Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SDEN_RS13935 Genome accession   NC_007954
Coordinates   3211623..3212735 (-) Length   370 a.a.
NCBI ID   WP_011497110.1    Uniprot ID   Q12KR7
Organism   Shewanella denitrificans OS217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3206623..3217735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SDEN_RS13900 (Sden_2673) ruvX 3206624..3207049 (-) 426 WP_011497103.1 Holliday junction resolvase RuvX -
  SDEN_RS13905 (Sden_2674) - 3207207..3207761 (-) 555 WP_011497104.1 YqgE/AlgH family protein -
  SDEN_RS13910 (Sden_2675) yciH 3207786..3208115 (-) 330 WP_041405816.1 stress response translation initiation inhibitor YciH -
  SDEN_RS13915 (Sden_2676) - 3208452..3209009 (+) 558 WP_011497106.1 DUF4136 domain-containing protein -
  SDEN_RS13920 (Sden_2677) - 3209041..3209556 (-) 516 WP_011497107.1 GNAT family N-acetyltransferase -
  SDEN_RS13925 (Sden_2678) hemH 3209556..3210572 (-) 1017 WP_011497108.1 ferrochelatase -
  SDEN_RS13930 (Sden_2679) - 3210682..3211212 (-) 531 WP_232279971.1 glutathione peroxidase -
  SDEN_RS13935 (Sden_2680) pilU 3211623..3212735 (-) 1113 WP_011497110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SDEN_RS13940 (Sden_2681) pilT 3212747..3213784 (-) 1038 WP_041405818.1 type IV pilus twitching motility protein PilT Machinery gene
  SDEN_RS13945 (Sden_2682) - 3213819..3214526 (+) 708 WP_011497112.1 YggS family pyridoxal phosphate-dependent enzyme -
  SDEN_RS13950 (Sden_2683) proC 3214654..3215472 (+) 819 WP_011497113.1 pyrroline-5-carboxylate reductase -
  SDEN_RS13955 (Sden_2684) - 3215653..3216201 (+) 549 WP_041405819.1 YggT family protein -
  SDEN_RS13960 (Sden_2685) - 3216409..3216825 (+) 417 WP_232279972.1 DUF4426 domain-containing protein -
  SDEN_RS13965 (Sden_2686) rdgB 3217005..3217607 (+) 603 WP_011497116.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40970.96 Da        Isoelectric Point: 6.0526

>NTDB_id=25715 SDEN_RS13935 WP_011497110.1 3211623..3212735(-) (pilU) [Shewanella denitrificans OS217]
MDVIPFLKVMVERKASDLFITAGFPPSAKIDGELRQLTEESLNPAQSLAFVEALMTDVQKKEFHDTSECNFAFAEKELGR
FRVSAFWQRESAGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHSRGHILTIED
PVEFVHDHRRSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVAKSDGSGRRAAIEVLINTPRVSSIIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYAEGEISYADALHHADSPNDLRLMIKLQNNETGGSGLMDGVTLNMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=25715 SDEN_RS13935 WP_011497110.1 3211623..3212735(-) (pilU) [Shewanella denitrificans OS217]
ATGGATGTTATTCCTTTTTTAAAAGTCATGGTGGAGCGCAAAGCCTCGGATCTCTTCATTACCGCAGGTTTTCCACCAAG
CGCGAAAATCGATGGTGAACTACGCCAATTAACCGAAGAAAGCTTGAATCCGGCGCAGTCACTGGCCTTTGTCGAAGCCT
TGATGACGGATGTACAGAAAAAAGAGTTTCACGACACCAGTGAGTGTAACTTTGCCTTTGCAGAAAAAGAATTAGGTCGA
TTTCGTGTTAGCGCCTTTTGGCAGCGAGAATCTGCAGGCTGCGTGATGCGCCGCATCGAGACAAAAATCCCTGAGGTGGA
TGACTTAAAATTACCGCCAATTTTAAAAGATTTAGTCATGAGTAAACGCGGCCTTATCATCATGGTGGGCGGTACGGGTA
CGGGTAAGTCGACATCACTTGCAGCGTTAGTGGGCTATCGTAACGCCCATTCAAGGGGGCATATATTAACCATCGAAGAT
CCGGTGGAATTTGTCCACGATCATAGACGCAGCATCATCACCCAGCGAGAGGTGGGCATAGATACAGAATCTTTTGATGC
GGCGCTTAAAAGCTCGCTCCGTCAAGCCCCTGATGTGATTTTAATTGGTGAAATTCGCAGCCAAGAAACCATGGAGTTTG
CACTGTCGTTCGCTGAAACTGGTCACCTGTGTATGGCAACCTTGCACGCAAACAATGCCAACCAGGCGCTCGATCGCATC
ATGCACTTGGTACCAGAAAGTAAGCATCATCAATTGCTGTTCGATTTGTCACTGAACCTAAGGGGCATAGTAGCGCAGCA
GTTAGTGGCAAAATCTGACGGTAGCGGACGTCGCGCCGCGATCGAAGTGCTGATCAACACCCCTAGGGTGTCCAGCATTA
TCGCGAAGAATGAGCTGCACTTACTTAAAGAAACCATGGCAAAGTCAAACGAGCAAGGCATGCAGACCTTCGATCAGGCC
TTACTTAAACTCTACGCCGAGGGTGAAATTAGTTATGCCGATGCTTTACACCATGCAGATTCTCCTAACGATTTACGCTT
GATGATTAAACTGCAAAATAATGAAACCGGTGGCAGTGGCTTAATGGATGGTGTGACCTTAAATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q12KR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.409

100

0.597
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.167

97.297

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

89.189

0.37
  pilT Vibrio cholerae strain A1552

41.515

89.189

0.37
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment