Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MFLA_RS10780 Genome accession   NC_007947
Coordinates   2253911..2255047 (-) Length   378 a.a.
NCBI ID   WP_011480327.1    Uniprot ID   -
Organism   Methylobacillus flagellatus KT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2248911..2260047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS10760 (Mfla_2102) - 2249190..2250143 (+) 954 WP_011480323.1 aspartate carbamoyltransferase catalytic subunit -
  MFLA_RS10765 (Mfla_2103) - 2250140..2251414 (+) 1275 WP_011480324.1 dihydroorotase -
  MFLA_RS10770 (Mfla_2104) - 2251467..2252918 (-) 1452 WP_011480325.1 acetamidase/formamidase family protein -
  MFLA_RS10775 (Mfla_2105) - 2253107..2253859 (-) 753 WP_011480326.1 TerC family protein -
  MFLA_RS10780 (Mfla_2106) pilU 2253911..2255047 (-) 1137 WP_011480327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MFLA_RS10785 (Mfla_2107) pilT 2255067..2256110 (-) 1044 WP_011480328.1 type IV pilus twitching motility protein PilT Machinery gene
  MFLA_RS10790 (Mfla_2108) - 2256187..2256894 (+) 708 WP_048811698.1 YggS family pyridoxal phosphate-dependent enzyme -
  MFLA_RS10795 (Mfla_2109) proC 2256891..2257700 (+) 810 WP_011480330.1 pyrroline-5-carboxylate reductase -
  MFLA_RS10800 (Mfla_2110) - 2257737..2258312 (+) 576 WP_011480331.1 YggT family protein -
  MFLA_RS10805 (Mfla_2111) - 2258384..2259106 (-) 723 WP_011480332.1 NADPH-dependent F420 reductase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42107.39 Da        Isoelectric Point: 6.5067

>NTDB_id=25660 MFLA_RS10780 WP_011480327.1 2253911..2255047(-) (pilU) [Methylobacillus flagellatus KT]
MDQGQAEKFVFDLLRLMLGKKASDLFITAAYPPAMKIDGRMTPVTQQALTPQQVREIARAIMNDKQAAEFEATRECNFAI
GLPGVARFRVNAFVQRGSVGMVFRTITTKIPRLEELGLPEVLKDVAQSKRGLVIFVGGTGSGKSTSLAAMVGYRNENSHG
HIITIEDPIEFVHEHKNCIITQREVGVDTDNWHIALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRHQLLMDLSLNVKAFVSQRLIARQDGKGRVAATEIMINSPLISDLIFKGDVHEIKEIIAKSRELG
MQTFDQSLFDLHEAGLITYEDALRNADSVNDLRLRIKLEGKDAKDRDLSAGLDSLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=25660 MFLA_RS10780 WP_011480327.1 2253911..2255047(-) (pilU) [Methylobacillus flagellatus KT]
ATGGATCAAGGGCAAGCGGAGAAATTTGTATTTGATCTGTTGCGGTTGATGCTGGGTAAGAAGGCATCCGACCTCTTCAT
TACGGCCGCCTATCCGCCCGCGATGAAGATTGACGGCAGGATGACACCAGTGACGCAGCAGGCATTGACGCCGCAACAGG
TGCGCGAGATCGCTCGCGCCATCATGAATGACAAGCAGGCAGCAGAGTTCGAAGCGACGCGTGAGTGCAATTTTGCCATC
GGCTTGCCCGGCGTGGCGCGTTTCCGCGTTAATGCCTTTGTGCAGCGTGGCAGCGTGGGCATGGTGTTCCGCACCATTAC
CACCAAGATTCCTCGCCTCGAAGAGCTTGGGCTGCCTGAAGTGCTCAAGGATGTGGCACAGTCCAAGCGGGGGCTGGTGA
TCTTCGTCGGCGGCACCGGTTCAGGGAAATCGACGTCGCTCGCGGCCATGGTCGGTTACCGCAACGAAAACAGCCATGGC
CACATCATCACCATCGAAGACCCGATCGAGTTTGTGCATGAGCACAAGAACTGCATCATCACTCAGCGCGAGGTCGGTGT
CGACACCGATAATTGGCATATCGCGCTCAAGAATACCTTGCGCCAGGCGCCCGACGTGATCCTCATCGGCGAGATCCGCG
ACCGAGAAACCATGGATTACGCGATTGCCTTCGCCGAGACCGGCCACCTGTGTATGGCAACGCTGCATGCCAACAGCACT
AACCAGGCATTGGACCGCATCATCAACTTTTTCCCGGAAGAGCGGCGGCACCAACTGCTGATGGATTTGTCGCTCAATGT
GAAGGCTTTTGTCTCCCAACGCCTGATAGCGCGTCAAGATGGCAAGGGGAGGGTGGCTGCCACGGAAATCATGATCAATT
CCCCGTTGATTTCCGACCTGATCTTCAAGGGAGACGTGCACGAAATCAAGGAAATCATTGCCAAATCGCGAGAGCTGGGC
ATGCAGACGTTTGACCAGTCGTTGTTTGACTTGCACGAGGCAGGACTCATCACCTACGAGGATGCACTGCGTAATGCCGA
CTCGGTCAATGACCTCAGGCTCAGGATCAAGCTGGAAGGCAAGGATGCAAAAGACCGGGATCTTTCTGCAGGGCTGGATA
GTCTGGGTATAGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.451

91.534

0.59
  pilU Acinetobacter baylyi ADP1

60.224

94.444

0.569
  pilU Vibrio cholerae strain A1552

56.647

91.534

0.519
  pilT Pseudomonas aeruginosa PAK

46.291

89.153

0.413
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

44.807

89.153

0.399
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baylyi ADP1

44.745

88.095

0.394
  pilT Vibrio cholerae strain A1552

44.277

87.831

0.389
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.277

87.831

0.389
  pilT Neisseria meningitidis 8013

42.353

89.947

0.381
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Neisseria gonorrhoeae MS11

41.888

89.683

0.376

Multiple sequence alignment