM-dependent RNA methyltransferase -
  C0099_RS02640 (C0099_02640) pilU 557357..558490 (-) 1134 WP_102246013.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C0099_RS02645 (C0099_02645) pilT 558570..559613 (-) 1044 WP_102246014.1 type IV pilus twitching motility protein PilT Machinery gene
  C0099_RS02650 (C0099_02650) - 559705..560418 (+) 714 WP_102246015.1 YggS family pyridoxal phosphate-dependent enzyme -
  C0099_RS02655 (C0099_02655) proC 560415..561227 (+) 813 WP_102246016.1 pyrroline-5-carboxylate reductase -
  C0099_RS02660 (C0099_02660) - 561232..561810 (+) 579 WP_102246017.1 YggT family protein -
  C0099_RS02665 (C0099_02665) - 561943..562995 (+) 1053 WP_102245883.1 IS110 family transposase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42354.68 Da        Isoelectric Point: 6.2882

>NTDB_id=263213 C0099_RS02640 WP_102246013.1 557357..558490(-) (pilU) [Pseudazoarcus pumilus strain SY39]
MERDQALKFMHDLLRLMVQKNGSDLFVTSGFPPAIKIDGKIIPQSNQVLTPQHTAELARAVMNDRQAAEFEATKECNFAV
APPNIGRFRANAYIQQSRVGLVLRTISQTIPTFEELGLPAILKDIAMSKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEFVHPHKNCIITQREIGIDTDHWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANST
NQALDRIINFFPEDRRQQLLMDLSLNVRALISQRLLPMKERKGRAPAVEIMLNSPLISDLIFKGDVPGIKEVIKRSRELG
MQTFDQSLFDLYEDGLITYEDALRNADSLNDLRLQIKLHSKHGEKDFLSGTENLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=263213 C0099_RS02640 WP_102246013.1 557357..558490(-) (pilU) [Pseudazoarcus pumilus strain SY39]
ATGGAACGCGACCAGGCCCTCAAGTTCATGCACGATCTGCTGCGGCTGATGGTGCAGAAGAACGGCTCGGACCTCTTCGT
CACCTCGGGCTTTCCGCCGGCGATCAAGATCGACGGCAAGATCATTCCGCAATCCAATCAGGTACTCACGCCGCAGCACA
CGGCGGAACTGGCGCGCGCGGTCATGAACGACCGCCAGGCGGCGGAATTCGAAGCCACCAAGGAATGCAACTTCGCCGTC
GCGCCGCCGAACATCGGACGCTTTCGCGCCAACGCCTACATCCAGCAAAGCCGCGTCGGCCTGGTGCTGCGCACCATCTC
GCAGACCATACCGACCTTCGAGGAACTGGGCCTGCCGGCCATCCTCAAGGACATCGCGATGAGCAAGCGCGGGCTGGTGA
TTTTCGTCGGCGGCACCGGCACCGGCAAGACCACCTCGCTGGCGGCGATGGTGGATTTCCGCAACGAGCACTCGTACGGC
CACATCATCACCGTCGAGGATCCGATCGAATTCGTCCATCCGCACAAGAACTGCATCATCACGCAGCGCGAGATCGGCAT
CGACACCGACCACTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCACC
AACCAGGCGCTCGACCGCATCATCAACTTCTTTCCTGAAGACCGCCGCCAGCAGTTGCTGATGGACTTGTCGCTCAACGT
GCGCGCACTGATCTCGCAGCGCCTGCTGCCGATGAAGGAGCGCAAGGGCCGCGCACCGGCCGTGGAAATCATGCTCAACT
CGCCGCTGATCTCGGATCTGATCTTCAAGGGCGACGTGCCCGGCATCAAGGAGGTCATCAAGCGTTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGACCTCTACGAGGATGGTCTCATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACTCCAAGCACGGCGAGAAGGATTTCCTGTCGGGCACGGAGAATC
TGGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I6S3U8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.89

96.552

0.578
  pilU Acinetobacter baylyi ADP1

58.453

92.573

0.541
  pilU Vibrio cholerae strain A1552

53.186

95.756

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

88.859

0.39
  pilT Acinetobacter baylyi ADP1

43.62

89.39

0.39
  pilT Acinetobacter nosocomialis M2

42.9

87.798

0.377
  pilT Acinetobacter baumannii D1279779

42.9

87.798

0.377
  pilT Acinetobacter baumannii strain A118

42.9

87.798

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

87.533

0.366
  pilT Vibrio cholerae strain A1552

41.818

87.533

0.366
  pilT Legionella pneumophila strain Lp02

41.088

87.798

0.361
  pilT Legionella pneumophila strain ERS1305867

41.088

87.798

0.361
  pilT Neisseria gonorrhoeae MS11

39.883

90.451

0.361
  pilT Neisseria meningitidis 8013

39.883

90.451

0.361
  pilT Pseudomonas stutzeri DSM 10701

40.597

88.859

0.361
  pilT Pseudomonas aeruginosa PAK

40.597

88.859

0.361

Multiple sequence alignment