Detailed information NTG0025745

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CSAL_RS15455
• Genome accession: NC_007963
• Coordinates: 3401149..3402306 (-)
• Length: 385 a.a.
• NCBI ID: WP_011508342.1
• Uniprot ID: -
• Organism: Chromohalobacter israelensis DSM 3043
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3396149..3407306

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CSAL_RS15445 (Csal_3050)	tyrS	3398731..3399927 (-)	1197	WP_011508340.1	tyrosine--tRNA ligase	-
CSAL_RS15450 (Csal_3051)	-	3400028..3401143 (+)	1116	WP_011508341.1	anhydro-N-acetylmuramic acid kinase	-
CSAL_RS15455 (Csal_3052)	pilU	3401149..3402306 (-)	1158	WP_011508342.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CSAL_RS15460 (Csal_3053)	pilT	3402348..3403388 (-)	1041	WP_011508343.1	type IV pilus twitching motility protein PilT	Machinery gene
CSAL_RS15465 (Csal_3054)	-	3403424..3404131 (+)	708	WP_011508344.1	YggS family pyridoxal phosphate-dependent enzyme	-
CSAL_RS15470 (Csal_3055)	proC	3404181..3405005 (+)	825	WP_011508345.1	pyrroline-5-carboxylate reductase	-
CSAL_RS15475 (Csal_3056)	-	3405043..3405633 (+)	591	WP_011508346.1	YggT family protein	-
CSAL_RS15480 (Csal_3057)	-	3405692..3406852 (+)	1161	WP_035414463.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42757.65 Da
• Isoelectric Point: 5.7759

>NTDB_id=25745 CSAL_RS15455 WP_011508342.1 3401149..3402306(-) (pilU) [Chromohalobacter israelensis DSM 3043]
MTPHEWLTQLLDIMLDKQASDLLVSTGAPPSLKIDGALVTLGDKAMSVGQVRELVSAALPQGVRERFEAEREANFALSVP
GKGRFRVSAFYQRSQMAMVVRRIAYDIPDLESLALPSALGELVNRQRGLVFIVGGTGSGKSTTLASMIQHRNQTTGGHVI
SIEDPIEYIHPHGRAIINQREVGIDTESFEVALKNALRQAPDVILIGEVRSREVMEHALTFAETGHLCLATLHANNANQA
LERIQHFFPSERHEQVWMDLSLNLQAIVAQRLLPREDGEGRCAAVEVMLRSPLISDLIRKGAIHDIKEVMQRSRDQGMQT
FDQALYALYRQGRISEKMALVHADSANDLRLQIAYGDAESPQLARAADAAERFTLKGDDEWREDE

Nucleotide

• Length: 1158 bp

>NTDB_id=25745 CSAL_RS15455 WP_011508342.1 3401149..3402306(-) (pilU) [Chromohalobacter israelensis DSM 3043]
ATGACGCCGCATGAATGGCTGACGCAACTGCTGGATATCATGCTCGACAAGCAGGCCTCCGATCTGCTCGTGTCCACGGG
GGCGCCGCCGAGTCTCAAGATCGATGGCGCACTGGTCACGCTGGGAGACAAGGCGATGTCGGTGGGGCAAGTGCGTGAAC
TGGTCTCGGCGGCGCTGCCTCAGGGGGTACGCGAACGCTTCGAGGCCGAGCGCGAGGCCAACTTTGCGCTCAGCGTGCCG
GGCAAGGGGCGCTTTCGTGTCAGCGCCTTCTACCAGCGTAGCCAGATGGCGATGGTCGTGCGGCGCATCGCCTACGATAT
CCCCGACCTGGAAAGCCTGGCCCTGCCCTCGGCGCTGGGGGAACTGGTCAATCGTCAGCGTGGTTTGGTATTCATCGTCG
GCGGCACGGGAAGCGGCAAGTCGACGACGCTGGCGTCGATGATCCAGCATCGCAACCAGACCACCGGGGGGCACGTGATC
AGCATCGAGGACCCCATCGAGTACATTCACCCTCACGGGCGGGCGATCATCAACCAGCGGGAAGTGGGCATCGATACCGA
GTCCTTCGAGGTGGCGCTCAAGAATGCGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGGTTCGTTCGCGCGAAG
TCATGGAGCATGCCCTGACCTTTGCCGAAACCGGGCATCTGTGCCTGGCGACGCTGCATGCCAATAACGCCAACCAGGCC
CTGGAGCGCATTCAGCACTTCTTTCCCAGCGAGCGCCACGAACAGGTGTGGATGGACCTTTCGCTCAACCTGCAGGCCAT
CGTCGCGCAGCGTCTGTTGCCTCGCGAGGATGGCGAGGGGCGGTGCGCGGCCGTCGAGGTGATGCTGCGCTCGCCGCTGA
TCAGCGACCTGATTCGCAAGGGAGCGATTCACGATATCAAGGAGGTCATGCAGCGCTCACGGGATCAGGGCATGCAGACC
TTCGACCAGGCCCTGTACGCGCTCTACCGACAAGGGCGGATCAGCGAAAAGATGGCGCTGGTGCATGCCGATTCCGCCAA
CGACCTGCGTCTGCAAATCGCCTATGGCGATGCCGAGAGCCCGCAACTGGCGAGGGCTGCCGATGCCGCCGAGCGCTTCA
CCCTGAAAGGCGATGACGAGTGGCGCGAGGATGAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (48-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.952	97.143	0.553
pilU	Acinetobacter baylyi ADP1	53.867	97.403	0.525
pilU	Vibrio cholerae strain A1552	57.349	90.13	0.517
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.023	89.091	0.392