Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SDEN_RS13940 Genome accession   NC_007954
Coordinates   3212747..3213784 (-) Length   345 a.a.
NCBI ID   WP_041405818.1    Uniprot ID   -
Organism   Shewanella denitrificans OS217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3207747..3218784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDEN_RS13910 (Sden_2675) yciH 3207786..3208115 (-) 330 WP_041405816.1 stress response translation initiation inhibitor YciH -
  SDEN_RS13915 (Sden_2676) - 3208452..3209009 (+) 558 WP_011497106.1 DUF4136 domain-containing protein -
  SDEN_RS13920 (Sden_2677) - 3209041..3209556 (-) 516 WP_011497107.1 GNAT family N-acetyltransferase -
  SDEN_RS13925 (Sden_2678) hemH 3209556..3210572 (-) 1017 WP_011497108.1 ferrochelatase -
  SDEN_RS13930 (Sden_2679) - 3210682..3211212 (-) 531 WP_232279971.1 glutathione peroxidase -
  SDEN_RS13935 (Sden_2680) pilU 3211623..3212735 (-) 1113 WP_011497110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SDEN_RS13940 (Sden_2681) pilT 3212747..3213784 (-) 1038 WP_041405818.1 type IV pilus twitching motility protein PilT Machinery gene
  SDEN_RS13945 (Sden_2682) - 3213819..3214526 (+) 708 WP_011497112.1 YggS family pyridoxal phosphate-dependent enzyme -
  SDEN_RS13950 (Sden_2683) proC 3214654..3215472 (+) 819 WP_011497113.1 pyrroline-5-carboxylate reductase -
  SDEN_RS13955 (Sden_2684) - 3215653..3216201 (+) 549 WP_041405819.1 YggT family protein -
  SDEN_RS13960 (Sden_2685) - 3216409..3216825 (+) 417 WP_232279972.1 DUF4426 domain-containing protein -
  SDEN_RS13965 (Sden_2686) rdgB 3217005..3217607 (+) 603 WP_011497116.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38269.87 Da        Isoelectric Point: 6.9128

>NTDB_id=25716 SDEN_RS13940 WP_041405818.1 3212747..3213784(-) (pilT) [Shewanella denitrificans OS217]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLEQSL
QVLVNRGLITREDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=25716 SDEN_RS13940 WP_041405818.1 3212747..3213784(-) (pilT) [Shewanella denitrificans OS217]
ATGGAAATTACTGAATTATTAGCCTTTAGCGTAAAACATAAGGCATCAGATCTTCACTTATCAGCAGGTGTATCGCCTAT
GATCCGAGTCGATGGCGAAGTCAGAAAAATAAATCTGCCGGCGCTGGATCATCAAGGCGTTCACAGCCTAGTGTACGATA
TAATGAATGATAAACAACGCAAAGACTATGAAGAGCACCTAGAAATCGATTTTTCCTTCGAAGTGCCTAATTTAGCTCGC
TTTCGTGTTAATGCCTTTAATCAGTCTCGTGGCGCGGGCGCAGTATTTCGTACGATCCCCAGTGATATTTTATCCCTTGA
GCAGTTAGGTGCTCCTGAAATTTTTAAGAAAATCGCAAGCTTCCCAAGGGGGCTGGTGTTGGTGACTGGGCCCACTGGCT
CGGGTAAATCAACCACCTTGGCAGCCATGGTGGATTATGTGAATGAAAATCGTCACGATCACATCTTAACCATCGAAGAC
CCCATAGAATTTGTGCATCAAAACAAGCAATGTTTGATTAACCAGCGTGAAGTGCATAAACACACCCACAGCTTTAATGC
GGCGTTACGTTCGGCGCTGCGTGAAGATCCTGATGTTATTCTCGTGGGTGAAATGCGAGATCTTGAGACCATACGCCTTG
CCATGACGGCGGCGGAAACTGGTCATTTAGTGTTTGGCACCTTGCATACTACGTCAGCTGCCAAGACCATTGACCGTGTG
GTCGACGTATTCCCCGCGGGTGAAAAAGACATGGTGCGCACCATGTTGTCAGAATCCTTACAGGCGGTTATCTCGCAGAC
CTTGATCAAGAAAATTGGCGGCGGCCGGGTTGCCGCCCATGAAATCATGATGGGCACCCCGGCTATTCGTAACCTCATTC
GTGAAGATAAAGTGGCACAAATGTACTCAGCGATTCAAACGGGTATGTCTCACGGGATGCAAACCTTGGAGCAGAGCCTG
CAAGTGTTAGTGAATCGTGGTCTTATTACTCGTGAAGATGCCATGTCTAAGAGCTCAAATAAACAAGCTAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.883

99.42

0.794
  pilT Acinetobacter baumannii D1279779

79.883

99.42

0.794
  pilT Acinetobacter baumannii strain A118

79.883

99.42

0.794
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

80.547

95.362

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.667

97.391

0.406
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394

Multiple sequence alignment