Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RMET_RS14715 Genome accession   NC_007973
Coordinates   3191030..3192073 (-) Length   347 a.a.
NCBI ID   WP_008641940.1    Uniprot ID   A0A132HD11
Organism   Cupriavidus metallidurans CH34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3186030..3197073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMET_RS14685 (Rmet_2930) - 3187010..3187396 (+) 387 WP_008641946.1 CidA/LrgA family protein -
  RMET_RS14690 (Rmet_2931) - 3187393..3188118 (+) 726 WP_008641945.1 LrgB family protein -
  RMET_RS14695 (Rmet_2932) - 3188204..3188584 (+) 381 WP_017514351.1 VOC family protein -
  RMET_RS14700 (Rmet_2933) - 3188604..3189296 (+) 693 WP_011517476.1 YafY family protein -
  RMET_RS14705 (Rmet_2934) - 3189323..3189817 (-) 495 WP_008641942.1 glutathione peroxidase -
  RMET_RS14710 (Rmet_2935) pilU 3189873..3191012 (-) 1140 WP_029307174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RMET_RS14715 (Rmet_2936) pilT 3191030..3192073 (-) 1044 WP_008641940.1 type IV pilus twitching motility protein PilT Machinery gene
  RMET_RS14720 (Rmet_2937) - 3192137..3192826 (+) 690 WP_011517478.1 YggS family pyridoxal phosphate-dependent enzyme -
  RMET_RS14725 (Rmet_2938) proC 3192912..3193733 (+) 822 WP_011517479.1 pyrroline-5-carboxylate reductase -
  RMET_RS14730 (Rmet_2939) ubiA 3193762..3194646 (-) 885 WP_011517480.1 4-hydroxybenzoate octaprenyltransferase -
  RMET_RS14735 (Rmet_2940) - 3194761..3195246 (-) 486 WP_008641932.1 Dps family protein -
  RMET_RS14740 (Rmet_6566) - 3195332..3195523 (-) 192 WP_029307173.1 hypothetical protein -
  RMET_RS14745 (Rmet_2941) - 3195691..3196647 (-) 957 WP_011517481.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38396.21 Da        Isoelectric Point: 6.9771

>NTDB_id=25791 RMET_RS14715 WP_008641940.1 3191030..3192073(-) (pilT) [Cupriavidus metallidurans CH34]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGMINYSDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=25791 RMET_RS14715 WP_008641940.1 3191030..3192073(-) (pilT) [Cupriavidus metallidurans CH34]
ATGGACATCGCGCAGCTATTGGCTTTTGCGGTCAAGAACAAGGCATCCGATCTCCACCTCTCGGCTGACATGCCGCCGAT
GGTGCGTATTCACGGGGATATGCGCCGCATCAACGTCGCGGCAATGACGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTTCAGCGCAAGTCTTATGAAGAGCGCCTTGAGGTCGATTTCTCCTTCGAGATCTCTGGTCTGTCCCGT
TTCCGGGTCAACGCCTATACCACCCAGCGCGGCGCGGCCGCGGTGTTCCGGACAATTCCGTCGAAGGTCCTGACGCTCGA
GGACCTGCGCGCGCCCGCCGTGTTCGCCGATCTGTGCATGAAGCCGCGTGGCCTGGTGCTGGTCACCGGGCCCACGGGCT
CGGGCAAGTCGACCACGCTCGCCGCGATGGTCGACCATCGCAACGACAACGACATGGGTCACATCCTCACGGTGGAGGAT
CCAATCGAATTCGTGCATCAGTCCAAGAAGAGTCTGATCAACCAGCGCGAGCTTGGCCCTCACACCCATTCGTTCGCCAA
CGCGCTGCGCTCGGCACTGCGCGAGGACCCGGACGTGGTTCTGGTGGGCGAATTGCGTGACCTGGAAACGATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTTTTCGCCACGCTGCACACGAGTTCGGCCGCCAAGACGATTGACCGTGTG
GTCGACGTGTTCCCGCCGGAAGAAAAGGACATGGTGCGAACCATGCTGTCGGAGTCGCTCGAGGCCGTGATCTCGCAGAC
GCTGCTCAAGACACGCGACGGCAACGGTCGGACGGCGGCACATGAAATCATGATCGCCACGCCTGCGATTCGCCACCTGA
TCCGCGAAAACAAGATCGCGCAGATGTACTCGATGATGCAGACGTCGAGCGGCCTGGGCATGCAGACGCTGGACCAGTGC
CTGAGCGACCTGATCAAGCGGGGCATGATCAACTACAGCGACGCGCGGGCGATTGCGAAAAATCCCGACGCCTTCATGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A132HD11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.387

99.712

0.712
  pilT Acinetobacter baumannii D1279779

71.387

99.712

0.712
  pilT Acinetobacter baumannii strain A118

71.387

99.712

0.712
  pilT Pseudomonas stutzeri DSM 10701

69.565

99.424

0.692
  pilT Acinetobacter baylyi ADP1

69.275

99.424

0.689
  pilT Pseudomonas aeruginosa PAK

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

66.86

99.135

0.663
  pilT Neisseria meningitidis 8013

66.86

99.135

0.663
  pilT Legionella pneumophila strain Lp02

66.087

99.424

0.657
  pilT Legionella pneumophila strain ERS1305867

66.087

99.424

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

99.424

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Vibrio cholerae strain A1552

40

96.542

0.386

Multiple sequence alignment