Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BPRO_RS01970 Genome accession   NC_007948
Coordinates   408468..409511 (-) Length   347 a.a.
NCBI ID   WP_011481367.1    Uniprot ID   Q12GI2
Organism   Polaromonas sp. JS666     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 403468..414511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPRO_RS01945 (Bpro_0391) - 404221..404670 (+) 450 WP_011481362.1 YraN family protein -
  BPRO_RS01950 (Bpro_0392) - 404810..405409 (+) 600 WP_011481363.1 phosphoheptose isomerase -
  BPRO_RS01955 (Bpro_0393) - 405406..406074 (+) 669 WP_011481364.1 BON domain-containing protein -
  BPRO_RS01960 (Bpro_0394) - 406162..407076 (-) 915 WP_011481365.1 NAD(P)-dependent oxidoreductase -
  BPRO_RS01965 (Bpro_0395) pilU 407163..408299 (-) 1137 WP_011481366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BPRO_RS01970 (Bpro_0396) pilT 408468..409511 (-) 1044 WP_011481367.1 type IV pilus twitching motility protein PilT Machinery gene
  BPRO_RS01975 (Bpro_0397) - 409562..410278 (+) 717 WP_011481368.1 YggS family pyridoxal phosphate-dependent enzyme -
  BPRO_RS01980 (Bpro_0398) - 410442..410993 (-) 552 WP_011481369.1 DUF3617 domain-containing protein -
  BPRO_RS01985 (Bpro_0399) - 411090..411569 (-) 480 WP_011481370.1 Lrp/AsnC ligand binding domain-containing protein -
  BPRO_RS01990 (Bpro_0400) - 411641..412066 (-) 426 WP_011481371.1 Lrp/AsnC family transcriptional regulator -
  BPRO_RS01995 (Bpro_0401) - 412468..413232 (+) 765 WP_011481372.1 ABC transporter substrate-binding protein -
  BPRO_RS02000 (Bpro_0402) - 413270..414058 (+) 789 WP_041388234.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38138.89 Da        Isoelectric Point: 6.8355

>NTDB_id=25673 BPRO_RS01970 WP_011481367.1 408468..409511(-) (pilT) [Polaromonas sp. JS666]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQRKNYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLALKPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTELVKRNVISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=25673 BPRO_RS01970 WP_011481367.1 408468..409511(-) (pilT) [Polaromonas sp. JS666]
ATGGACATCACGCAGCTGCTGGCCTTTAGCGTCAAAAACAAGGCGTCCGACTTGCATCTGTCGGCAGGTTTGCCGCCCAT
GATCCGCGTTCATGGCGACGTGCGGCGCATCAACATCGACCCGCTGGACCACAAGCAGGTGCATGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGAACTATGAAGAGTTCCTGGAGGTGGACTTCTCCTTTGAGATTGACGGACTGGCACGC
TTTCGCGTCAATGCCTTCAACCAGAACCGGGGTGCCGGTGCCGTGCTACGAACCATTCCCTCCAAAATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCCTTGGCGACCTGGCGCTCAAGCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTTCCTGAATGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTTGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGCCCGCACACGCTGAGCTTCAACGC
CGCTCTCAAGTCCGCCCTGCGCGAAGACCCGGACGCGATTCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CCATGTCGGCCGCAGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACGTCAAGCGCCGCCAAGACGATTGACCGCATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGATCCGCTCCATGCTGTCGGAGTCGCTGCAGGCCGTCATTTCGCAAAC
GCTGTGCAAGACCAAGGACGGCGCGGGCCGCGTGGCCGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGTGAGGCGAAGGTCGCGCAGATGTACTCGTCCATCCAGACCGGCAACAGCGTGGGCATGCAAACGCTGGACCAGAAC
CTGACCGAGCTGGTCAAGCGCAACGTGATTTCGCCCGCGGAAGCGCGCGGCAAGGCGAAAATACCGGAAAACTTTCCAGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q12GI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.982

98.271

0.648
  pilT Vibrio cholerae strain A1552

65.982

98.271

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment