Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RMET_RS14710 Genome accession   NC_007973
Coordinates   3189873..3191012 (-) Length   379 a.a.
NCBI ID   WP_029307174.1    Uniprot ID   -
Organism   Cupriavidus metallidurans CH34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3184873..3196012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMET_RS14680 (Rmet_2929) glcF 3185602..3186873 (+) 1272 WP_011517474.1 glycolate oxidase subunit GlcF -
  RMET_RS14685 (Rmet_2930) - 3187010..3187396 (+) 387 WP_008641946.1 CidA/LrgA family protein -
  RMET_RS14690 (Rmet_2931) - 3187393..3188118 (+) 726 WP_008641945.1 LrgB family protein -
  RMET_RS14695 (Rmet_2932) - 3188204..3188584 (+) 381 WP_017514351.1 VOC family protein -
  RMET_RS14700 (Rmet_2933) - 3188604..3189296 (+) 693 WP_011517476.1 YafY family protein -
  RMET_RS14705 (Rmet_2934) - 3189323..3189817 (-) 495 WP_008641942.1 glutathione peroxidase -
  RMET_RS14710 (Rmet_2935) pilU 3189873..3191012 (-) 1140 WP_029307174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RMET_RS14715 (Rmet_2936) pilT 3191030..3192073 (-) 1044 WP_008641940.1 type IV pilus twitching motility protein PilT Machinery gene
  RMET_RS14720 (Rmet_2937) - 3192137..3192826 (+) 690 WP_011517478.1 YggS family pyridoxal phosphate-dependent enzyme -
  RMET_RS14725 (Rmet_2938) proC 3192912..3193733 (+) 822 WP_011517479.1 pyrroline-5-carboxylate reductase -
  RMET_RS14730 (Rmet_2939) ubiA 3193762..3194646 (-) 885 WP_011517480.1 4-hydroxybenzoate octaprenyltransferase -
  RMET_RS14735 (Rmet_2940) - 3194761..3195246 (-) 486 WP_008641932.1 Dps family protein -
  RMET_RS14740 (Rmet_6566) - 3195332..3195523 (-) 192 WP_029307173.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42559.97 Da        Isoelectric Point: 6.6193

>NTDB_id=25790 RMET_RS14710 WP_029307174.1 3189873..3191012(-) (pilU) [Cupriavidus metallidurans CH34]
MLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECNFA
IAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=25790 RMET_RS14710 WP_029307174.1 3189873..3191012(-) (pilU) [Cupriavidus metallidurans CH34]
ATGCTCGACCGCGAATCCGCTGCAAAGTACATCAACGAAATGCTGGGGCTGATGGTCAAGAACCGTGGCTCCGATCTGTT
CATCACTGCGGAATTTCCTCCGGCCGTCAAGGTCGACGGCAAGATCACGCCGGTTTCCCAGCAGCCGTTGAACCCGACCC
ACGCGCTTACCCTGGTGTGCTCGATCATGAACGAGCGCCAGACCAAGGAGTTCGAGGACACCCGCGAATGCAATTTCGCG
ATCGCCGTGCCGGACGTCGGACGTTTCCGTGTCTCGGCCTTCATCCAGCAAGGCCGCGCGGGCATGGTGCTGCGGACCAT
CAACACCCGCATTCCATCGGTCGAAGACCTGGACCTGCCGCCGACGCTGCATGAGATCGTGATGTCCAAGCGCGGTCTGG
TGATCGTCACCGGCGCCACTGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGCTCGACCACCGCAACGCGCATTCGTAC
GGCCACATCATCACGATCGAGGACCCGATCGAATACGTCCACGCGCATCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
GATCGACACCGAGTCGTGGCACGTCGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCGATGCAGTACGCGGAAACGGGCCACCTTTGCCTGGCCACGCTGCACGCGAACAAC
GCCAATCAGGCGATCGACCGTATCGTCAACTTCTTCCCAGAGGAAAAGCGCCAGCAGCTTCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATTTCGCAGCGCCTGCTGCCGCGTGCCGGCCGCAAGGGCCGCGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTAGTGGCTGACCTGATCTTCAAGGGCGAGATTCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGATCTCGTTCGACCAAGCGCTGTTCGAACTCTACGAGACGGAGAAGATCACGTACGAAGACGCGCTCAAGAACGC
TGACTCGCTCAACGACCTGCGCCTGATGATCAAACTCCACAGCAAACGCCATCGCGACAACGATCTCGGCGCCGGCACCG
AACACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.857

92.348

0.58
  pilU Acinetobacter baylyi ADP1

59.143

92.348

0.546
  pilU Vibrio cholerae strain A1552

54.545

95.778

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Legionella pneumophila strain Lp02

44.444

87.863

0.39
  pilT Legionella pneumophila strain ERS1305867

44.444

87.863

0.39
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter nosocomialis M2

44.514

84.169

0.375
  pilT Acinetobacter baumannii D1279779

44.514

84.169

0.375
  pilT Acinetobacter baumannii strain A118

44.514

84.169

0.375
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.641

0.367
  pilT Vibrio cholerae strain A1552

43.849

83.641

0.367

Multiple sequence alignment