Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHEWMR4_RS06285 Genome accession   NC_008321
Coordinates   1397035..1398072 (+) Length   345 a.a.
NCBI ID   WP_011621982.1    Uniprot ID   -
Organism   Shewanella sp. MR-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1392035..1403072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWMR4_RS06255 (Shewmr4_1188) rdgB 1393045..1393662 (-) 618 WP_011621976.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SHEWMR4_RS06260 (Shewmr4_1189) - 1393823..1394257 (-) 435 WP_011621977.1 DUF4426 domain-containing protein -
  SHEWMR4_RS06265 (Shewmr4_1190) yggU 1394326..1394616 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  SHEWMR4_RS06270 (Shewmr4_1191) - 1394616..1395164 (-) 549 WP_011621979.1 YggT family protein -
  SHEWMR4_RS06275 (Shewmr4_1192) proC 1395240..1396058 (-) 819 WP_011621980.1 pyrroline-5-carboxylate reductase -
  SHEWMR4_RS21180 - 1396155..1396286 (+) 132 WP_264622860.1 hypothetical protein -
  SHEWMR4_RS06280 (Shewmr4_1193) - 1396283..1396999 (-) 717 WP_011621981.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEWMR4_RS06285 (Shewmr4_1194) pilT 1397035..1398072 (+) 1038 WP_011621982.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEWMR4_RS06290 (Shewmr4_1195) pilU 1398082..1399194 (+) 1113 WP_011621983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEWMR4_RS06295 (Shewmr4_1196) - 1399371..1399904 (+) 534 WP_041408729.1 glutathione peroxidase -
  SHEWMR4_RS06300 (Shewmr4_1197) hemH 1399974..1400957 (+) 984 WP_011621985.1 ferrochelatase -
  SHEWMR4_RS06305 (Shewmr4_1198) ruvX 1401019..1401444 (-) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  SHEWMR4_RS06310 (Shewmr4_1199) - 1401475..1402038 (-) 564 WP_011621987.1 YqgE/AlgH family protein -
  SHEWMR4_RS06315 (Shewmr4_1200) yciH 1402107..1402436 (-) 330 WP_011621988.1 stress response translation initiation inhibitor YciH -
  SHEWMR4_RS06320 (Shewmr4_1201) - 1402586..1402900 (-) 315 WP_011621989.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38340.02 Da        Isoelectric Point: 6.9097

>NTDB_id=26270 SHEWMR4_RS06285 WP_011621982.1 1397035..1398072(+) (pilT) [Shewanella sp. MR-4]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=26270 SHEWMR4_RS06285 WP_011621982.1 1397035..1398072(+) (pilT) [Shewanella sp. MR-4]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATAAACCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAGCATTTAGAAATCGACTTTTCGTTCGAAGTCCCTAATTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCCCGCGGCGCTGCGGCGGTGTTTCGTACCATTCCCAGCGAAATTTTGTCGCTCGA
GCAGTTAGGGGCGCCTGAGATTTTTAAGAAAATTTCCAGCTTTCCCCGCGGCTTAGTGCTTGTTACTGGGCCTACAGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAGAACCGCCACGACCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTACATCAGAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGTTTTAACGC
GGCGCTGCGTAGCGCACTGCGTGAAGACCCTGACGTAATCCTCGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CGATGACGGCGGCCGAAACGGGTCACTTAGTCTTTGGTACCTTGCACACCACCTCGGCGGCTAAGACCATCGACCGTGTG
GTTGACGTTTTCCCTGCCGGTGAAAAGGACATGGTGCGTACCATGTTATCTGAATCATTACAGGCGGTTATTTCGCAAAC
CCTGATTAAGAAAATCGGTGGTGGCCGTGTGGCTGCCCACGAAATCATGATGGGTACTCCCGCTATCCGTAACCTTATCC
GTGAAGATAAAGTGGCACAGATGTACTCAGCCATTCAAACGGGGATGGCCCATGGCATGCAAACGCTCGAACAGTGTCTG
CAAAACTTAGTGAACCGTGGCCTCATCACCCGTGAGGATGCCATGGCGAAGAGCTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.681

0.704
  pilT Vibrio cholerae strain A1552

72.107

97.681

0.704
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment