Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RXYL_RS05925 Genome accession   NC_008148
Coordinates   1190865..1191968 (-) Length   367 a.a.
NCBI ID   WP_011564149.1    Uniprot ID   Q1AWU7
Organism   Rubrobacter xylanophilus DSM 9941     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1185865..1196968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RXYL_RS05900 (Rxyl_1160) - 1187243..1187866 (-) 624 WP_011564144.1 PulJ/GspJ family protein -
  RXYL_RS05905 (Rxyl_1161) - 1187863..1188288 (-) 426 WP_011564145.1 type IV pilus modification PilV family protein -
  RXYL_RS05910 (Rxyl_1162) - 1188281..1188862 (-) 582 WP_011564146.1 pilus assembly FimT family protein -
  RXYL_RS05915 (Rxyl_1163) - 1188871..1189644 (-) 774 WP_011564147.1 sigma-70 family RNA polymerase sigma factor -
  RXYL_RS05920 (Rxyl_1164) pilC 1189653..1190864 (-) 1212 WP_011564148.1 type II secretion system F family protein Machinery gene
  RXYL_RS05925 (Rxyl_1165) pilT 1190865..1191968 (-) 1104 WP_011564149.1 type IV pilus twitching motility protein PilT Machinery gene
  RXYL_RS05930 (Rxyl_1166) - 1191965..1193683 (-) 1719 WP_011564150.1 GspE/PulE family protein -
  RXYL_RS05935 (Rxyl_1167) - 1193949..1195064 (+) 1116 WP_049761477.1 type IV pilus twitching motility protein PilT -
  RXYL_RS05940 (Rxyl_1168) - 1195141..1196241 (+) 1101 WP_041328140.1 aminopeptidase -
  RXYL_RS05945 (Rxyl_1169) - 1196246..1196485 (+) 240 WP_011564153.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 39856.32 Da        Isoelectric Point: 6.7811

>NTDB_id=25987 RXYL_RS05925 WP_011564149.1 1190865..1191968(-) (pilT) [Rubrobacter xylanophilus DSM 9941]
MSGMVAENGLGDFLLEMLEAGASDLHLTAGSPPAVRVDGEVRPLDHPPLTPVATRDLVYDILTDEQRRRLENDWELDFSY
SVPGAARFRVNVYFQRGSLGAAFRAIPHEIKGLGELGLPEAVEGLTEKPRGLVLVTGPTGSGKSTTLAAMIDRINETRSG
HIMSIEDPIEFLHRHKRCIVNQREVSSDTRSFAAALRHVLRQDPDVILVGEMRDLETISLAVTAAETGHLVFGTLHTQDA
PQTVDRIVDVFPPHQQAQVRAQLANALQGIVTQTLLRRRDGRGRVVACEVLVATPGVRNLIREGKNHQLYSAMQTGGKFG
MQTLDAALAGLVRRGVVSPEEAGRHASNPDELRRLAGAYAGGARGVF

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=25987 RXYL_RS05925 WP_011564149.1 1190865..1191968(-) (pilT) [Rubrobacter xylanophilus DSM 9941]
ATGAGCGGCATGGTGGCGGAGAACGGGCTCGGGGACTTCCTGCTTGAGATGCTCGAGGCCGGGGCCAGCGACCTGCACCT
CACGGCGGGCAGCCCGCCGGCGGTGCGCGTGGACGGCGAGGTGCGGCCTCTGGACCACCCGCCGCTCACCCCGGTGGCCA
CCCGCGACCTCGTCTACGACATCCTCACCGACGAGCAGCGGCGGCGGCTGGAGAACGACTGGGAGCTGGACTTCTCCTAC
TCCGTCCCCGGCGCGGCCCGCTTCCGGGTCAACGTCTACTTCCAGCGGGGCTCGCTCGGGGCCGCCTTCCGGGCCATCCC
CCACGAGATAAAGGGCCTCGGCGAGCTGGGGCTCCCCGAGGCCGTGGAGGGGCTCACCGAGAAGCCGCGCGGGCTCGTGC
TCGTCACCGGGCCCACCGGCAGCGGCAAGTCCACCACGCTCGCCGCCATGATCGACCGCATCAACGAGACCCGCAGCGGC
CACATCATGAGCATCGAGGACCCCATAGAGTTCCTGCACCGGCACAAGCGCTGCATCGTCAACCAGCGGGAGGTCTCCTC
CGACACCCGCAGCTTCGCCGCGGCGCTCCGGCACGTGCTGCGGCAGGACCCGGACGTGATCCTGGTGGGGGAGATGCGGG
ACCTCGAGACCATCTCGCTCGCCGTCACCGCCGCGGAGACCGGCCACCTGGTCTTCGGGACGCTCCACACCCAGGACGCC
CCGCAGACCGTGGACCGCATCGTGGACGTCTTCCCGCCCCACCAGCAGGCCCAGGTGCGCGCGCAGCTGGCCAACGCGCT
GCAGGGCATCGTCACCCAGACGCTCCTCAGGCGGCGCGACGGCCGCGGGCGGGTGGTGGCCTGCGAGGTGCTCGTGGCCA
CCCCGGGGGTGCGCAACCTCATCCGGGAGGGGAAGAACCACCAGCTCTACTCCGCGATGCAGACCGGCGGCAAGTTCGGG
ATGCAGACGCTCGACGCCGCGCTCGCGGGCCTCGTCCGCCGGGGCGTGGTCTCCCCCGAGGAGGCCGGGCGGCACGCCTC
GAACCCCGACGAGCTGCGGCGGCTCGCCGGGGCCTACGCGGGCGGCGCGCGGGGGGTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1AWU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.085

96.458

0.531
  pilT Pseudomonas aeruginosa PAK

57.831

90.463

0.523
  pilT Acinetobacter nosocomialis M2

56.716

91.281

0.518
  pilT Acinetobacter baumannii strain A118

56.716

91.281

0.518
  pilT Acinetobacter baumannii D1279779

56.716

91.281

0.518
  pilT Pseudomonas stutzeri DSM 10701

56.024

90.463

0.507
  pilT Acinetobacter baylyi ADP1

55.589

90.191

0.501
  pilT Legionella pneumophila strain ERS1305867

52.802

92.371

0.488
  pilT Legionella pneumophila strain Lp02

52.802

92.371

0.488
  pilT Neisseria meningitidis 8013

53.172

90.191

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.495

89.646

0.48
  pilT Vibrio cholerae strain A1552

53.495

89.646

0.48
  pilT Neisseria gonorrhoeae MS11

52.87

90.191

0.477
  pilU Pseudomonas stutzeri DSM 10701

46.291

91.826

0.425
  pilU Vibrio cholerae strain A1552

42.978

97.003

0.417
  pilU Acinetobacter baylyi ADP1

40

94.005

0.376

Multiple sequence alignment