Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BPRO_RS01965 Genome accession   NC_007948
Coordinates   407163..408299 (-) Length   378 a.a.
NCBI ID   WP_011481366.1    Uniprot ID   -
Organism   Polaromonas sp. JS666     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 402163..413299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPRO_RS01940 (Bpro_0390) rsmI 403331..404170 (-) 840 WP_011481361.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  BPRO_RS01945 (Bpro_0391) - 404221..404670 (+) 450 WP_011481362.1 YraN family protein -
  BPRO_RS01950 (Bpro_0392) - 404810..405409 (+) 600 WP_011481363.1 phosphoheptose isomerase -
  BPRO_RS01955 (Bpro_0393) - 405406..406074 (+) 669 WP_011481364.1 BON domain-containing protein -
  BPRO_RS01960 (Bpro_0394) - 406162..407076 (-) 915 WP_011481365.1 NAD(P)-dependent oxidoreductase -
  BPRO_RS01965 (Bpro_0395) pilU 407163..408299 (-) 1137 WP_011481366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BPRO_RS01970 (Bpro_0396) pilT 408468..409511 (-) 1044 WP_011481367.1 type IV pilus twitching motility protein PilT Machinery gene
  BPRO_RS01975 (Bpro_0397) - 409562..410278 (+) 717 WP_011481368.1 YggS family pyridoxal phosphate-dependent enzyme -
  BPRO_RS01980 (Bpro_0398) - 410442..410993 (-) 552 WP_011481369.1 DUF3617 domain-containing protein -
  BPRO_RS01985 (Bpro_0399) - 411090..411569 (-) 480 WP_011481370.1 Lrp/AsnC ligand binding domain-containing protein -
  BPRO_RS01990 (Bpro_0400) - 411641..412066 (-) 426 WP_011481371.1 Lrp/AsnC family transcriptional regulator -
  BPRO_RS01995 (Bpro_0401) - 412468..413232 (+) 765 WP_011481372.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41804.16 Da        Isoelectric Point: 6.9720

>NTDB_id=25672 BPRO_RS01965 WP_011481366.1 407163..408299(-) (pilU) [Polaromonas sp. JS666]
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARAIMNDKQAAEFERTKECNFAI
SPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQVLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNSQRAKSTDLAAGTESFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=25672 BPRO_RS01965 WP_011481366.1 407163..408299(-) (pilU) [Polaromonas sp. JS666]
ATGGAGCGCGATCAAGCCAGTAAATTCATCCACGACTTGCTGAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACCGGCGACTTTCCGCCGGCCATGAAGGTGGACGGCAAAATAACCAAAGTCTCGCCACAGCCGCTCAATGCCGGCCACA
CGCTGGCCCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCGGAGTTTGAACGCACCAAGGAATGCAATTTCGCCATT
TCGCCGCCAGGCGTGGGGCGCTTTCGCGTAAACGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTGATGCGGACGATCCC
GGCCGTGCTGCCCACGATTGACGGGCTGGGCGTGCCCCAGGTGCTCAAGGATGTGGTGATGAGCAAGCGGGGGCTGACGA
TTCTCGTCGGCGCCACCGGTTCGGGCAAGTCCACCACACTGGCGGCCATGGTGGACTGGCGCAACGAACAGTCATTCGGC
CACATCATCACGATTGAAGACCCGGTGGAGTTTGTCCATGCGCACAAGAACTGCGTGATCACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCGGCGCTGAAAAACACCCTGCGCCAGGCGCCTGATGTGATCCTGATGGGCGAGATCCGTG
ACCGTGAAACCATGGAGCATGCCATCGCCTTTGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCGTGATCAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGATGGATTTGTCGCTCAACCT
CAAGGCCATGATTTCGCAGCGCCTGGTCCCCAAGCAGGATGGCAAGGGCCGCTTTGCGGCGGTCGAGGTCATGCTCAATT
CACCGCTGATCTCCGACCTGATCTTCAAGGGCGATGTTTCCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTCGGC
ATGCAGACTTTCGATCAGGCGCTGTACGACGCCTTTGAAAGCCACTTCATCACCTACGAGGATGCACTGCGCAACGCCGA
TTCGGTGAACGACCTGCGTCTGCAGATCAAGCTCAATTCGCAGCGCGCCAAGTCGACCGACCTTGCCGCGGGCACCGAGA
GTTTCGCCATCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.037

92.328

0.582
  pilU Acinetobacter baylyi ADP1

57.423

94.444

0.542
  pilU Vibrio cholerae strain A1552

54.545

90.212

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter nosocomialis M2

44.828

84.392

0.378
  pilT Acinetobacter baumannii D1279779

44.828

84.392

0.378
  pilT Acinetobacter baumannii strain A118

44.828

84.392

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.862

0.368
  pilT Vibrio cholerae strain A1552

43.849

83.862

0.368
  pilT Acinetobacter baylyi ADP1

42.947

84.392

0.362
  pilT Legionella pneumophila strain Lp02

41.141

88.095

0.362
  pilT Legionella pneumophila strain ERS1305867

41.141

88.095

0.362

Multiple sequence alignment