Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CSAL_RS15460 Genome accession   NC_007963
Coordinates   3402348..3403388 (-) Length   346 a.a.
NCBI ID   WP_011508343.1    Uniprot ID   Q1QT11
Organism   Chromohalobacter israelensis DSM 3043     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3397348..3408388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSAL_RS15445 (Csal_3050) tyrS 3398731..3399927 (-) 1197 WP_011508340.1 tyrosine--tRNA ligase -
  CSAL_RS15450 (Csal_3051) - 3400028..3401143 (+) 1116 WP_011508341.1 anhydro-N-acetylmuramic acid kinase -
  CSAL_RS15455 (Csal_3052) pilU 3401149..3402306 (-) 1158 WP_011508342.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CSAL_RS15460 (Csal_3053) pilT 3402348..3403388 (-) 1041 WP_011508343.1 type IV pilus twitching motility protein PilT Machinery gene
  CSAL_RS15465 (Csal_3054) - 3403424..3404131 (+) 708 WP_011508344.1 YggS family pyridoxal phosphate-dependent enzyme -
  CSAL_RS15470 (Csal_3055) proC 3404181..3405005 (+) 825 WP_011508345.1 pyrroline-5-carboxylate reductase -
  CSAL_RS15475 (Csal_3056) - 3405043..3405633 (+) 591 WP_011508346.1 YggT family protein -
  CSAL_RS15480 (Csal_3057) - 3405692..3406852 (+) 1161 WP_035414463.1 homoserine O-acetyltransferase -
  CSAL_RS15485 (Csal_3058) metW 3406852..3407463 (+) 612 WP_011508348.1 methionine biosynthesis protein MetW -
  CSAL_RS15490 (Csal_3059) sfsA 3407453..3408214 (-) 762 WP_011508349.1 DNA/RNA nuclease SfsA -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38117.60 Da        Isoelectric Point: 6.9913

>NTDB_id=25746 CSAL_RS15460 WP_011508343.1 3402348..3403388(-) (pilT) [Chromohalobacter israelensis DSM 3043]
MDIIELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPPIEDREVRKLIYAIMNDQQRRDYEEALEHDFSFDVPGVAR
FRVNVFHQGRGAGAVFRTIPSEVLTLDDLELGEVFRRLSSLPRGLVLVTGPTGSGKSTTLAAMIDYVNENYYQHVLTIED
PVEFVHRSKRSLVNQRELHRDTRSFATALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPGDEKSMVRSMLSESLQGVISQVLLKREGGGRVAAHEILVASGAVRNLIRENKLAQMYSSIQTGGSLGMQTLDAAL
SRLVSQGIVSRAEAQAKAKDGRVLGV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=25746 CSAL_RS15460 WP_011508343.1 3402348..3403388(-) (pilT) [Chromohalobacter israelensis DSM 3043]
ATGGATATTATCGAACTGCTGGCGTTCTCGGCAAAGCAGAAGGCCTCCGACCTGCACCTGTCCGCCGGGCTGCCGCCGAT
GATTCGCGTCGATGGCGACATCCGCCGGCTCAACGTGCCGCCGATCGAGGATCGTGAGGTCCGCAAGCTGATCTACGCGA
TCATGAACGATCAGCAGCGCCGCGACTACGAAGAGGCGCTCGAGCACGATTTCTCGTTCGATGTGCCGGGGGTGGCGCGC
TTCCGCGTCAACGTCTTTCACCAGGGGCGCGGTGCCGGCGCCGTCTTCCGCACCATTCCCAGCGAGGTGCTCACTCTCGA
CGACCTGGAACTGGGCGAGGTATTTCGCCGTCTGTCCTCCTTGCCGCGAGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGAGCACGACCTTGGCGGCGATGATCGACTACGTCAACGAAAACTACTACCAGCATGTGTTGACCATCGAGGAC
CCGGTGGAATTCGTGCACCGCAGCAAGCGCAGCCTCGTCAATCAGCGCGAGCTTCACCGCGACACGCGCAGCTTCGCCAC
GGCGTTGCGTTCGGCACTGCGCGAGGATCCGGACGTGATCCTGGTCGGCGAACTGCGCGACCTGGAGACCATCCGCCTGG
CGTTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGTACTCTGCACACCACGTCGGCCGCCAAGACGGTGGACCGCATC
ATCGATGTCTTTCCCGGCGACGAAAAAAGCATGGTGCGGTCGATGCTGTCCGAGTCGTTGCAAGGGGTGATCTCGCAGGT
GCTGCTCAAGCGTGAGGGTGGCGGGCGCGTGGCGGCGCACGAGATACTCGTCGCCAGCGGCGCGGTGCGAAACCTGATAC
GCGAAAACAAGCTGGCGCAGATGTACTCCTCGATCCAGACCGGCGGCAGCCTGGGCATGCAGACTCTGGATGCGGCGCTT
TCGCGGCTGGTGTCGCAGGGTATCGTTTCGCGTGCCGAGGCGCAGGCCAAGGCGAAGGACGGACGCGTGCTGGGTGTGTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1QT11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.106

97.977

0.746
  pilT Pseudomonas stutzeri DSM 10701

75.811

97.977

0.743
  pilT Acinetobacter baylyi ADP1

72.861

97.977

0.714
  pilT Acinetobacter nosocomialis M2

72.861

97.977

0.714
  pilT Acinetobacter baumannii strain A118

72.566

97.977

0.711
  pilT Acinetobacter baumannii D1279779

72.566

97.977

0.711
  pilT Legionella pneumophila strain Lp02

69.527

97.688

0.679
  pilT Legionella pneumophila strain ERS1305867

69.527

97.688

0.679
  pilT Neisseria meningitidis 8013

66.276

98.555

0.653
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.399

0.65
  pilT Vibrio cholerae strain A1552

66.766

97.399

0.65
  pilT Neisseria gonorrhoeae MS11

65.982

98.555

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.733

98.555

0.5
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.821

0.399
  pilU Vibrio cholerae strain A1552

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382

Multiple sequence alignment