Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SHEWMR4_RS06290 Genome accession   NC_008321
Coordinates   1398082..1399194 (+) Length   370 a.a.
NCBI ID   WP_011621983.1    Uniprot ID   -
Organism   Shewanella sp. MR-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1393082..1404194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWMR4_RS06260 (Shewmr4_1189) - 1393823..1394257 (-) 435 WP_011621977.1 DUF4426 domain-containing protein -
  SHEWMR4_RS06265 (Shewmr4_1190) yggU 1394326..1394616 (-) 291 WP_011621978.1 DUF167 family protein YggU -
  SHEWMR4_RS06270 (Shewmr4_1191) - 1394616..1395164 (-) 549 WP_011621979.1 YggT family protein -
  SHEWMR4_RS06275 (Shewmr4_1192) proC 1395240..1396058 (-) 819 WP_011621980.1 pyrroline-5-carboxylate reductase -
  SHEWMR4_RS21180 - 1396155..1396286 (+) 132 WP_264622860.1 hypothetical protein -
  SHEWMR4_RS06280 (Shewmr4_1193) - 1396283..1396999 (-) 717 WP_011621981.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEWMR4_RS06285 (Shewmr4_1194) pilT 1397035..1398072 (+) 1038 WP_011621982.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEWMR4_RS06290 (Shewmr4_1195) pilU 1398082..1399194 (+) 1113 WP_011621983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEWMR4_RS06295 (Shewmr4_1196) - 1399371..1399904 (+) 534 WP_041408729.1 glutathione peroxidase -
  SHEWMR4_RS06300 (Shewmr4_1197) hemH 1399974..1400957 (+) 984 WP_011621985.1 ferrochelatase -
  SHEWMR4_RS06305 (Shewmr4_1198) ruvX 1401019..1401444 (-) 426 WP_011621986.1 Holliday junction resolvase RuvX -
  SHEWMR4_RS06310 (Shewmr4_1199) - 1401475..1402038 (-) 564 WP_011621987.1 YqgE/AlgH family protein -
  SHEWMR4_RS06315 (Shewmr4_1200) yciH 1402107..1402436 (-) 330 WP_011621988.1 stress response translation initiation inhibitor YciH -
  SHEWMR4_RS06320 (Shewmr4_1201) - 1402586..1402900 (-) 315 WP_011621989.1 hypothetical protein -
  SHEWMR4_RS06325 (Shewmr4_1202) - 1403080..1403640 (+) 561 WP_011621990.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41187.42 Da        Isoelectric Point: 6.3725

>NTDB_id=26271 SHEWMR4_RS06290 WP_011621983.1 1398082..1399194(+) (pilU) [Shewanella sp. MR-4]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYIDGEISYADALHHADSPNDLRLMIKLQNKEPTSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=26271 SHEWMR4_RS06290 WP_011621983.1 1398082..1399194(+) (pilU) [Shewanella sp. MR-4]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCTAG
CGCCAAAATCGATGGTGAGTTACGTCCGTTAGCCGAGAGTGCCTTCACGCCCGCGCAATCTTTGGATTTTGTCGAGTCCG
TGATGACCGAGGCACAAAAGAAGGAGTTTCACACGACTCGCGAGTGTAACTTTGCTTTTGCGGTTAAGGATTTAGGTCGT
TTCCGCGTCAGCGCATTCTGGCAGCGTGAATCTCCTGGATGCGTAATGCGCCGGATTGAGACCAAGATCCCTGAGGTAGA
AGACTTAAAACTGCCACCGATTTTAAAAGATCTGGTGATGAGTAAACGGGGTCTTATCATCATGGTTGGGGGAACGGGAA
CCGGTAAGTCGACCTCCTTGGCGGCGTTAGTGGGTTATCGTAATGCCCATGCCCGTGGTCATATCCTCACCATCGAAGAC
CCGGTGGAATTTGTGCACGACCATCGCAAAAGCATCATCACTCAACGTGAAGTGGGTATAGATACAGAATCCTTTGATGC
GGCGCTGAAGAGTTCGCTGCGTCAGGCGCCCGATGTGATTTTGATTGGTGAGATCCGTACTCAAGAAACCATGGAGTTTG
CGCTTTCTTTCGCCGAAACGGGTCACCTCTGTATGGCGACACTGCACGCGAACAACGCTAACCAAGCGTTAGATCGGATC
ATGCACTTAGTGCCAGAGAGTAAACACCAGCAGTTGTTATTCGACTTGTCACTCAACCTGCGCGGCATTGTGGCGCAGCA
ACTGGTGCCTAAAGTCGATGGCACAGGACGACGGGCGGCGATTGAAGTCTTGATCAATACGCCACGTGTCGCGAGCTTGA
TTGCTAAAAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAACAGGGCATGCAGACCTTCGACCAAGCC
TTGCTGCAACTCTATATCGATGGGGAAATCAGCTATGCCGATGCGCTTCACCATGCTGACTCGCCAAACGACTTACGTTT
AATGATCAAACTGCAAAATAAAGAGCCTACCAGTTCAAGCTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

59.189

100

0.592
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

89.189

0.386
  pilT Vibrio cholerae strain A1552

43.333

89.189

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

41.358

87.568

0.362
  pilT Legionella pneumophila strain ERS1305867

41.358

87.568

0.362

Multiple sequence alignment