Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MFLA_RS10785 Genome accession   NC_007947
Coordinates   2255067..2256110 (-) Length   347 a.a.
NCBI ID   WP_011480328.1    Uniprot ID   Q1GZG3
Organism   Methylobacillus flagellatus KT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2250067..2261110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS10765 (Mfla_2103) - 2250140..2251414 (+) 1275 WP_011480324.1 dihydroorotase -
  MFLA_RS10770 (Mfla_2104) - 2251467..2252918 (-) 1452 WP_011480325.1 acetamidase/formamidase family protein -
  MFLA_RS10775 (Mfla_2105) - 2253107..2253859 (-) 753 WP_011480326.1 TerC family protein -
  MFLA_RS10780 (Mfla_2106) pilU 2253911..2255047 (-) 1137 WP_011480327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MFLA_RS10785 (Mfla_2107) pilT 2255067..2256110 (-) 1044 WP_011480328.1 type IV pilus twitching motility protein PilT Machinery gene
  MFLA_RS10790 (Mfla_2108) - 2256187..2256894 (+) 708 WP_048811698.1 YggS family pyridoxal phosphate-dependent enzyme -
  MFLA_RS10795 (Mfla_2109) proC 2256891..2257700 (+) 810 WP_011480330.1 pyrroline-5-carboxylate reductase -
  MFLA_RS10800 (Mfla_2110) - 2257737..2258312 (+) 576 WP_011480331.1 YggT family protein -
  MFLA_RS10805 (Mfla_2111) - 2258384..2259106 (-) 723 WP_011480332.1 NADPH-dependent F420 reductase -
  MFLA_RS10810 (Mfla_2112) rng 2259254..2260705 (-) 1452 WP_011480333.1 ribonuclease G -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38239.90 Da        Isoelectric Point: 6.6599

>NTDB_id=25661 MFLA_RS10785 WP_011480328.1 2255067..2256110(-) (pilT) [Methylobacillus flagellatus KT]
MDISDLLAFSVKNKASDLHLSAGMPPLIRVHGDVRKINVPPLDHSAVHDMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEQLGCPPIFREIAQNPRGIVLVTGPTGSGKSTTLAAMIDFINENEFGHILTVED
PIEFVHTSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQN
LQDLVRRNVISLAEARAKAANKDAFVG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=25661 MFLA_RS10785 WP_011480328.1 2255067..2256110(-) (pilT) [Methylobacillus flagellatus KT]
TTGGATATTTCTGATTTGCTGGCGTTTTCGGTCAAAAACAAGGCTTCCGATTTGCACCTCTCGGCGGGCATGCCGCCATT
GATCCGGGTGCATGGCGATGTGCGCAAGATCAACGTGCCGCCGCTGGACCATAGCGCCGTGCACGACATGGTGTACGACA
TCATGAATGACGGTCAGCGCAAGGTATACGAAGAAACGCTGGAGTGCGATTTTTCCTTCGAGATTCCCAACCTGGCGCGT
TTCCGCGTCAATGCCTTCAATCAGAACCGCGGTGCGGGTGCGGTGTTCCGTACCATTCCCTCCAAGGTGCTGACCATGGA
GCAGCTCGGCTGTCCTCCCATCTTTCGCGAGATTGCGCAAAATCCGCGCGGCATCGTGTTGGTGACCGGGCCAACCGGCT
CCGGTAAATCCACTACGCTGGCGGCGATGATCGACTTCATCAATGAAAACGAGTTTGGTCATATCCTCACCGTGGAAGAC
CCGATCGAGTTCGTGCATACCTCGAAGAAATGCCTGATCAACCAGCGTGAGGTCGGGCCGCATACCTTGTCATTCTCAAA
CGCCCTGCGCTCTGCCCTGCGCGAGGACCCGGATGTGATCCTGGTCGGCGAAATGCGCGATCTGGAGACCATCCGCCTGG
CACTCAGCGCCGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCAGCCAAGACCATAGACCGTATC
GTCGACGTGTTCCCCGCGGCGGAAAAGGAAATGGTGCGGTCCATGTTGTCCGAATCACTGCGTGCCGTGATCTCACAGAC
CTTGTGCAAGACCAAGGATGAGCAAGGCCGCGTGGCGGCGCACGAAATCATGATCGGTACGCCCGCGATCCGCAACTTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCCGCGATCCAGACCGGGCAGAACGTCGGCATGCAGACGCTAGACCAGAAC
CTGCAGGACCTCGTGCGACGCAATGTCATTTCCCTGGCCGAGGCACGGGCCAAGGCCGCCAATAAAGATGCCTTCGTCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1GZG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.457

99.712

0.772
  pilT Acinetobacter baumannii D1279779

77.457

99.712

0.772
  pilT Acinetobacter baumannii strain A118

77.457

99.712

0.772
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

70.725

99.424

0.703
  pilT Neisseria gonorrhoeae MS11

70.435

99.424

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Acinetobacter baylyi ADP1

43.235

97.983

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment