Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C0119_RS11555 Genome accession   NZ_CP025618
Coordinates   2391703..2392824 (+) Length   373 a.a.
NCBI ID   WP_008305547.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain SGAir0122     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2386703..2397824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0119_RS11540 (C0119_10760) - 2388670..2389776 (+) 1107 WP_108675840.1 acyltransferase family protein -
  C0119_RS11545 (C0119_10755) - 2389823..2390509 (-) 687 WP_008305552.1 YggS family pyridoxal phosphate-dependent enzyme -
  C0119_RS11550 (C0119_10750) pilT 2390633..2391670 (+) 1038 WP_008305550.1 type IV pilus twitching motility protein PilT Machinery gene
  C0119_RS11555 (C0119_10745) pilU 2391703..2392824 (+) 1122 WP_008305547.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C0119_RS11560 (C0119_10740) fur 2392899..2393336 (-) 438 WP_004811125.1 ferric iron uptake transcriptional regulator -
  C0119_RS11565 (C0119_10735) - 2393449..2393847 (+) 399 WP_008305544.1 outer membrane protein assembly factor BamE -
  C0119_RS11570 (C0119_10730) - 2393957..2394283 (-) 327 WP_108675839.1 RnfH family protein -
  C0119_RS11575 (C0119_10725) - 2394283..2395362 (-) 1080 WP_108675838.1 hypothetical protein -
  C0119_RS11580 (C0119_10720) - 2395501..2395956 (-) 456 WP_108675837.1 bacteriohemerythrin -
  C0119_RS11585 (C0119_10715) - 2396233..2397081 (-) 849 WP_017401317.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41437.62 Da        Isoelectric Point: 6.8885

>NTDB_id=263062 C0119_RS11555 WP_008305547.1 2391703..2392824(+) (pilU) [Acinetobacter schindleri strain SGAir0122]
MDFNGLLDYMVAQKASDLFITADVEPSIKVNGSIVPIGSVKLPGPAVGQILHSIMTEKQRKEFADTRECNFAITNPSKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDDLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAMVAQQLIPTIDGTGRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGAHLMNAGSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=263062 C0119_RS11555 WP_008305547.1 2391703..2392824(+) (pilU) [Acinetobacter schindleri strain SGAir0122]
ATGGATTTTAACGGCTTGCTCGACTATATGGTGGCGCAAAAGGCATCGGATCTGTTCATTACTGCAGATGTCGAACCTTC
AATTAAAGTGAATGGCTCAATTGTGCCGATTGGATCAGTCAAATTACCTGGACCAGCAGTAGGCCAGATTCTGCATTCAA
TTATGACGGAAAAACAGCGCAAAGAATTTGCCGATACACGTGAGTGTAACTTTGCGATTACCAATCCCAGCAAATCAGCA
CGTTTCCGTGTCAGTGCATTTCAGCAGCGTGATCAGCCGGGTATGGTTCTACGTCGTATTGAAACCGTCATTCCTACTAT
GGATGATTTAAAGTTACCGCCAATTCTGAAAGAACTGGCGATGACCAAGCGCGGGATTATCATCTTCGTGGGCGCAACAG
GTACCGGTAAATCGACTTCCCTTGCCTCTTTGATTGGTTATCGTAATGAAAATTCCAAAGGTCATATCATCACCATCGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGTTGTATCATCACCCAGCGTGAAGTCGGGATCGATACTGACTCCTTTGA
AGTGGCATTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCCCGTGAAACCATGGACT
ATGCTATTGCCTTTGCGGAAACCGGTCACCTGGTATTTGCGACCTTGCACGCCAATAACGCCAACCAGGCCATTGACCGG
ATTATTCATTTCTTTGAAGCAGACCGCCATAACCAGCTGTTTATGGACTTGTCTTTAAACCTGAAAGCGATGGTGGCACA
GCAGCTGATCCCAACCATTGATGGTACAGGCCGTCGTGCCGCAATTGAGATCCTGATCAACTCACCTTTAGTTTCAGATC
TGATCCGTAAAGGTGATGTGCATGAAATTAAAGACTTGATGAAGCGTTCACGTGAACTTGGAATGCAGACCTTTGACCAG
GCACTGTATGACCTGTACAAGGCCAAACAGATCAGCTATAAGGATGCGTTGAAACATGCCGATTCTCCAAACGATCTGCG
TCTGCAAATCAAGTTATCCGAAGAAGGTGGTGCACATCTGATGAATGCCGGCAGCAATATTACCTTTGATGGACAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.28

100

0.743
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.464

0.681
  pilU Vibrio cholerae strain A1552

57.55

94.102

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.322

97.319

0.402
  pilT Legionella pneumophila strain Lp02

43.75

90.08

0.394
  pilT Legionella pneumophila strain ERS1305867

43.75

90.08

0.394
  pilT Acinetobacter nosocomialis M2

42.274

91.957

0.389
  pilT Acinetobacter baumannii D1279779

42.274

91.957

0.389
  pilT Acinetobacter baumannii strain A118

42.274

91.957

0.389
  pilT Pseudomonas aeruginosa PAK

40.751

92.761

0.378
  pilT Neisseria meningitidis 8013

40.462

92.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.462

92.761

0.375
  pilT Acinetobacter baylyi ADP1

40.233

91.957

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.884

92.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.102

0.367
  pilT Vibrio cholerae strain A1552

39.031

94.102

0.367

Multiple sequence alignment